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  • 1
    ISSN: 1573-2568
    Keywords: acute viral hepatitis A ; Q fever ; fibrin ring hepatic granulomas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fibrin ring granuloma is characterized by a fibrinous ring surrounding a central fat vacuole. It has been found in the liver and bone marrow of patients with Q fever, and occasionally with visceral leishmaniasis, cytomegalovirus, Epstein-Barr virus,Staphylococcus epidermidis infections, Hodgkin's lymphoma, and hypersensitivity to allopurinol. We describe a case of serologically confirmed viral hepatitis A with this lesion in the liver biopsy. A false positive anti-hepatitis A virus IgM result has been excluded. This is, to our knowledge, the second reported case of type A hepatitis with hepatic fibrin ring granulomas. It confirms that hepatitis A should be included in the differential diagnosis of this lesion.
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  • 2
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have reviewed our experience with 22 patients with nonspecific inflammatory involvement of the small and large bowel. Patients with this combination of lesions usually have long-standing and intractable illnesses, with many complications, frequently resulting in multiple operations. In long-term follow-up we found a mortality of 23%. A particularly troublesome combination is granulomatous disease of the small bowel accompanying or followed by ulcerative colitis. Some suggested guidelines as to therapy in the management of these difficult cases have been presented. The term ileocolitis should be qualified, whenever possible, by the more specific pathologic terms “granulomatous” or “ulcerative” as an aid in planning therapy and in making a prognosis. In the occasional case with both of these characteristics, we subscribe to the term “mixed enterocolitis.”
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  • 3
    ISSN: 1573-269X
    Keywords: Magnetic levitation ; fuzzy logic control ; nonlinear systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This paper deals with the control in both horizontal and vertical directions of a nonlinear magnetic spherical particle suspension. A pair of horizontal electromagnets were added to the typical magnetic levitation system in order to handle the horizontal unexpected displacements of the particle. A fuzzy logic controller was designed to bring the particle to the operating location situated on the symmetry axis. Simulations with different initial position conditions of the particle are provided. It was shown that the controller has a good behavior for almost any initial conditions.
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  • 4
    ISSN: 0268-2605
    Keywords: polymer ; organotin ; hydride ; reduction ; pollution ; ICP-MS ; GC/FPD ; supported reagent ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: New polymer-supported organotin reagents have been prepared. The reducing ability of a polystyrene-supported organotin hydride was evaluated by reaction with haloalkanes. The level of organotin pollution was monitored in comparison with that generated by Bu3SnH, using ICP-MS analysis.
    Additional Material: 4 Ill.
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  • 5
    ISSN: 1432-1351
    Keywords: Key words Synaptic efficacy ; Paired pulse facilitation ; Neuromuscular junction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Under physiological conditions, the laryngeal synapse of male Xenopus laevis exhibits marked facilitation during repetitive nerve stimulation. The male laryngeal synapse is weak and requires facilitation to produce muscle action potentials and ultimately sound. The female laryngeal synapse is strong: muscle contractions are produced to single nerve stimuli. We sought to determine if laryngeal synapses of males and females also differ in their ability to facilitate. To measure facilitation, laryngeal muscle action potentials were suppressed either postsynaptically by bathing the preparation in saline containing curare or presynaptically by bathing the preparation in reduced calcium/elevated magnesium saline. Facilitation of postsynaptic potential amplitude or quantal content in response to paired pulses was measured in male and female larynges: there is no sex difference in paired pulse facilitation. Facilitation in response to trains of stimuli, in curare-blocked preparations, increased and reached plateau values more rapidly in females than in males, although the facilitation between the last and first pulses in the train was the same in the sexes. Thus, the sexually differentiated behavior of this synapse is controlled more by a sex difference in synaptic strength than by a sex difference in the ability to facilitate.
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  • 6
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Enzyme-gold complexes have been prepared with an endo β 1→4 xylanase (EC 3.2.1.8) and a β 1→4 mannanase (EC 3.2.1.78). The complexes were applied to ultrathin sections of plant cell walls for the ultrastructural localization of xylans in different tissues of a graminea and for the localization of glucomannans in the tracheids of spruce wood. The method proved to be highly specific and gave a very good contrast of the substrate polysaccharides. Used in conjunction with other cytochemical staining the enzyme-gold labelling provided information about the relative distribution of pectic polymers and xylans in primary walls.
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  • 7
    ISSN: 1432-1238
    Keywords: Serum phosphate ; Hypophosphatemia ; Critical ill patients ; Surgery ; Epidemiology ; Risk factors ; Mortality ; Hemodynamics ; Myocardial performance ; Treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objective To determine the incidence of hypophosphatemia in a surgical intensive care unit and to determine whether or not a phosphorus challenge causes a change in cardiac performance in hypophosphatemic patients. Design Prospective clinical study and case reports. Setting Surgical intensive care unit in an universitu hospital. Patients A total of 208 consecutive patients admitted to the surgical ICU were evaluated over a 6 months period. Interventions All classical risk factors for hypophosphatemia were recorded. A group of 8 moderate or severe hypophosphatemic patients were evaluated for hemodynamic data before and after a phosphorus load. Glucose phosphate was given over 30 min by the intravenous route. Dosage regimen was 0.4 mmol/kg weight for moderate hypophosphatemia and 0.8 mmol/kg weight for severe hypophosphatemia. Results Risk factors were present in 134 patients and 60 patients were hypophosphatemic (44.8%). Only 3 risk factors were discriminant for hypophosphatemia: sepsis, diuretics and total parenteral nutrition. The mortality was higher in the hypophosphatemic group than in the normophosphatemic group (30% versus 15.2%;p〈0.05). Cardiac performance improved after phosphatemia normalization in all patients (cardiac index: 3.82±1.87 versus 4.52±1.83 l/min·m2;p〈0.01). Conclusion This study underlines the high incidence (28.8%) of hypophosphatemia in surgical intensive care patients and its association with a high mortality rate (30%). A short course of phosphotherapy improves cardiac index (+18%).
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  • 8
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary α-Linked d-xylosyl side chains represent the typical feature common to all xyloglucans not shared by other cell wall polysaccharides. Since no easily available α-d-xyloxidase is known, advantage was taken of the conformational and configurational homologies between α-d-xylopyranose and α-d-glucopyranose to make an α-d-glucosidase-gold complex which was able to recognize α-d-xylosyl terminal residues of xyloglucans. This marker was used together with α-l-fucosidase gold complex for the double labeling on two different structural features of the same macromolecule in plant primary cell wall.
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  • 9
    ISSN: 1432-2048
    Keywords: Key words: Casparian strip ; Endodermis ; Hypodermis ; Lignin ; Root ; Suberin ; Zea (cell walls)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The composition of suberin and lignin in endodermal cell walls (ECWs) and in rhizodermal/hypodermal cell walls (RHCWs) of developing primary maize (Zea mays L.) roots was analysed after depolymerisation of enzymatically isolated cell wall material. Absolute suberin amounts related to root length significantly increased from primary ECWs (Casparian strips) to secondary ECWs (suberin lamella). During further maturation of the endodermis, reaching the final tertiary developmental state characterised by the deposition of lignified secondary cell walls (u-shaped cell wall deposits), suberin amounts remained constant. Absolute amounts of lignin related to root length constantly increased throughout the change from primary to tertiary ECWs. The suberin of Casparian strips contained high amounts of carboxylic and 2-hydroxy acids, and differed substantially from the suberin of secondary and tertiary ECWs, which was dominated by high contents of ω-hydroxycarboxylic and 1,ω-dicarboxylic acids. Furthermore, the chain-length distribution of suberin monomers in primary ECWs ranged from C16 to C24, whereas in secondary and tertiary ECWs a shift towards higher chain lengths (C16 to C28) was observed. The lignin composition of Casparian strips (primary ECWs) showed a high syringyl content and was similar to lignin in secondary cell walls of the tertiary ECWs, whereas lignin in secondary ECWs contained higher amounts of p-hydroxyphenyl units. The suberin and lignin compositions of RHCWs rarely changed with increasing root age. However, compared to the suberin in ECWs, where C16 and C18 were the most prominent chain lengths, the suberin of RHCWs was dominated by the higher chain lengths (C24 and C26). The composition of RHCW lignin was similar to that of secondary-ECW lignin. Using lignin-specific antibodies, lignin epitopes were indeed found to be located in the Casparian strip. Surprisingly, the mature suberin layers of tertiary ECWs contained comparable amounts of lignin-like epitopes.
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  • 10
    ISSN: 1432-2048
    Keywords: Coleoptile (lignin formation) ; Hydrogen peroxide (lignin formation) ; Hydroxyproline-rich glycoprotein (maize coleoptile) ; Lignin (maize coleoptile) ; Phytochrome (lignin formation) ; Zea (lignin formation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lignin is an integral constituent of the primary cell walls of the dark-grown maize (Zea mays L.) coleoptile, a juvenile organ that is still in the developmental state of rapid cell extension. Coleoptile lignin was characterized by (i) conversion to lignothiolglycolate derivative, (ii) isolation of polymeric fragments after alkaline hydrolysis, (iii) reactivity to antibodies against dehydrogenative polymers prepared from monolignols, and (iv) identification of thioacidolysis products typical of lignins. Substantial amounts of lignin could be solubilized from the coleoptile cell walls by mild alkali treatments. Thioacidolysis analyses of cell walls from coleoptiles and various mesocotyl tissues demonstrated the presence of guaiacyl-, syringyl- and (traces of)p-hydroxyphenyl units besidesp-coumaric and ferulic acids. There are tissue-specific differences in amount and composition of lignins from different parts of the maize seedling. Electron-microscopic immunogold labeling of epitopes recognized by a specific anti-guaiacyl/syringyl antibody demonstrated the presence of lignin in all cell walls of the 4-d-old coleoptile. The primary walls of parenchyma and epidermis were more weakly labeled than the secondary wall thickenings of tracheary elements. No label was found in middle lamellae and cell corners. Lignin epitopes appeared first in the tracheary elements on day 2 and in the parenchyma on day 3 after sowing. Incubation of coleoptile segments in H2O2 increased the amount of extractable lignin and the abundance of lignin epitopes in the parenchyma cell walls. Lignin deposition was temporally and spatially correlated with the appearance of epitopes for prolinerich proteins, but not for hydroxyproline-rich proteins, in the cell walls. The lignin content of coleoptiles was increased by irradiating the seedlings with white or farred light, correlated with the inhibition of elongation growth, while growth promotion by auxin had no effect. It is concluded that wall stiffness, and thus extension growth, of the coleoptile can be controlled by lignification of the primary cell walls. Primary-wall lignin may represent part of an extended polysaccharide-polyphenol network that limits the extensibility of the cell walls.
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