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  • 1
    Keywords: Life sciences ; Microbiology ; Bacteriology ; Life sciences ; Microbiology ; Bacteriology ; Springer eBooks
    Description / Table of Contents: Gene Transfer: Transduction -- Gene Transfer: Transformation/Electroporation -- Gene transfer: Conjugation -- Pseudomonas Bacteriophage Isolation and Production -- Genotyping Methods -- Drug Susceptibility Testing by Dilution Methods -- Plate-based Assay for Swimming Motility in Pseudomonas aeruginosa -- Plate-based Assay for Swarming Motility in Pseudomonas aeruginosa -- Motility Assay: Twitching Motility -- Qualitative and Quantitative Assays for Flagellum-mediated Chemotaxis -- Microscopic Analysis: Morphotypes and Cellular Appendages -- Determination of Lipolytic Activities -- Elastinolytic and proteolytic enzymes -- In vitro Assays to Monitor the Activity of Pseudomonas aeruginosa Type III Secreted Proteins -- Cell Fractionation -- Characterization of Molecular Interactions using Isothermal Titration Calorimetry -- Proteomic Analysis -- Membrane Proteomics of Pseudomonas aeruginosa -- Construction of Pseudomonas Two-hybrid Libraries for High-throughput Assays -- Biosensors for Qualitative and Semi-quantitative Analysis of Quorum Sensing Signal Molecules -- LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules -- LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP -- Metabolic Footprinting: Extracellular Metabolomic Analysis -- Pyoverdine and Pyochelin Measurements -- Measurement of Phenazines in Bacterial Cultures -- Extraction and Measurement of NAD(P)+ and NAD(P)H -- Cyanide Measurements in Bacterial Culture and Sputum -- Monitoring Iron Uptake by Siderophores -- Exopolysaccharide Quantification -- Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids -- LPS quantitation Procedures -- Monitoring Lectin Interaction with Carbohydrates -- Mining the Pseudomonas Genome -- Identification of Bacterial Small RNAs by RNA Sequencing -- Gene Amplification and qRT-PCR -- The Standard European Vector architecture (SEVA) plasmid toolkit -- Chromosomal integration of transcriptional fusions -- A Method to Capture Large DNA Fragments from Genomic DNA -- Transposon Mutagenesis -- Site-directed Mutagenesis and Gene Deletion using Reverse Genetics -- Signature Tagged Mutagenesis -- Construction of a Pseudomonas aeruginosa Genomic Bank -- Strategy for Genome Sequencing Analysis and Assembly for Comparative Genomics of Pseudomonas Genomes -- Promoter Fusions with Optical Outputs in Individual Cells and in Populations -- Chromatin Immunoprecipitation for ChIP-chip and ChIP-Seq -- Transcriptional Analysis of Pseudomonas aeruginosa Infected Caenorhabditis elegans -- Methods for Studying Biofilm Formation: Flow Cells and Confocal Laser Scanning Microscop -- Biofilm Formation in the 96-well Microtiter Plate -- Methods for Studying Biofilm Dispersal in Pseudomonas aeruginosa -- Pseudomonas aeruginosa PA14 Pathogenesis in Caenorhabditis Elegans -- Assessing Pseudomonas Virulence using a Non-mammalian Host Model: Dictyostelium Discoideum -- Assessing Pseudomonas virulence with non-mammalian host: Galleria mellonella -- Assessing Pseudomonas Virulence with the Non-mammalian Host: Arabidopsis Thaliana -- Assessing Pseudomonas aeruginosa Persister/Antibiotic Tolerant Cells -- Assessing Pseudomonas Virulence with Non-mammalian Host: Zebrafish -- Assessing Pseudomonas Virulence with a Non-mammalian Host: Drosophila Melanogaster -- Assessing Pseudomonas Virulence using Host Cells -- Assessing Pseudomonas aeruginosa℗ Virulence and the Host Response using Murine Models of Acute and Chronic Lung Infection -- Assessing Pseudomonas Virulence using Mammalian Models: Acute Infection Model -- Burn Mouse Models
    Abstract: In℗ Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings
    Pages: XVIII, 816 p. 157 illus., 89 illus. in color. : online resource.
    ISBN: 9781493904730
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  • 2
    Keywords: Life sciences ; Biotechnology ; Cell Membranes ; Life sciences ; Membrane Biology ; Biotechnology ; Springer eBooks
    Abstract: This contributed volume reviews the recent progress in our understanding of membrane transport in yeast including both Saccharomyces cerevisiae and non-conventional yeasts. The articles provide a summary of the key transport processes and put these in a systems biology context of cellular regulation, signal reception and homeostasis. After a general introduction, readers will find review articles covering the mechanisms and regulation of transport for various substrates ranging from diverse nutrients to cations, water and protons. These articles are complemented by a chapter on extremophilic yeast, a chapter on the mathematical modelling of ion transport and two chapters on the role of transport in pathogenic yeasts and antifungal drug resistance. Each article provides both a general overview of the main transport characteristics of a specific substrate or group of substrates and the unique details that only an expert working in the field is able to transmit to the reader. Researchers and students of the topic will find this book to be a useful resource for membrane transport in yeast collecting information in one complete volume, which is otherwise scattered across many papers. This might also be interesting for scientists investigating other species in order to compare transport mechanisms with known functions in yeast with the cells on which they work
    Pages: VIII, 379 p. 45 illus., 25 illus. in color. : online resource.
    Edition: 1st ed. 2016.
    ISBN: 9783319253046
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  • 3
    Keywords: Medicine ; Microbiology ; Biomedicine ; Biomedicine general ; Microbiology ; Springer eBooks
    Description / Table of Contents: Pseudomonas aeruginosa Host Immune Evasion -- Pseudomonas entomophila: a versatile bacterium with entomopathogenic properties -- Pseudomonas Activation of the Inflammasome -- P. aeruginosa type VI secretion machinery: another deadly syringe -- Anaerobic life style of Pseudomonas aeruginosa -- Pseudomonas strains that exert biocontrol of plant pathogens -- Resistance and response to anti-pseudomonas agents and biocides -- Lessons from the QSCR structure for Quorum sensing -- Understanding and Exploiting Bacterial Outer Membrane Vesicles -- Events in root colonization by Pseudomonas putida -- My memories of Pseudomonas in the twentieth century
    Abstract: Pseudomonas volume 7 collects some of the most relevant and emerging issues in the biology of these microorganisms, and a number of other important issues that were not collected in the previous volumes. ℗ The first six volumes of the Pseudomonas series covered the biology of pseudomonads in a wide range of contexts, including the niches they inhabit, the taxonomic relations among its members of this group, the molecular biology of gene expression in different niches and under different environmental conditions, the analysis of virulence in plants, animal and human pathogens, as well as the determinants that make some of these strains of interesting for biotechnological applications. ℗ This seventh volume covers the following topics: The history of the biology of Pseudomonas The use of Pseudomonas as biological agents New trends in the molecular biology of these microorganisms Pseudomonas and the immune system of insects and animals ℗ This book will be of use to researchers working on these bacteria, particularly those studying medical aspects of Pseudomonas, and their use as a means to control pathogens or to stimulate plant growth. This volume is also interesting for those studying the physiology, genetics, molecular biology of Pseudomonas and those using novel-omics approaches to understand bacteria of the genus Pseudomonas
    Pages: XII, 316 p. 40 illus. in color. : online resource.
    ISBN: 9789401795555
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  • 4
    Keywords: Life sciences ; Gene Expression ; Medical genetics ; Microbiology ; Bacteriology ; Microbial genetics ; Life sciences ; Bacteriology ; Microbial Genetics and Genomics ; Medical Microbiology ; Gene Expression ; Gene Function ; Springer eBooks
    Pages: : digital
    ISBN: 9789048139095
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  • 5
    Keywords: Medicine ; Immunology ; Toxicology ; Internal Medicine ; Rheumatology ; Biomedicine ; Pharmacology/Toxicology ; Immunology ; Rheumatology ; Internal Medicine ; Springer eBooks
    Description / Table of Contents: Historic outline of the development of drugs targeting B-cells -- Understanding B-cell biology -- Pharmacological effects and mechanisms of action of agents blocking B cells -- Systemic lupus erythematosus: direct B-cell blocking -- Systemic lupus erythematosus: indirect B-cell blocking -- B-cell targeted therapies in rheumatoid arthritis -- B-cell targeted therapies in Primary Sjögren Syndrome -- Targeting B cells in ANCA-associated vasculitis -- B-cell targeted therapies in systemic sclerosis and inflammatory myopathies -- B-cell targeted therapies in cryoglobulinemia -- B-cell targeted therapies in autoimmune cytopenias and thrombosis -- Targeting B cells in neurological autoimmune diseases -- Targeting B cells in other systemic autoimmune diseases -- Safety of B-cell targeted therapies -- The future potential of biosimilars targeting B-cells
    Abstract: This book provides a detailed overview of B-cell directed therapies in patients with rheumatic and systemic autoimmune diseases, including rheumatoid arthritis, systemic lupus erythematosus, Sjögren syndrome, ANCA-associated vasculitis and cryoglobulinemia. Organ-specific autoimmune diseases are discussed with respect to the use of B-cell directed therapies in neurological autoimmune diseases and autoimmune cytopenias. Situations in which B-cell targeted therapy may be indicated are identified, thereby offering comprehensive support for therapeutic decisions on the basis of the latest published evidence. The book also offers a valuable reference tool for rheumatologists, internists, nephrologists, immunologists, and all specialists involved in the multidisciplinary care of patients with rheumatic and systemic autoimmune diseases
    Pages: XI, 292 p. 3 illus., 2 illus. in color. : online resource.
    ISBN: 9783034807067
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  • 6
    Keywords: Medicine ; Internal Medicine ; Rheumatology ; Medicine & Public Health ; Rheumatology ; Internal Medicine ; Springer eBooks
    Pages: : digital
    ISBN: 9780857293589
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  • 7
    Keywords: Medicine ; Family medicine ; Internal Medicine ; Ophthalmology ; Rheumatology ; Medicine & Public Health ; Rheumatology ; Internal Medicine ; General Practice / Family Medicine ; Ophthalmology ; Springer eBooks
    Pages: : digital
    ISBN: 9780857299475
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  • 8
    ISSN: 1432-0711
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A controlled crossover clinical comparison of 40-g/day and 80-g/day vegetable protein diets vs a 40g/day meat protein diet plus neomycin-milk of magnesia (as control therapy) was performed on 10 cirrhotic patients with mild chronic portal-systemic encephalopathy. The 40-g vegetable protein diet had a high fiber volume and contained low methionine and low aromatic amino acids. The 80-g vegetable protein diet was rich in branched-chain amino acids and fiber, with a similar content of sulfur-containing amino acids as compared to the 40-g meat protein diet. Serial semiquantitative assessments were done, including mental state, asterixis, number connection tests electroencephalograms and blood ammonia levels. No patient developed deep coma while ingesting either vegetable protein diet or neomycin-milk of magnesia plus 40-g meat protein diet. A significant improvement in the number connection test times was observed during the 40-g vegetable protein diet (P〈0.05) and during the 80-g vegetable protein diet (P〈0.05) as compared to their previous 40-g meat protein-neomycin periods. In addition, during the period of 80-g vegetable protein diet, the patients showed a significant improvement in their electroencephalograms (P〈0.05). The frequency of bowel movements significantly increased (P〈0.05) during the 80-g vegetable protein diet period. During the 40-g vegetable protein diet, two cirrhotic-diabetic patients experienced hypoglycemia. Three patients complained of the voluminous 80-g vegetable protein diet. Patients with mild portal-systemic encephalopathy may be adequately controlled with vegetable protein diets as a single therapy.
    Type of Medium: Electronic Resource
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  • 10
    Publication Date: 2018-03-06
    Description: Skin aging is a gradual process that leads to wrinkle formation, laxity, and overall changes in skin appearance. In recent years, the demands to noninvasive treatments for facial rejuvenation increased, along with a variety of technologies and devices, such as radiofrequency. The present study aimed to evaluate the clinical effects of a multipolar radiofrequency and pulsed electromagnetic field treatment for face and neck rejuvenation. Eleven patients with mild to moderate grades of photoaging underwent eight radiofrequency and pulsed electromagnetic field treatment sessions, once a week. Clinical photographs were taken before and a week after the end of the treatment, and improvement of facial skin parameters was evaluated by two different investigators. Significant improvement in skin laxity was observed in all eleven patients (100%). Improvement in facial contour was noted in 73% and 100% of patients when analyzed by investigators A and B, respectively. The score for overall improvement in skin condition was 3 ± 0.78 for investigator A and 3.6 ± 0.67 for investigator B. All patients were satisfied with the procedure and noted significant improvement in the skin. The combined multipolar radiofrequency and pulsed electromagnetic field device is effective and safe for treatment of aged skin in Brazilian patients.
    Print ISSN: 1687-6105
    Electronic ISSN: 1687-6113
    Topics: Medicine
    Published by Hindawi
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