ISSN:
1420-9071
Keywords:
Bottleneck theory
;
calorimetry
;
chemostat
;
Crabtree effect
;
glucose effect
;
S. cerevisiae
;
yeast metabolism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The growth physiology ofSaccharomyces cerevisiae strains H1022 and Whi2+ has been studied in aerobic batch and continuous (chemostat) cultures. Results from the measurement of biomass and medium components (off-line) together with oxygen, carbon dioxide and heat measurements (on-line) have been used in an attempt to explore the existence of ‘overflow’ or ‘bottleneck’ metabolism as opposed to catabolite repression (Crabtree effect) in these strains. Chemostat experiments indicated that specific oxygen uptake rate (q o 2) was linearly related to the dilution rate (D) at values below the critical dilution rate (D crit ), becoming constant aboveD crit , which is in agreement with the bottleneck theory. However, batch culture experiments indicated negligible oxygen consumption during the initial glucose growth phase, the culture exhibiting purely anaerobic metabolism. The bottleneck theory would propose thatq O 2, has a constant (maximum) value under these conditions. The results presented here suggest that while the bottleneck theory can be adequately used to describe chemostat growth ofS. cerevisiae, some other control mechanism must be operating under conditions of high glucose concentrations, such as those initially prevailing in the batch culture experiments.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01950151
Permalink
