Description / Table of Contents:
Negative And Positive Separation Techniques For The Isolation Of Antigen-Specific Cd8+ T Cells From Blood And Tumor Tissue -- In Vitro\Ex Vivo Generation Of Cytotoxic T Lymphocytes -- Phenotypic And Functional Characterization Of Cytotoxic T Lymphocytes By Flow Cytometry -- ℗ Intracellular Signalling Of Ctls -- Monitoring Antigen-Specific T Cell Responses Using Real-Time Pcr -- Ctl Elispot Assay -- Methods Of Purification Of Ctl-Derived Exosomes -- Characterization Of Ctl By Microscopy -- Intravital Imaging Of Cytotoxic T Lymphocytes -- The Microarray-Based Approach For The Analysis Of The Transcriptome -- Mirnome Analysis Using Real Time Pcr -- Proteomic Approaches By Seldi And Maldi-Tof/Ms For Ctl Analysis -- Two-Dimensional Gel Electrophoresis Approach For Ctl Phosphoproteome Analysis -- Targeting ℗ The Jak/Stat Pathway In Cytotoxic T Lymphocytes (Ctl) By Next Generation Sequencing (Ngs) -- Experimental Model For Studying The Involvement Of Regulatory Cytotoxic T Cells In Bone Resorption -- Ctl And Transplantation: Tissue In Vivo Characterization
℗ In Cytotoxic T-Cells: Methods and Protocols, leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies. ℗ The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation. ℗ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Cytotoxic T-Cells: Methods and Protocols seeks to aid scientists in the further study into concepts of laboratory methods using systems biology
XII, 301 p. 32 illus., 26 illus. in color. : online resource.