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  • 1
    Keywords: Life sciences ; Human Genetics ; Animal genetics ; Life sciences ; Animal Genetics and Genomics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Twenty Years of qPCR: A Mature Technology? -- Minimum Information Necessary for Quantitative Real-Time PCR Experiments -- Selection of Reliable Reference Genes for RT-qPCR Analysis -- Introduction to Digital PCR -- mRNA and micro RNA Purity and Integrity: The Key to Success in Expression Profiling -- Mediator Probe PCR: Detection of Real-Time PCR by Label Free Probes and a Universal Fluorogenic Reporter -- Absolute Quantification of Viral DNA: The Quest for Perfection -- A Multiplex Real-Time PCR-Platform Integrated into Automated Extraction Method for the Rapid Detection and Measurement of Oncogenic HPV Type-Specific Viral DNA Load from Cervical℗ Samples -- Real-Time PCR Detection of Mycoplasma pneumoniae in the Diagnosis of℗ Community-Acquired Pneumonia -- A Sensible Technique to Detect Mollicutes Impurities in Human Cells Cultured in GMP Condition -- Real-Time Quantification Assay to Monitor BCR-ABL1 Transcripts in Chronic Myeloid Leukemia -- A Reliable Assay for Rapidly Defining Transplacental Metastasis Using Quantitative PCR -- Circulating Cell-Free DNA in Cancer -- Gene Expression Analysis by qPCR in Clinical Kidney Transplantation -- Post-Transcriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA and Micro RNA Data -- Clinical Applications Using Digital PCR -- Developing Non-Invasive Diagnosis for Single Gene Disorders: The Role of Digital PCR
    Abstract: Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation
    Pages: XI, 231 p. 80 illus., 66 illus. in color. : online resource.
    ISBN: 9781493907335
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  • 2
    Keywords: Genetics ; Human Genetics ; Genetics and Genomics ; Human Genetics ; Springer eBooks
    Description / Table of Contents: A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers -- Parameters for Successful PCR Primer Design -- MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing -- Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation -- A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR -- Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR) -- Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR) -- Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine -- A General Protocol for Accurate Gene Expression Analysis in Plants -- Gene Expression Analysis in Bacteria by RT-qPCR -- Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR -- Molecular Monitoring of Chronic Myeloid Leukemia -- Normalization in Human Gliomas Tissue -- qPCR Applications for the Determination of the Biological Age -- QuantStudio™ 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis -- Digital PCR and the QuantStudio™ 3D Digital PCR System
    Abstract: This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study
    Pages: XIII, 235 p. 70 illus., 55 illus. in color. : online resource.
    Edition: 2nd ed. 2020.
    ISBN: 9781493998333
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  • 3
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1588-2837
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Abstract Проведены предварительные исследования механизма реакции присоединения Михаэля этилацетоацетата к халкону. При различных условиях различные продукты были получены с хорошим выходом (≧80%) и селективностью (⋟100%). Предложенный механизм основан на геометрических характеристиках активных центров активированной гидроокиси бария (C-200).
    Notes: Abstract Preliminary studies of the mechanism of Michael addition of ethyl acetoacetate to chalcone are presented. Different products may be obtained with good yields (≧80%) and selectivities (≃100%), under different reaction conditions. A mechanism based on the geometric characteristics of the active site of activated barium hydroxide (C-200) is proposed.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 7
    Keywords: MORTALITY ; RISK ; GENE ; MICE ; ASSOCIATION ; BLOOD-PRESSURE ; HYPERTENSION ; CARDIOVASCULAR-DISEASE ; METAANALYSIS ; HEART-FAILURE ; ADRENERGIC-RECEPTOR TRAFFICKING
    Abstract: Numerous genetic loci have been associated with systolic blood pressure (SBP) and diastolic blood pressure (DBP) in Europeans(1-3). We now report genome-wide association studies of pulse pressure (PP) and mean arterial pressure (MAP). In discovery (N = 74,064) and follow-up studies (N = 48,607), we identified at genome-wide significance (P = 2.7 x 10(-8) to P = 2.3 x 10(-13)) four new PP loci (at 4q12 near CHIC2, 7q22.3 near PIK3CG, 8q24.12 in NOV and 11q24.3 near ADAMTS8), two new MAP loci (3p21.31 in MAP4 and 10q25.3 near ADRB1) and one locus associated with both of these traits (2q24.3 near FIGN) that has also recently been associated with SBP in east Asians. For three of the new PP loci, the estimated effect for SBP was opposite of that for DBP, in contrast to the majority of common SBP- and DBP-associated variants, which show concordant effects on both traits. These findings suggest new genetic pathways underlying blood pressure variation, some of which may differentially influence SBP and DBP
    Type of Publication: Journal article published
    PubMed ID: 21909110
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  • 8
    Keywords: RECEPTOR ; T-CELL ; PROMOTER ; BLOOD-PRESSURE ; protein expression ; GENOME-WIDE ASSOCIATION ; INDEPENDENT PREDICTOR ; ARTERIAL STIFFNESS ; AORTIC STIFFNESS ; HYPERTENSIVE PATIENTS
    Abstract: BACKGROUND: Carotid-femoral pulse wave velocity (CFPWV) is a heritable measure of aortic stiffness that is strongly associated with increased risk for major cardiovascular disease events. METHODS AND RESULTS: We conducted a meta-analysis of genome-wide association data in 9 community-based European ancestry cohorts consisting of 20 634 participants. Results were replicated in 2 additional European ancestry cohorts involving 5306 participants. Based on a preliminary analysis of 6 cohorts, we identified a locus on chromosome 14 in the 3'-BCL11B gene desert that is associated with CFPWV (rs7152623, minor allele frequency=0.42, beta=-0.075+/-0.012 SD/allele, P=2.8x10(-10); replication beta=-0.086+/-0.020 SD/allele, P=1.4x10(-6)). Combined results for rs7152623 from 11 cohorts gave beta=-0.076+/-0.010 SD/allele, P=3.1x10(-15). The association persisted when adjusted for mean arterial pressure (beta=-0.060+/-0.009 SD/allele, P=1.0x10(-11)). Results were consistent in younger (〈55 years, 6 cohorts, n=13 914, beta=-0.081+/-0.014 SD/allele, P=2.3x10(-9)) and older (9 cohorts, n=12 026, beta=-0.061+/-0.014 SD/allele, P=9.4x10(-6)) participants. In separate meta-analyses, the locus was associated with increased risk for coronary artery disease (hazard ratio=1.05; confidence interval=1.02-1.08; P=0.0013) and heart failure (hazard ratio=1.10, CI=1.03-1.16, P=0.004). CONCLUSIONS: Common genetic variation in a locus in the BCL11B gene desert that is thought to harbor 1 or more gene enhancers is associated with higher CFPWV and increased risk for cardiovascular disease. Elucidation of the role this novel locus plays in aortic stiffness may facilitate development of therapeutic interventions that limit aortic stiffening and related cardiovascular disease events.
    Type of Publication: Journal article published
    PubMed ID: 22068335
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  • 9
    Abstract: Pilocytic astrocytoma and ganglioglioma may occur in inaccessible or surgically difficult areas. In case of incomplete resection, the availability of biological predictors of tumour progression could be particularly important. To this end, an analysis of p53 codon 72 polymorphism and assessment of its role as prognostic marker were performed.The status of the p53 Arg72Pro polymorphism was evaluated by pyrosequencing method in a multicenter cohort of 170 paediatric patients. Genotype/phenotype associations were investigated either by means of bivariate or multivariate analyses.In the partially resected pilocytic astrocytomas, the Arg/Arg variant predicts early tumour progression (median survival time: 23.1 months) and is associated with poor event-free survival (p value = 0.0009). This finding remains true also in case of adjuvant therapies, with a 5-year event-free survival of 30.6% for cases with Arg/Arg variant vs. 78.7% for those with other genotypes. There is no association between ganglioglioma and the polymorphism.The assessment of Arg/Arg variant could improve the management of pilocytic astrocytoma. TP53 codon 72 analysis could distinguish low-risk cases, in which surgery could be conservative, from high-risk cases needing an aggressive surgery plan.
    Type of Publication: Journal article published
    PubMed ID: 27374106
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  • 10
    Keywords: APOPTOSIS ; CELLS ; EXPRESSION ; IN-VITRO ; INHIBITOR ; INVASION ; SURVIVAL ; tumor ; CELL ; human ; INHIBITION ; VITRO ; COMMON ; RISK ; GENE ; GENES ; PROTEIN ; cell line ; TUMORS ; LINES ; murine ; primary ; SKIN ; CELL-LINES ; PHOSPHORYLATION ; SIGNAL ; FORM ; IDENTIFICATION ; PROGRESSION ; INDUCED APOPTOSIS ; METASTASIS ; CELL-LINE ; LINE ; MODULATION ; MELANOMA ; LOCALIZATION ; A431 CELLS ; PHENOTYPE ; INTEGRIN ; skin tumors ; PROJECT ; protein expression ; INHIBITORS ; CHAIN ; XENOGRAFTS ; HUMAN-MELANOMA ; 12(S)-HETE ; human melanoma ; platelet-type12-lipoxygenase
    Abstract: As previous studies suggested the expression of a 12-LOX enzyme in murine and human melanoma cell lines, the primary aim of this project was to genetically identify the 12-LOX enzyme (platelet-, leukocyte- or epithelial form). By using reverse transcriptase-polymerase chain reaction, sequencing and various immunological techniques we have demonstrated conclusively the expression of the platelet-type 12-LOX in human melanoma cells of different origin, in their transplanted xenografts and in fresh human skin tumors. Furthermore, we found that p12-LOX is able to provide a survival signal for melanoma cells since inhibition of the enzyme by general LOX or selective 12-LOX inhibitors induced apoptosis in vitro. p12-LOX of human melanoma has been shown to be involved in the control of the metastatic phenotype, since we have detected the upregulation of the 12-LOX protein expression in spontaneously metastasizing xenografts and in thick human skin tumors (〉 3.0 mm) characterized by high risk for the development of metastasis. Co-expression of two megakaryocytic genes, p12-LOX and alphaIIb integrin chains, was found to be a frequent phenomenon in human melanoma (approximately 70%) suggesting a common regulatory defect in this tumor. (C) 2004 Lippincott Williams Wilkins
    Type of Publication: Journal article published
    PubMed ID: 15305153
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