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  • 1
    Unknown
    New York : Wiley.
    Call number: ATV-CYT:138/1
    Keywords: Cytology / Laboratory manuals ; Flow cytometry / Laboratory manuals ; Cell separation / Laboratory manuals ; Molecular biology / Laboratory manuals
    Notes: Not kept up to date.
    Pages: loose-leaf
    ISBN: 0471161314
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    ATV-CYT:138/1 departmental collection or stack – please contact the library
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  • 2
    Unknown
    New York : Wiley
    Call number: ATV-CYT:138/2
    Keywords: Cytology / Laboratory manuals ; Flow cytometry / Laboratory manuals ; Cell separation / Laboratory manuals ; Molecular biology / Laboratory manuals
    Notes: Not kept up to date.
    Pages: loose-leaf
    ISBN: 0471161314
    Signatur Availability
    ATV-CYT:138/2 departmental collection or stack – please contact the library
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  • 3
    ISSN: 0303-7207
    Keywords: cholesterol side-chain cleavage ; corpus luteum ; gonadotropin ; steroidogenesis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1041
    Keywords: chlordiazepoxide ; alcoholic liver disease ; pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The clearance of chlordiazepoxide from the systemic circulation was studied in 20 subjects which included 15 patients with alcoholic hepatitis and 5 normal volunteers. The half-life for the appearance of the drug in the systemic circulation was found to increase exponentially with age (r=0.73, P〈0.0005) and was independent of the presence of alcoholic hepatitis. The metabolic clearance of chlordiazepoxide was significantly lower in the patients than in the normal subjects (7.6 compared to 13.8 ml/kg-h, P〈0.005). Linear regression analysis revealed a significant correlation between clearance and albumin (r=0.77, P〈0.00005). However, the predictive value of this relationship was shown to be minimal. Multiple regression analysis produced only a slight improvement in the correlation when both albumin and lactate dehydrogenase were used as variables (r=0.83, P〈0.00005). In six of the patients, a second clearance study was conducted three weeks following their initial one. All repeat subjects showed improvement both clinically and as reflected by their laboratory tests for liver injury, but there was not a significant change in their clearance of chlordiazepoxide. Multiple regression analysis of the clearance data on the initial and repeat subjects showed a significant correlation between clearance and the variables age, albumin, and lactate dehydrogenase (r=0.91, P〈0.0025). This relationship suggests that over a short period of time (where age can be considered constant) changes in albumin and lactate dehydrogenase could be potentially useful in predicting clearance changes in a single individual.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1106
    Keywords: Nucleus raphe magnus ; Spinal trigeminal nucleus ; Spinal cord ; Retrograde transport ; Fluorescent dyes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Retrograde transport of the fluorescent dyes Evans blue and 4,6-diamidino-2-phenyl indole (DAPI) has been used to study projections from the medullary raphe nuclei to the trigeminal nucleus caudalis and to the dorsolateral quadrant of the thoracolumbar cord in the rat. The majority of projecting neurones were found in n. raphe magnus (NRM) and its ventrolateral extensions over the pyramids and inferior olive. Double labelling experiments showed that 94% of raphe-trigeminal neurones sent a collateral branch to the nucleus in the contralateral brainstem. Similarly, 92% of raphetrigeminal neurones branched to supply the thoracolumbar cord. It is suggested that the widespread nature of the analgesia produced by electrical stimulation in NRM in conscious animals may be due to activation of a population of raphe neurones which inhibit the responses of neurones in the trigeminal nucleus and dorsal horn via an extensive system of collateral projections to these structures.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Normal skeletal and cardiac striated muscle from adult rats was incubated for the cytochemical detection of acid phosphatase activity with cerium as the capture metal. Results from these experiments show that normal striated muscle has a greater number of acid phosphatase-positive structures, which are presumed to be lysosomes, than has been indicated by several previous cytochemical studies.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Phagocytosis by polymorphonuclear leukocytes triggers a burst of oxidative metabolism resulting in hydrogen peroxide and superoxide production, and these active oxygen species function in the killing of microorganisms. A new cytochemical technique, based on a manganese dependent diaminobenzidine oxidation, has been developed to detect superoxide in these cells. It has been shown that superoxide generation is associated with the plasma membrane in cells activated by particulate (zymosan) and nonparticulate (phorbol myristate acetate) stimuli. This membraned activity is maintained during invagination such that reduced oxygen is generated within the endocytic vacuoles. Reaction product is absent from unstimulated cells; additionally, formation of precipitate is blocked by omission of Mn++, low temperature, glutaraldehyde prefixation, and the presence of superoxide dismutase in the incubation medium.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-1076
    Keywords: Key words Acute otitis media ; Ceftriaxone ; Amoxicillin clavulanate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two hundred and fifteen children aged 4 months–6 years with acute otitis media (AOM) were randomized to be treated either by a single i.m. injection of ceftriaxone, 50 mg/kg, with a second dose in the event of unsatisfactory response after 48 h or a history of recurrent AOM (109 patients) or amoxicillin clavulanate 12.5 mg tid (106 patients). The failure rate was similar in children treated by ceftriaxone and amoxicillin clavulanate, 4.6% and 4.7%, respectively (standard error for intergroup difference −2.87%, 95% confidence interval −5.62% to 5.87%). No significant differences between the groups were found in the dynamics of the resolution of the acute symptomatology, otoscopy findings, relapse rate at 30 days or tympanographic evidence of middle ear effusion at the scheduled visits on days 30, 60 and 90. Recurrence of AOM between days 31 and 90 was observed significantly in more children treated with amoxicillin clavulanate than with ceftriaxone – 25 out of 84 (29.4%) versus 11 out of 81 (13.6%) (P=0.012). Conclusion Ceftriaxone injection(s) is as efficient at least as 10-day oral amoxicillin clavulanate for treatment of acute otitis media in children. Although not recommended as routine, ceftriaxone can be considered in the management of acute otitis media under special circumstances, particularly in cases when the ability to tolerate or absorb oral drugs is compromised, in children refusing or unable to take oral therapy or when the compliance is questionable.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1106
    Keywords: Electrophysiology ; Dentate granule cells ; Behavioral correlates ; Sensory discrimination learning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The activity of dentate granule cells (g-cells) was recorded in chronic rats during single-tone sensory discrimination performance. Attempts to correlate the discharge pattern of dentate g-cells with (1) the onset of the conditioned tone stimulus, (2) movements of the animal during the conditioning trials, (3) the occurrence of the reinforced response, and (4) the presence or absence of theta rhythm during the trial revealed several features of g-cell discharge during performance of operant sensory discrimination. The most consistent finding was that g-cells were driven at short latencies (40–60 ms) by the onset of the tone stimulus in the absence of detectable body movements. A subsequent, less intense sustained discharge of g-cells was shown to be unrelated to the onset of (1) conditioned movements during the trial, or (2) the execution of the conditioned response. Thus neither phase of g-cell discharge to the tone stimulus appeared to be a direct correlate of inadvertently or directly conditioned movements in this experimental paradigm. Theta rhythm, which accompanied certain movements prior to the execution of the operant response, did not correlate with the initial phase of g-cell discharge but appeared to modulate the subsequent phase of sustained g-cell activity.
    Type of Medium: Electronic Resource
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