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  • 1
    ISSN: 1432-0983
    Keywords: Linear mitochondrial plasmid ; Nucleotide sequence ; Viral DNA-polymerase ; Terminal inverted repeat (TIR) ; Adenovirus ; Morchella conica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary pMC3-2, one of two linear plasmids localised in the mitochondria of the ascomycete Morchella conica, was completely sequenced. It is 6044 bp in size, contains terminal inverted repeats of 713 and 710 bp length and two open reading frames, ORF1 and ORF2, spanning 2706 bp and 918 bp, respectively. ORF1 probably encodes a viral B-type DNA-polymerase. Concerning ORF2, no homology to any other published protein-or DNA-sequence could be detected. According to the structure of DNA-polymerases, linear plasmids can be grouped into two classes reflecting their localisation either in the cytoplasm or within the mitochondria. In general, the structure of plasmid pMC3-2, as well as of other linear plasmids from filamentous fungi, indicates a close relationship of these genetic elements to adenoviruses.
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  • 2
    ISSN: 1432-0983
    Keywords: Linear plasmids ; Phylogenetic trees
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Relative phylogenetic distances were estimated for those linear plasmids for which sequencing data were available by comparing the amino-acid sequences of the putative DNA- and RNA-polymerases, and phylogenetic trees were calculated. The relationships obtained accord well with those indicated by other structural characteristics of these genetic elements. It is obvious that linear plasmids constitute a separate group of genetic traits when compared with those of the adenoviruses. However, an overall relationship to these viruses is evident. Among the linear plasmids at least two main groups can be recognized, namely the cytoplasmically and the mitochondrially localized elements.
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  • 3
    ISSN: 1432-0983
    Keywords: Linear plasmids ; Morchella conica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitochondrial RNA was isolated from the morel strain Morchella conica 3 harbouring the linear plasmid pMC3-2 and subjected to gel electrophoresis followed by a Northern analysis using cloned fragments of the plasmid pMC3-2 as probes. Hybridization was obtained only with central parts of pMC3-2 and specific bands of mtRNA. The hybridization bands (2.8 kb and 1.0 kb) correspond in size to the length of the two ORFs of pMC3-2 which were deduced from nucleotide-sequence data. Thus, both ORFs, one encoding a DNA polymerase and the other a yet unknown protein, are transcribed in the mitochondria of the plasmid-bearing Morchella conica strain.
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  • 4
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 5
    ISSN: 1432-0983
    Keywords: Key words  Hygromycin ; Phleomycin ; GUS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   The barley leaf scald fungus, Rhynchosporium secalis, was transformed to hygromycin-B and phleomycin resistance using the hph gene from E. coli and the ble gene from Streptoalloteichus hindustanus under the control of Aspergillus nidulans promoter and terminator sequences. Plasmid DNA was introduced into fungal protoplasts by PEG/CaCl2 treatment. Transformation frequencies varied from 59 to 493 transformants per 10 µg of DNA and 5 × 107 protoplasts. The antibiotic-resistant phenotype appeared to be stable under selective, as well as under non-selective, conditions for several generations. Co-transformation using the E. coli uidA gene under the control of A. nidulans promoter and terminator sequences on a non-selectable plasmid occurred at frequencies of up to 66%.
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  • 6
    ISSN: 1432-0983
    Keywords: Hygromycin ; Phleomycin ; GUS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The barley leaf scald fungus,Rhynchosporium secalis, was transformed to hygromycin-B and phleomycin resistance using thehph gene fromE. coli and theble gene fromStreptoalloteichus hindustanus under the control ofAspergillus nidulans promoter and terminator sequences. Plasmid DNA was introduced into fungal protoplasts by PEG/CaCl2 treatment. Transformation frequencies varied from 59 to 493 transformants per 10 μg of DNA and 5x107 protoplasts. The antibiotic-resistant phenotype appeared to be stable under selective, as well as under nonselective, conditions for several generations. Co-transformation using theE. coli uidA gene under the control ofA. nidulans promoter and terminator sequences on a non-selectable plasmid occurred at frequencies of up to 66%.
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  • 7
    ISSN: 1434-3916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird ein Verfahren der offenen Muskelbiopsie beschrieben, das für den Patienten wenig invasiv and für das Muskelgewebe atraumatisch ist und unter Lokalanästhesie durchgeführt wird. Die Muskelproben können aus einem definierten Bereich entnommen werden and gestatten die morphologisch-quantitative Auswertung eines hinreichend großen Materials, um zu zuverlässigen Ergebnissen zu gelangen. Die Vorteile der Methode gegenüber anderen Verfahren der offenen und Nadelbiopsie, insbesondere das Anästhesieverfahren, werden diskutiert.
    Notes: Summary A method of open muscle biopsy is described which is not so invasive for the patient and not traumatic for the muscle tissue. The biopsy is performed with the patient under local cutaneous anesthesia. The sample can be obtained from a defined part of the muscle. It allows morphological-quantitative evaluation of a sufficient amount of fibers to arrive at reliable results. The advantages of the described method compared with other open and needle biopsy procedures, especially with regard to the method of anesthesia, are discussed.
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  • 8
    ISSN: 1437-160X
    Keywords: Neopterin ; Arthritis ; Synovial fluids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Neopterin levels of serum and fluid were determined in 30 patients with inflammatory synovial fluids and in 30 controls with joint effusions induced by trauma or osteoarthritis. Neopterin was significantly elevated in inflammatory fluids. There was no correlation with the acute phase reactants. Neopterin levels were significantly higher in inflammatory fluids than in serum, and vice versa in non-inflammatory conditions. Our results indicate that in arthritis neopterin is produced locally in the joint by monocytes induced by T-cell activation.
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