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  • 1
    Publication Date: 2013-06-04
    Description: DNA damage activates a signalling network that blocks cell-cycle progression, recruits DNA repair factors and/or triggers senescence or programmed cell death. Alterations in chromatin structure are implicated in the initiation and propagation of the DNA damage response. Here we further investigate the role of chromatin structure in the DNA damage response by monitoring ionizing-radiation-induced signalling and response events with a high-content multiplex RNA-mediated interference screen of chromatin-modifying and -interacting genes. We discover that an isoform of Brd4, a bromodomain and extra-terminal (BET) family member, functions as an endogenous inhibitor of DNA damage response signalling by recruiting the condensin II chromatin remodelling complex to acetylated histones through bromodomain interactions. Loss of this isoform results in relaxed chromatin structure, rapid cell-cycle checkpoint recovery and enhanced survival after irradiation, whereas functional gain of this isoform compacted chromatin, attenuated DNA damage response signalling and enhanced radiation-induced lethality. These data implicate Brd4, previously known for its role in transcriptional control, as an insulator of chromatin that can modulate the signalling response to DNA damage.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683358/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683358/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Floyd, Scott R -- Pacold, Michael E -- Huang, Qiuying -- Clarke, Scott M -- Lam, Fred C -- Cannell, Ian G -- Bryson, Bryan D -- Rameseder, Jonathan -- Lee, Michael J -- Blake, Emily J -- Fydrych, Anna -- Ho, Richard -- Greenberger, Benjamin A -- Chen, Grace C -- Maffa, Amanda -- Del Rosario, Amanda M -- Root, David E -- Carpenter, Anne E -- Hahn, William C -- Sabatini, David M -- Chen, Clark C -- White, Forest M -- Bradner, James E -- Yaffe, Michael B -- 1-U54-CA112967-04/CA/NCI NIH HHS/ -- ES-002109/ES/NIEHS NIH HHS/ -- P30 CA014051/CA/NCI NIH HHS/ -- P30 ES002109/ES/NIEHS NIH HHS/ -- P30-CA14051/CA/NCI NIH HHS/ -- R01 ES015339/ES/NIEHS NIH HHS/ -- R01-ES15339/ES/NIEHS NIH HHS/ -- R21 CA109661/CA/NCI NIH HHS/ -- R21 NS063917/NS/NINDS NIH HHS/ -- R21-NS063917/NS/NINDS NIH HHS/ -- U54 CA112967/CA/NCI NIH HHS/ -- England -- Nature. 2013 Jun 13;498(7453):246-50. doi: 10.1038/nature12147. Epub 2013 Jun 2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23728299" target="_blank"〉PubMed〈/a〉
    Keywords: Acetylation ; Adenosine Triphosphatases/metabolism ; Cell Cycle Checkpoints/radiation effects ; Cell Line, Tumor ; Cell Survival/radiation effects ; Chromatin/chemistry/*metabolism/radiation effects ; *Chromatin Assembly and Disassembly/radiation effects ; *DNA Damage ; DNA Repair/radiation effects ; DNA-Binding Proteins/metabolism ; Histones/chemistry/metabolism ; Humans ; Lysine/chemistry/metabolism ; Multiprotein Complexes/metabolism ; Nuclear Proteins/chemistry/deficiency/genetics/*metabolism ; Phosphorylation/radiation effects ; Positive Transcriptional Elongation Factor B/metabolism ; Protein Isoforms/metabolism ; Radiation, Ionizing ; *Signal Transduction/radiation effects ; Transcription Factors/chemistry/deficiency/genetics/*metabolism
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 213 (1967), S. 504-505 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A bioassay of the culture filtrate using Botrytis allii as the test organism showed the very characteristic effects of griseofulvin2. The presence of this antibiotic was confirmed by its isolation in low yields from both extracts as colourless needles, identity with authentic material being ...
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 89 (2001), S. 6530-6535 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The dynamic soft response of polydomain liquid crystalline elastomers to simple shear is reported. Significantly, these materials also show extremely large loss behavior with tan δ exceeding 1 or even 1.5 over very wide temperature ranges, with clear implications for damping applications. By comparing materials that exhibit different types of liquid crystalline phases, we identify the nematic state as a better damping phase than that in materials with smectic phases. Additionally, we provide experimental evidence for directions which should be explored for further improvements in the damping behavior of liquid crystalline elastomers. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Clinical Anatomy 6 (1993), S. 94-105 
    ISSN: 0897-3806
    Keywords: arteries ; thigh vessels ; femoral artery ; profunda femoris ; Life and Medical Sciences ; Miscellaneous Medical
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The medial femoral circumflex artery (MFC) is important in its supply to the head and neck of the femur and to the adductor and hamstring muscles, several of which, notably the gracilis and biceps femoris, are used commonly in reconstructive surgical procedures. Considering the clinical significance of this artery, it is somewhat surprising to note the present lack of clarity of description and inconsistency of the nomenclature for this vessel and its branches, as seen in respected sources. This project was undertaken 1) to reexamine the origin, course, and distribution of the MFC and 2) to reevaluate the nomenclature in the anatomic and clinical literature for this artery and its branches. In 30 cadaveric specimens the MFC arose most commonly from the deep femoral artery (53%) or the femoral artery (40%). The MFC typically gave origin to two major ventral muscular branches and a small trochanteric branch before passing from the adductor compartment into the flexor compartment of the thigh. Thereafter, the MFC divided into an ascending branch to the hip and a descending branch to the hamstrings and sciatic nerve. We recommend the use of the terms “ascending” and “descending,” which are listed in the 6th Edition of the Nomina Anatomica, and the deletion of “transverse,” “superficial,” and “deep” as named branches of the MFC. We question the description of the “crucial” or “cruciate anastomosis,” as usually taught to medical students. © 1993 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1022-1352
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: We report on the structural study of a non-uniform (polydomain) director texture in nematic and smectic liquid crystalline elastomers. Polarised light scattering is used to probe the equilibrium director correlations in the region of transition under stress between the opaque polydomain material and the transparent, macroscopically monodomain elastomer. A characteristic four-peak scattering image is obtained in this transient region. The lobes of intensity are oriented along and perpendicular to the axes of crossed polarisers, with anisotropy along the direction of principal extension. We propose a theoretical model, based on the competition between the random disordering field due to the network crosslinks and the external aligning field
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1572-8943
    Keywords: adsorption ; heat capacity ; solid-liquidinterface ; solid monolayer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Heat capacity measurements have been made to investigate the formation of two solid monolayers each of n-pentane, n-heptane and n-dodecane adsorbed on graphite, one at submonolayer coverages and the other coexisting with the liquid. At submonolayer coverages the monolayers are found to melt respectively at 99.8, 151.6, and 217.3 K, well below the bulk melting points. The monolayers coexisting with the liquid melted at 205.6 for heptane and at 287.8 K for dodecane, whereas no evidence was obtained for pentane on the formation of such solid monolayer above the bulk melting point. The order persisting in the liquid near the interface depends upon the length of the molecules. The dodecane monolayers showed another transitions below the melting points both at submonolayer and multilayer coverages.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1435-1536
    Keywords: Colloids ; oscillatory ; shear ; light scattering
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Small-angle light scattering has been used to investigate the structure of sterically stabilised colloidal particles under oscillatory shear. Strain amplitude is significant and evidence for ‘string’ phases under certain conditions is presented.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1435-1536
    Keywords: Key words Disc-shaped particles ; colloidal dispersion ; flocs ; tungsten oxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  To understand the properties of colloidal dispersions it is often vital to prepare model materials with particles that have a small polydispersity in order to make experimental tests of theories or models. However few preparations have been developed that yield anisotropic particles with a narrow size distribution and precise shape. In this paper the preparation of a dispersion of charge stabilized, tungstic acid particles is described. The particles are rectangular and have a length of 7.0 μm, a width of 2.8 μm and a thickness of 0.2 μm. The polydispersity is 8% in length and 10% in width, making it one of the most monodisperse dispersions of anisotropic particles. Each particle is monocrystalline and the crystal orientation is fixed with respect to the particle morphology. Some interesting behavior is observed: these particles aggregate perpendicular and parallel to each other to give flat, ordered flocs when the stability is reduced.
    Type of Medium: Electronic Resource
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  • 9
    Publication Date: 2018-05-30
    Description: High-throughput sequencing methods that multiplex a large number of individuals have provided a cost-effective approach for discovering genome-wide genetic variation in large populations. These sequencing methods are increasingly being utilized in population genetic studies across a diverse range of species. Two side-effects of these methods, however, are (1) sequencing errors and (2) heterozygous genotypes called as homozygous due to only one allele at a particular locus being sequenced, which occurs when the sequencing depth is insufficient. Both of these errors have a profound effect on the estimation of linkage disequilibrium (LD) and, if not taken into account, lead to inaccurate estimates. We developed a new likelihood method, GUS-LD, to estimate pairwise linkage disequilibrium using low coverage sequencing data that accounts for undercalled heterozygous genotypes and sequencing errors. Our findings show that accurate estimates were obtained using GUS-LD, whereas underestimation of LD results if no adjustment is made for the errors.
    Print ISSN: 0016-6731
    Topics: Biology
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