Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Publication Date: 2018-07-31
    Description: Time-resolved collapse and revival of the Kondo state near a quantum phase transition Time-resolved collapse and revival of the Kondo state near a quantum phase transition, Published online: 30 July 2018; doi:10.1038/s41567-018-0228-3 Using terahertz pulses, the quasiparticle dynamics of the heavy-fermion compound CeCu6−xAu are investigated in the vicinity of its quantum critical point.
    Print ISSN: 1745-2473
    Electronic ISSN: 1745-2481
    Topics: Physics
    Published by Springer Nature
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Publication Date: 2015-09-08
    Description: Macromolecular complexes are essential to conserved biological processes, but their prevalence across animals is unclear. By combining extensive biochemical fractionation with quantitative mass spectrometry, here we directly examined the composition of soluble multiprotein complexes among diverse metazoan models. Using an integrative approach, we generated a draft conservation map consisting of more than one million putative high-confidence co-complex interactions for species with fully sequenced genomes that encompasses functional modules present broadly across all extant animals. Clustering reveals a spectrum of conservation, ranging from ancient eukaryotic assemblies that have probably served cellular housekeeping roles for at least one billion years, ancestral complexes that have accrued contemporary components, and rarer metazoan innovations linked to multicellularity. We validated these projections by independent co-fractionation experiments in evolutionarily distant species, affinity purification and functional analyses. The comprehensiveness, centrality and modularity of these reconstructed interactomes reflect their fundamental mechanistic importance and adaptive value to animal cell systems.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wan, Cuihong -- Borgeson, Blake -- Phanse, Sadhna -- Tu, Fan -- Drew, Kevin -- Clark, Greg -- Xiong, Xuejian -- Kagan, Olga -- Kwan, Julian -- Bezginov, Alexandr -- Chessman, Kyle -- Pal, Swati -- Cromar, Graham -- Papoulas, Ophelia -- Ni, Zuyao -- Boutz, Daniel R -- Stoilova, Snejana -- Havugimana, Pierre C -- Guo, Xinghua -- Malty, Ramy H -- Sarov, Mihail -- Greenblatt, Jack -- Babu, Mohan -- Derry, W Brent -- Tillier, Elisabeth R -- Wallingford, John B -- Parkinson, John -- Marcotte, Edward M -- Emili, Andrew -- F32 GM112495/GM/NIGMS NIH HHS/ -- F32GM112495/GM/NIGMS NIH HHS/ -- Canadian Institutes of Health Research/Canada -- England -- Nature. 2015 Sep 17;525(7569):339-44. doi: 10.1038/nature14877. Epub 2015 Sep 7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada. ; Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA. ; Department of Medical Biophysics, Toronto, Ontario M5G 1L7, Canada. ; Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada. ; Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada. ; Department of Biochemistry, University of Regina, Regina, Saskatchewan S4S 0A2, Canada. ; Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany. ; Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas 78712, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26344197" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Datasets as Topic ; *Evolution, Molecular ; Humans ; Multiprotein Complexes/*chemistry/*metabolism ; Protein Interaction Mapping ; *Protein Interaction Maps ; Reproducibility of Results ; Systems Biology ; Tandem Mass Spectrometry
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Publication Date: 2011-04-16
    Description: We present a strategy to design and construct self-assembling DNA nanostructures that define intricate curved surfaces in three-dimensional (3D) space using the DNA origami folding technique. Double-helical DNA is bent to follow the rounded contours of the target object, and potential strand crossovers are subsequently identified. Concentric rings of DNA are used to generate in-plane curvature, constrained to 2D by rationally designed geometries and crossover networks. Out-of-plane curvature is introduced by adjusting the particular position and pattern of crossovers between adjacent DNA double helices, whose conformation often deviates from the natural, B-form twist density. A series of DNA nanostructures with high curvature--such as 2D arrangements of concentric rings and 3D spherical shells, ellipsoidal shells, and a nanoflask--were assembled.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Han, Dongran -- Pal, Suchetan -- Nangreave, Jeanette -- Deng, Zhengtao -- Liu, Yan -- Yan, Hao -- New York, N.Y. -- Science. 2011 Apr 15;332(6027):342-6. doi: 10.1126/science.1202998.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA. dongran.han@asu.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21493857" target="_blank"〉PubMed〈/a〉
    Keywords: DNA/*chemistry ; Models, Molecular ; *Nanostructures ; Nanotechnology ; *Nucleic Acid Conformation
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Publication Date: 2012-06-09
    Description: Phytoplankton blooms over Arctic Ocean continental shelves are thought to be restricted to waters free of sea ice. Here, we document a massive phytoplankton bloom beneath fully consolidated pack ice far from the ice edge in the Chukchi Sea, where light transmission has increased in recent decades because of thinning ice cover and proliferation of melt ponds. The bloom was characterized by high diatom biomass and rates of growth and primary production. Evidence suggests that under-ice phytoplankton blooms may be more widespread over nutrient-rich Arctic continental shelves and that satellite-based estimates of annual primary production in these waters may be underestimated by up to 10-fold.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Arrigo, Kevin R -- Perovich, Donald K -- Pickart, Robert S -- Brown, Zachary W -- van Dijken, Gert L -- Lowry, Kate E -- Mills, Matthew M -- Palmer, Molly A -- Balch, William M -- Bahr, Frank -- Bates, Nicholas R -- Benitez-Nelson, Claudia -- Bowler, Bruce -- Brownlee, Emily -- Ehn, Jens K -- Frey, Karen E -- Garley, Rebecca -- Laney, Samuel R -- Lubelczyk, Laura -- Mathis, Jeremy -- Matsuoka, Atsushi -- Mitchell, B Greg -- Moore, G W K -- Ortega-Retuerta, Eva -- Pal, Sharmila -- Polashenski, Chris M -- Reynolds, Rick A -- Schieber, Brian -- Sosik, Heidi M -- Stephens, Michael -- Swift, James H -- New York, N.Y. -- Science. 2012 Jun 15;336(6087):1408. doi: 10.1126/science.1215065. Epub 2012 Jun 7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Environmental Earth System Science, Stanford University, Stanford, CA 94305, USA. arrigo@stanford.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22678359" target="_blank"〉PubMed〈/a〉
    Keywords: Arctic Regions ; Biomass ; Diatoms/growth & development ; *Eutrophication ; *Ice Cover ; Light ; Nitrates/analysis ; Oceans and Seas ; Photosynthesis ; Photosystem II Protein Complex/analysis ; Phytoplankton/*growth & development ; Seawater/chemistry
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    Publication Date: 2013-03-23
    Description: Engineering wireframe architectures and scaffolds of increasing complexity is one of the important challenges in nanotechnology. We present a design strategy to create gridiron-like DNA structures. A series of four-arm junctions are used as vertices within a network of double-helical DNA fragments. Deliberate distortion of the junctions from their most relaxed conformations ensures that a scaffold strand can traverse through individual vertices in multiple directions. DNA gridirons were assembled, ranging from two-dimensional arrays with reconfigurability to multilayer and three-dimensional structures and curved objects.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Han, Dongran -- Pal, Suchetan -- Yang, Yang -- Jiang, Shuoxing -- Nangreave, Jeanette -- Liu, Yan -- Yan, Hao -- New York, N.Y. -- Science. 2013 Mar 22;339(6126):1412-5. doi: 10.1126/science.1232252.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA. dongran.han@asu.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23520107" target="_blank"〉PubMed〈/a〉
    Keywords: DNA/*chemistry/*ultrastructure ; Models, Molecular ; *Nanostructures ; Nanotechnology/methods ; *Nucleic Acid Conformation
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    Publication Date: 2018-12-21
    Description: Mitochondrial dysfunction is a key determinant of the rare disease lymphangioleiomyomatosis and provides a novel therapeutic target Mitochondrial dysfunction is a key determinant of the rare disease lymphangioleiomyomatosis and provides a novel therapeutic target, Published online: 20 December 2018; doi:10.1038/s41388-018-0625-1 Mitochondrial dysfunction is a key determinant of the rare disease lymphangioleiomyomatosis and provides a novel therapeutic target
    Print ISSN: 0950-9232
    Topics: Medicine
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The soluble antigens ofEntamoeba histolytica trophozoites were analysed in detail by biochemical and immunochemical methods. The antigen was highly complex and heterogeneous as revcaled by Sephacryl S-300 column chromatography, which showed four distinct fractions. The molecular mass of fractions FI, FII, FIII and FIV was 660, 170, 65 and 13 kDa, respectively. Protein was the major constituent in crude soluble antigen (CSA) and fractions FI and FII (67%, 80% and 90%, respectively). Polysaccharide was predominant in the FIII fraction (59%). Antigenic activity observed after different physico-chemical treatments revealed that CSA and FI antigens were predominantly glycoprotein in nature. However, the antigenicity of FIII antigen was greatly reduced after sodium meta-periodate treatment, whereas no alteration in reactivity was discerned after trypsin treatment. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis demonstrated nearly 28 Coomassie blue bands for CSA and 20, 16, 15 and 3 polypeptide bands for the FI, FII, FIII and FIV fractions, respectively. The molecular mass of the polypeptides of these bands ranged from 210 to 20 kDa. Antigenic activity was observed in CSA and in the first three fractions, both in counter immunoelectrophoresis (CIEP) and in enzyme-linked immunosorbent assay (ELISA). However, the highest antigenic activity was noted in fraction FI. Major immunoreactive polypeptides of CSA and FI antigens against whole trophozoite antibody were observed in the 10- to 170-kDa regions. However, major differences in the immunoreactivity of the two antigens were noted at 116 and 14 kDa for FI antigen and at 84, 30 and 20 kDa for CSA. The binding of the FI antibody to the surface of the live parasite and the loss of immunoreactive polypeptides from the FI antibody after its adsorption with live trophozoites ofE. histolytica suggest a correspondence between FI and surface antigens ofE. histolytica. The efficacy of active immunization with CSA and its different fractions showed that antigenicity and immunogenicity were closely associated with the high-molecular-weight proteins, as 91% protection was observed for the FI antigens, whereas the FII and FIII fractions and CSA provided only 41%, 33% and 41% protection, respectively. These data suggest that FI antigen can be used in the serodiagnosis of and immunoprophylaxis against amoebiasis.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    Keywords: CELLS ; CELL ; DIAGNOSIS ; FOLLOW-UP ; TOOL ; DISEASE ; DISTINCT ; cell line ; MOLECULES ; LINES ; PATIENT ; prognosis ; ANTIGEN ; ANTIGENS ; CELL-LINES ; treatment ; MOLECULE ; RECOGNITION ; ACID ; antibodies ; antibody ; IDENTIFICATION ; LYMPHOMA ; CELL-LINE ; leukemia ; LINE ; PURIFICATION ; US ; ACETYLATED SIALIC ACIDS ; ACHATINA-FULICA ; Achatinin-H ; ACID-BINDING LECTIN ; INDIVIDUALS ; SIALIC-ACID ; CHILDREN ; AFFINITY ; MINIMAL RESIDUAL DISEASE ; ACUTE MYELOGENOUS LEUKEMIA ; LEUKEMIC-CELLS ; CHAIN ; CHILDHOOD ; chronic lymphocytic leukemia ; 9-O-acetylated sialoglycoconjugates ; acute lymphoblastic leukemia ; anti-Neu5,9Ac(2) antibody ; CLINICAL STATUS ; INDIAN VISCERAL LEISHMANIASIS ; Neu5,9Ac(2)-binding lectin ; O-ACETYLATION ; SIALOGLYCANS
    Abstract: Sialic acids as terminal residues of oligosaccharide chains play crucial roles in several cellular recognition events. Exploiting the selective affinity of Achatinin-H toward N-acetyl-9-O-acetytneuraminic acid-alpha2-6-GalNAc, we have demonstrated the presence of 9-O-acetylated sialoglycoproteins (Neu5,9Ac(2)-GPs) on lymphoblasts of 70 children with acute lymphoblastic leukemia (ALL) and on leukemic cell lines by fluorimetric HPLC and flow cytometric analysis. This study aims to assess the structural aspect of the glycotope of Neu5,9Ac(2)-GPS(ALL) and to evaluate whether these disease-specific molecules can be used to monitor the clinical outcome of ALL. The Neu5,9Ac(2)-GPs(ALL) were affinity-purified, and three distinct leukemia-specific molecular determinants (135, 120, and 90 kDa) were demonstrated by SDS-PAGE, western blotting, and isoelectric focusing. The carbohydrate epitope of Neu5,9Ac(2)-GPs(ALL) was confirmed by using synthetic sialic acid analogs. The enhanced presence of anti-Neu5,9Ac(2)-GP(ALL) antibody in ALL patients prompted us to develop an antigen-ELISA using purified Neu5,9Ac(2)-GPs(ALL) as coating antigens. Purified antigen was able to detect leukemia-specific antibodies at presentation of disease, which gradually decreased with treatment. Longitudinal monitoring of 18 patients revealed that in the early phase of the treatment patients with lower anti-Neu5,9Ac(2)-GPs showed a better prognosis. Minimal cross-reactivity was observed in other hematological disorders (n = 50) like chronic myeloid leukemia, acute myelogenous leukemia, chronic lymphocytic leukemia, and non-Hodgkin's lymphoma as well as normal healthy individuals (n=21). This study demonstrated the potential of purified Neu5,9Ac(2)-GPs(ALL) as an alternate tool for detection of anti-Neu5,9Ac(2)-GP antibodies to be helpful for diagnosis and monitoring of childhood ALL patients
    Type of Publication: Journal article published
    PubMed ID: 15190007
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    Keywords: CELLS ; GENE ; COMPONENTS ; SERA ; BINDING ; culture ; RECOGNITION ; antibodies ; IDENTIFICATION ; ACUTE LYMPHOBLASTIC-LEUKEMIA ; SURFACE ; ACETYLATED SIALIC ACIDS ; ACHATINA-FULICA ; Achatinin-H ; ACID-BINDING LECTIN ; INFLUENZA-C VIRUS ; Leishmania donovani ; LINKED OLIGOSACCHARIDES ; O-acetylated sialic acid ; sialic acid ; TRANS-SIALIDASE ; TRYPANOSOMA-CRUZI ; UDP-GIcNAc2-epimerase ; VISCERAL LEISHMANIASIS
    Abstract: Sialic acids as terminal residues of oligosaccharide chains play a crucial role in several cellular recognition events. The presence of sialic acid on promastigotes of Leishmania donovani, the causative organism of Indian visceral leishmaniasis, was demonstrated by fluorimetric high- performance liquid chromatography showing Neu5Ac and, to a minor extent, Neu5,9Ac(2). The presence of Neu5Ac was confirmed by GC/MS analysis. Furthermore, binding with sialic acid- binding lectins Sambucus nigra agglutinin (SNA), Maackia amurensis agglutinin (MAA), and Siglecs showed the presence of both alpha2,3- and alpha2,6-linked sialic acids. No endogenous biosynthetic machinery for Neu5Ac could be demonstrated in the parasite. Concomitant western blotting of parasite membranes and culture medium with SNA demonstrated the presence of common sialoglyconjugates (123, 90, and 70 kDa). Similarly, binding of MAA with parasite membrane and culture medium showed three analogous sialoglycans corresponding to 130, 117, and 70 kDa, indicating that alpha2,3- and alpha2,6-linked sialoglycans are adsorbed from the fetal calf serum present in the culture medium. L. donovani promastigotes also reacted with Achatinin- H, a lectin that preferentially identifies 9-O-cetylated sialic acid in alpha2--〉6 GalNAc linkage. This determinant was evidenced on parasite cell surfaces by cell agglutination, ELISA, and flow cytometry, where its binding was abolished by pretreatment of cells with a recombinant 9-O-acetylesterase derived from the HE1 region of the influenza C esterase gene. Additionally, binding of CD60b, a 9-O-acetyl GD3-specific monoclonal antibody, corroborated the presence of terminal 9-O- acetylated disialoglycans. Our results indicate that sialic acids (alpha2--〉6 and alpha2--〉3 linked) and 9-O-acetyl derivatives constitute components of the parasite cell surface
    Type of Publication: Journal article published
    PubMed ID: 12626423
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    Keywords: APOPTOSIS ; CANCER ; CANCER CELLS ; CELLS ; IN-VITRO ; SURVIVAL ; tumor ; TUMOR-CELLS ; BLOOD ; carcinoma ; human ; IN-VIVO ; VITRO ; VIVO ; SITE ; LINES ; MICE ; PATIENT ; COMPLEX ; COMPLEXES ; IFN-GAMMA ; CARCINOGENESIS ; INDUCTION ; T cells ; T-CELLS ; FLOW ; BINDING ; cell cycle ; CELL-LINES ; CYCLE ; treatment ; culture ; ASSAY ; DESIGN ; resistance ; NUMBER ; RATES ; NECROSIS-FACTOR-ALPHA ; CELL-LINE ; LINE ; CANCER-CELLS ; PHENOTYPE ; VISCERAL LEISHMANIASIS ; CANCER-PATIENTS ; INVOLVEMENT ; IMMUNOTHERAPY ; BODY ; CANCER PATIENTS ; PERIPHERAL-BLOOD ; MULTIDRUG-RESISTANCE ; cell lines ; LUNG-CARCINOMA ; INTERFERON-GAMMA ; MONONUCLEAR-CELLS ; INDUCE APOPTOSIS ; FACTOR-ALPHA ; CYTOKINE ; INFILTRATION ; HUMAN CANCER ; INCREASE ; P-GLYCOPROTEIN ; WEIGHT ; INTERVAL ; SIZE ; CANCERS ; in vivo ; peripheral blood ; peripheral blood mononuclear cells ; NECROSIS ; CELLS IN-VIVO ; EHRLICHS ASCITES-CARCINOMA ; HUMAN OVARIAN
    Abstract: Purpose: Previously, we have synthesized and characterized a novel Cu(II) complex, copper N-(2-hydroxyacetophenone) glycinate (CuNG). Herein, we have determined the efficacy of CuNG in overcoming multidrug-resistant cancer using drug-resistant murine and human cancer cell lines. Experimental Design: Action of CuNG following single i.m. administration (5 mg/kg body weight) was tested in vivo on doxorubicin-resistant Ehrlich ascites carcinoma (EAC/Dox)bearing mice and doxorubicin-resistant sarcoma 180-bearing mice. Tumor size, ascitic load, and survival rates were monitored at regular intervals. Apoptosis of cancer cells was determined by cell cycle analysis, confocal microscopy, Annexin V binding, and terminal deoxynucleotidyl transferase - mediated dUTP nick end labeling assay ex vivo. IFN-gamma and tumor necrosis factor-alpha were assayed in the culture supernatants of in vivo and in vitro CuNG-treated splenic mononuclear cells from EAC/Dox-bearing mice and their apoptogenic effect was determined. Source of IFN-gamma and changes in number of T regulatory marker-bearing cells in the tumor site following CuNG treatment were investigated by flow cytometry. Supernatants of in vitro CuNG-treated cultures of peripheral blood mononuclear cells from different drug-insensitive cancer patients were tested for presence of the apoptogenic cytokine and its involvement in induction of apoptosis of doxorubicin-resistant CEM/ADR5000 cells. Results: CuNG treatment could resolve drug-resistant cancers through induction of apoptogenic cytokines, such as IFN-gamma and/or tumor necrosis factor-alpha, from splenic mononuclear cells or patient peripheral blood mononuclear cells and reduce the number of T regulatory marker-bearing cells while increase infiltration of IFN-gamma-producing T cells in the ascetic tumor site. Conclusion: Our results show the potential usefulness of CuNG in immunotherapy of drug-resistant cancers irrespective of multidrug resistance phenotype
    Type of Publication: Journal article published
    PubMed ID: 16857809
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...