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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Chemical Physics 108 (1986), S. 451-459 
    ISSN: 0301-0104
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0921-4534
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0921-4526
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The assembly of the polyhedron envelope in baculovirus-infected cells has been the subject of several studies, yet it is still poorly understood. We have used immunogold-labelled antibodies to two baculovirus proteins, p10 and calyx (also referred to as polyhedron envelope protein or PEP), to follow envelope assembly in AcMNPV-infected tissues ofSpodoptera frugiperda larvae. We show that, in wild type virus, both proteins colocalize in fibrillar structures and associated electron-dense spacers which progress to encircle the polyhedra, as well as in completed polyhedron envelopes. In cells infected with polyhedrin-negative (PH−) viruses, an unusual proliferation of these spacers was observed suggesting a deregulatory event in the envelope assembly process. Results of Northern and Western blot analysis revealed that synthesis of P10 and calyx mRNA and proteins in PH− AcMNPV is unaffected as compared to wild type virus. Taken together, the observed physical and compositional connection between fibrillar structures, spacers and polyhedron envelopes, as well as the abnormal appearance of the spacers in PH− mutants, provide further evidence in support of a cooperative role of these structures in the assembly of the polyhedron envelope.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have immunoprecipitated a phosphoprotein of 35K daltons (35K) common to RSV-transformed chick embryo fibroblasts (CEF) and rodent cells. The phosphorylation of this antigen depends on the expression of thev-src gene and contains phosphotyrosine. The pre-existing 35K protein, of CEF infectedde novo, was further shown to become phosphorylated shortly after the appearance of active pp60 s-src , and about 1 day before morphological transformation. The experiments with RSV-transformed rodent cells have shown that the 35K phosphoprotein is associated with the cellular framework. Another phosphoprotein of 37K was found in the RSV-transformed rodent cells, but not in the transformed CEF. However, this protein was not phosphorylated at tyrosine residues and its phosphorylation persisted, at the restrictive temperature, in cells transformed by ats mutant of RSV.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-5195
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé De Juin 1985 à Mai 1990 nous avons traité 12 patients atteints d'histiocytofibrome malin des parties molles, au niveau des extrémités. Le traitement principal a consisté dans l'association d'une large excision et d'une radiothérapie adjuvante. Une chimiothérapie complémentaire a été administrée aux patients qui pouvaient en tolérer la toxicité. Il y avait 6 hommes et 6 femmes, âgés de 54 ans en moyenne. L'atteinte des membres inférieurs était plus fréquente (8 cas) que celle des membres supérieurs (4 cas). Les types de chirurgie utilisés ont été soit des interventions de conservation des membres, soit des amputations. Tous les malades ont bénéficié de la radiothérapie adjuvante (51.5 Gy en moyenne). Une chimiothérapie par CYVADIC (quatre cycles en moyenne) a été administrée à 6 malades. Il y a eu 2 cas de récidive locale ainsi que 2 métastases pulmonaires. Avec un recul moyen de 41,5 mois (de 12 à 72), le taux de survie à 5 ans de Kaplan-Meier est de 87,5% et le taux de survie à 5 ans sans complications est de 66,7%.
    Notes: Summary Between June 1985 and May 1990 we treated 12 patients with malignant fibrous histiocytoma (MFH) of the limbs by a combination of wide excision and adjuvant radiotherapy. The Enneking surgical staging was II in 11 cases and III in 1. There were 6 women and 6 men, with an average age of 54 years. The leg was involved in 8 patients and the arm in 4. Limb salvage was achieved in 10 patients and amputation was performed in 2. All received adjuvant radiotherapy, with an average of 51.5 Gy. In 6 patients adjuvant CYVADIC chemotherapy was administered for an average of 4 cycles. In the other patients this treatment was not tolerated. Local recurrence occurred in 2 patients and metastases to the lung were seen in a further 2. At an average follow-up of 41.3 months, the Kaplan-Meier estimation of 5 year survival was 87.5%, with a 5 year continuous disease-free survival rate of 66.7%.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    International orthopaedics 17 (1993), S. 293-296 
    ISSN: 1432-5195
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Dix-huit synovialo-sarcomes des extrémités de stade II selon Enneking ont été traités entre mai 1985 et mars 1990 par un traitement combiné de chirurgie et de chimiothérapie associant cyclophosphamide — vincristine — adriamycin — dacarbazine (CYVADIC) en ajoutant ou non une radiothérapie. La chirurgie été une excision large (14 cas) ou une amputation (4 cas). La chimiothérapie par CYVADIC a été administrée en 6 cycles chez tous les patients. La radiothérapie (de 50 à 80 Gy, en moyenne de 62 Gy) a été utilisée chez les 14 patients opérés par excision large et chez 1 patient après amputation. Le suivi moyen des malades est de 34,7 mois avec un minimum d'un an. Le taux de survie estimé à 5 ans (méthode de Kaplan-Meier) est de 78,4% et le taux de patients sans signe de maladie est de 44,4%. La survie sans signe de maladie semble améliorée par une chirurgie ou une chimiothérapie adaptée quand il existe une récidive locale ou des métastases. L'association chirurgie et chimiothérapie par CYVADIC avec ou sans radiothérapie, s'avère une méthode efficace de traitement du synovialo-sarcome des extrémités.
    Notes: Summary From May 1985 to March 1990, 18 cases of Enneking's stage II synovial sarcoma of the extremities were treated by a combination of surgery and CYVADIC chemotherapy with or without radiotherapy. The average follow up was 34.7 months. The estimated 5-year survival rate was 78.4% and the continuously disease-free 5-year survival rate was 44.4%. The results were improved by adequate surgery or chemotherapy when local recurrence or metastases had developed. The combination of surgery and CYVADIC chemotherapy with or without radiotherapy was considered to be an effective method of treating this tumour.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-0509
    Keywords: Key words: Stomach, CT—Stomach, neoplasms—Stomach, radiography—Stomach, US.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report the imaging findings in a case of pedunculated exogastric leiomyoblastoma presenting as a wandering abdominal mass. Ultrasound and computed tomography showed a large, mixed solid and cystic mass in the peritoneal cavity. Computed tomography clearly showed that the mass was connected to the stomach by a narrow stalk. Small bowel follow-through showed subtle extrinsic indentation of the gastric body. The mass wandered from the right to the left side of the peritoneal cavity during various radiologic examinations.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Poly[(R)-3-hydroxybutyric acid] (PHB) was produced at 37 °C by a recombinant Escherichia coli harboring the Alcaligenes eutrophus biosynthesis phbCAB genes in Luria-Bertani media containing glucose at 10–30 g/l at different pH values and the time-dependent changes in the molecular mass of PHB were studied. PHB polymers accumulated within cells while glucose was present in the medium. The number-average molecular mass of PHB decreased with time during the course of PHB accumulation, and the values for PHB were markedly dependent on the cultivation conditions of the E. coli, ranging from 0.5 MDa to 20 MDa. Under specific conditions (pH 6.0), E. coli produced PHB with an extremely high molecular mass (20 MDa). It has been suggested that a chain-transfer agent is generated in E. coli cells during the accumulation of PHB.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract New secretion vectors containing the Bacillus sp. endoxylanase signal sequence were constructed for the secretory production of recombinant proteins in Escherichia coli. The E. coli alkaline phosphatase structural gene fused to the endoxylanase signal sequence was expressed from the trc promoter in various E. coli strains by induction with IPTG. Among those tested, E. coli HB101 showed the highest efficiency of secretion (up to 25.3% of total proteins). When cells were induced with 1 mM IPTG, most of the secreted alkaline phosphatase formed inclusion bodies in the periplasm. However, alkaline phosphatase could be produced as a soluble form without reduction of expression level by inducing with less (0.01 mM) IPTG, and greater than 90% of alkaline phosphatase could be recovered from the periplasm by the simple osmotic shock method. Fed-batch cultures were carried out to examine the possibility of secretory protein production at high cell density. Up to 5.2 g/l soluble alkaline phosphatase could be produced in the periplasm by the pH-stat fed-batch cultivation of E. coli HB101 harboring pTrcS1PhoA. These results demonstrate the possibility of efficient secretory production of recombinant proteins in E. coli by high cell density cultivation.
    Type of Medium: Electronic Resource
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