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  • 1
    Keywords: Life sciences ; Plant science ; Botany ; Life sciences ; Plant Sciences ; Springer eBooks
    Abstract: This volume presents protocols for℗ Brachypodium℗ genomics in numerous areas ranging from marker development, trait evolution, functional genomics, metabolomics, transcriptomics, genomics, and tilling. This book also explores techniques to study the widening genetic base of℗ Brachypodium℗ that will help researchers better understand the model plant using NGS technologies. Written in the highly successful℗ Methods in Molecular Biology℗ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive,℗ Brachypodium Genomics: Methods and Protocols℗ is a valuable resource for bench-oriented molecular biologists and computational biologists working towards further evolving this field
    Pages: XI, 312 p. 69 illus., 52 illus. in color. : online resource.
    ISBN: 9781493972784
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  • 2
    Keywords: Life sciences ; Plant breeding ; Plant physiology ; Life sciences ; Plant Breeding/Biotechnology ; Plant Genetics & Genomics ; Plant physiology ; Springer eBooks
    Description / Table of Contents: Expression analysis and genome annotations with RNA sequencing -- The application of Next Generation Sequencing techniques to Plant Epigenomics -- Whole genome sequencing to identify genes and QTL in rice -- Variant calling using NGS data in European aspen (Populus tremula) -- Leafy Spurge Genomics: A Model Perennial Weed To Investigate Development, Stress Responses, And Invasiveness -- Utilization of NGS and proteomic-based approaches to gain insights on cellular responses to singlet oxygen and improve energy yields for bacterial stress adaptation -- Experimental evolution and next generation sequencing illuminate the evolutionary trajectories of microbes -- Plant carbohydrate active enzyme (CAZyme) repertoires: a comparative Study -- Metagenomics of Plant- Microbe Interactions -- Genes and trans-factors underlying embryogenic transition in plant soma-cells -- Bioinformatics tools to analyze the proteome and genome data -- High through-put transcriptome analysis of plant stress responses -- CNV and structural variation in plants: prospects of NGS Approaches
    Abstract: This work is a compiled catalogue of such findings, where several NGS technologies ranging from the genomics, transcriptomics, metagenomics, single cell genomics, QTL, patho-genomics and patho-transcriptomics have been applied to delineate the mystery of the associated mutations, biological pathway transitions, transcriptional fluxes and patterns of host associated or adaptations to certain climatic conditions. The aims and scope of this book focuses more on the biological underpinning to initiate the cross-talks across the traits acquired or lost during the course of evolution. The structured framework of the present volume provides the applicative point of view of the NGS technologies and demonstrates the conceptual way of linking the experimentation to the NGS technologies, to aid in researchers to place their biological hypothesis in a larger context
    Pages: XII, 241 p. 40 illus., 31 illus. in color. : online resource.
    ISBN: 9783319171579
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