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  • 1
    ISSN: 1613-9674
    Keywords: Panoramic radiography ; TM disorders ; Osseous changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The usefulness and the limitations of panoramic radiography for the diagnosis of temporomandibular (TM) disorders were evaluated. The condylar shape appearance and the osseous changes were evaluated in asymptomatic 150 subjects (control subjects) and in 142 patients with the TM disorders who were examined by panoramic radiography. The position of the flattening and the osseous changes including osteophyte, bone erosion and osteosclerosis were objectively analyzed to determine the condylar shape. The incidence of the composite flattening type, which exhibits flattening on both the anterior and on other surfaces, was three times higher in the TM patients compared to controls. Thus, this composite type may indicate the presence of TM disorders. The incidence of osseous changes in the patients with TM disorders was significantly higher than controls and increased further in the group with the permolar and/or molar missing. This suggests that the occlusion of molar, teeth is an important factor related to the development of the TM disorders. It was concluded that panoramic radiography was useful for detecting TM disorders, at least in those with osseous changes or flattening.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1613-9674
    Keywords: Computed tomography ; Simusitis ; Paranasal sinuses CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To assess the usefulness of CT images for the diagnosis of suspected odontogenic maxillary sinusitis, 52 patients with maxillary sinusitis who had received CT examination were retrospectively analyzed. maxillary sinus ostium was established on coronal CT image in 20 out of 55 lesions in those 52 patients. However, a considerable number of patients who had inflammatory disease not only in the maxillary sinus but also in other paranasal sinuses (38/55) or in the nasal meatus (34/55) were included in those 52 patients. Concerning the opening of the ostium, the clinical diagnosis is frequently different from the diagnosis made using CT. The CT findings of the maxillary sinus ostium, nasal meatus and other paranasal sinuses were thus important in planning the treatment for patients with maxillary sinusitis in dentistry, as in otorhinolaryngology.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Accumulated evidence indicates that proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase δ and forms tight association with DNA replication sites during DNA replication or DNA repair synthesis. In this study, such PCNA complex formation was investigated by the indirect immunofluorescence method, using both normal human fibroblasts and those derived from a xeroderma pigmentosum group A (XP-A) patient. XP-A fibroblasts in both proliferating and quiescent states did not show any differences from normal fibroblasts in the properties of PCNA-staining in the untreated conditions. The PCNA complex formation was induced in quiescent normal fibroblasts by both ultraviolet light (UV)- and X-irradiation, whereas in XP-A fibroblasts it was induced by X-irradiation, but not by UV-irradiation. However, PCNA complex was induced in quiescent XP-A fibroblasts by UV-irradiation when the cells had previously incorporated 5-bromodeoxyuridine (BrdU). These observations indicate a close correlation of PCNA complex formation and unscheduled DNA synthesis (UDS). Thus, it was concluded that PCNA complex formation was commonly induced in at least three conditions to produce UDS in spite of different types of DNA damages and DNA repair mechanisms.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We examined the role of the factor deficient in xeroderma pigmentosum group A (XP-A) cells in the formation of proliferating cell nuclear antigen (PCNA) complex with DNA in the DNA repair process in human fibroblasts following cisdiamminedichloroplatinum (CDDP)-treatment. Immunofluorescence staining after methanol fixation was used to detect the PCNA complex formation. When quiescent normal cells were PCNA-stained at 3 h after 100 μM CDDP treatment for 1 h, almost all nuclei of the cells showed a punctuated staining pattern. On the other hand, nuclei of XP-A cells were not stained. These results were the same with the findings following 10J/m2 of ultraviolet light (UV)-irradiation. The quantitative analysis of the PCNA immunofluorescence intensity of normal cells revealed that the mean intensity was increased by 4.8 times by the CDDP-treatment and 6.1 times by the UV-irradiation, compared with that of untreated cells. The intensities among nuclei ranged widely in both treatments. In contrast, the mean intensity was not increased in XP-A cells by the same treatments. However, when XP-A cells were fused with normal cells with polyethylene glycol (PEG) treatment, the nuclei of the XP-A cells showed positive PCNA-staining following CDDP-treatment or UV-irradiation in almost all cases. These results suggest that the PCNA complex formation may play a role in the DNA repair process after the step where the factor deficient in XP-A cells is involved following CDDP-treatment as well as following UV-irradiation. © 1992 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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