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  • 1
    Call number: YY Diss Schu/Mag
    Keywords: DKFZ-publications / academic dissertations
    Notes: Thesis (Ph.D) -- Ruprecht-Karls-Universität, Heidelberg, 1981.
    Pages: 99 p.
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    YY Diss Schu/Mag departmental collection or stack – please contact the library
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  • 2
    Call number: WN620:68
    Keywords: Electromagnetic Fields / adverse effects ; Telephone ; Risk Assessment
    Pages: numerous pagings.
    ISBN: 3-89336-399-8
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    WN620:68 available
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  • 3
    Call number: 01-SichIng:246 ; 01-SichIng:247 ; 01-SichIng:260
    Notes: Not kept up to date.
    Pages: loose-leaf
    ISBN: 3-503-02085-3
    Signatur Availability
    01-SichIng:246 departmental collection or stack – please contact the library
    01-SichIng:247 departmental collection or stack – please contact the library
    01-SichIng:260 departmental collection or stack – please contact the library
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  • 4
    ISSN: 1432-0738
    Keywords: Bromination ; Thin Layer Chromatography ; Barbiturates (Allional, etc. see German Key words) ; Bromierung ; Dünnschichtchromatographie ; TRT-Technik ; Barbiturate (Allional, Amobarbital, Brallobarbital, Butabarbital, Butallylonal, Butethal, Evipan, Itobarbital, Luminal, Medomin, Metharbital, Nealbarbital, Pentobarbital, Phanodorm, Prominal, Propallylonal, Rectidon, Secobarbital, Speda, Talbutal, Veronal)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Durch Behandeln mit Brom am Startpunkt zweidimensionaler Dünnschichtchromatogramme lassen sich viele Barbiturate reproduzierbar in Derivate überführen. Die nachfolgende Trennung gibt Aufschluß über die Struktur und führt anhand der Rf-Werte der Bromaddukte (die sich nach einem mitgeteilten Schema bilden) in den meisten Fällen zu einer genauen Spezifizierung. Die Methode ist auch auf Barbituratgemische anwendbar.
    Notes: Abstract By means of treatment with bromine at the starting point of two dimensional thin layer chromatograms, many barbiturates may be converted to reproducible derivatives. The subsequent separation provides structural information and in nearly all investigated examples permits exact specification of the brominated compounds by way of their Rf values, a scheme of which is included in the article. The method is also applicable to mixtures of barbiturates.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0738
    Keywords: Benzodiazepines ; Lorazepam ; Thin Layer Chromatography ; Benzophenone Derivatives ; Bratton-Marshall reagent ; Benzodiazepine ; Lorazepam ; Ddnnschichtchromatographie ; Benzophenonderivate ; Bratton-Marshall-Reagens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird eine Methode zum Schnellnachweis von Lorazepam mit Hilfe dünnschichtchromatographischer Untersuchung des bei der sauren Hydrolyse entstehenden 2-Amino-2′,5-dichlor-benzophenons beschrieben.
    Notes: Abstract A method is described for the detection of Lorazepam by means of thin layer-chromatographic examination of 2-Amino-2′,5-dichlor-benzophenone obtained by acid hydrolysis.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0738
    Keywords: Bromureides ; Carbromal ; Metabolites ; Detection in Urine ; UV Spectra ; Bromoureide ; Carbromal ; Metabolite ; Nachweis im Urin ; UV-Spektren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird eine Methode zum Nachweis von Bromoureidmetaboliten im Urin nach der Einnahme therapeutischer und suicidaler Dosen mit Hilfe UV-photometrischer Untersuchungen mitgeteilt. Durch Unterteilung der Inhaltsstoffe des aus dem angesäuerten Urin gewonnenen ätherischen Extraktes mit wäßrigen Pufferlösungen steigenden pH-Wertes (7,10,5,14) werden im letzten Schritt mit 2 n-Natronlauge Bromoureide und einige Metabolite in die wäßrige Phase überführt. Die alkalische Zersetzung dieser Substanzen wird durch eine fortlaufende wiederholte Registrierung der UV-Spektren verfolgt. Zusammenhänge mit dem Zeitpunkt der Einnahme werden mitgeteilt und diskutiert.
    Notes: Abstract A UV photometric method for the detection of bromureid metabolites in urine after ingestion of therapeutic and suicidal doses is described. An etherial extract is gained from the acidified urine. The substances contained in it are separated with aqueous buffers of increasing pH (7,10,5,14). The last step is performed with 2 N sodium hydroxide solution and transfers bromureides and some metabolites to the aqueous phase. The alkaline decomposition of these substances is monitored by continuous observation of the UV spectra. Connections with the time of ingestion are reported and discussed.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-904X
    Keywords: CYP3A4 ; Pgp ; Caco-2 ; LLC-PK1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. To develop model polarized cell systems expressingcytochrome P4503A4, NADPH P450 reductase, and P-glycoprotein (Pgp). Methods. LLC-PK1 and derivative L-MDR1 cells stably expressing Pgp,the product of the multidrug resistance gene (MDR1), were transfectedstably using either a mammalian neomycin selectable expression vector(CYP3A4-Neo) or an episomal vector based on Epstein—Barr virus(CYP3A4-Hygro). These CYP3A4 expressing cells were compared withLLC-PK1, L-MDR1, or Caco-2 cells transduced with Adenovirus-3A4vector (Ad3A4) with or without simultaneous Adenovirus-P450 Reductase(AdRed) transduction. Cells were characterized for expression of CYP3A4protein and CYP3A4 mediated metabolism towards midazolam and testosterone. Analysis of membrane integrity and drug transport assays wereperformed to determine whether infection with recombinant Ad3A4 ±AdRed affected Pgp function. Results. The rank order of optimal CYP3A4 expression and activitiesin LLC-PK1 and L-MDR1 cells from highest to lowest was cellsco-transduced with Ad3A4 plus AdRed 〉〉 Ad3A4 〉〉〉CYP3A4-Hygro 〉 CYP3A4-Neo. Similarly, coexpression of Ad3A4 plus AdRedled to enhanced CYP3A4 mediated metabolism in Caco-2 cells overcells with Ad3A4 alone. Incubation of transwell cultured cells expressing Ad3A4/AdRed with midazolam led to readily detectable metabolitein the medium. In microsomes from Caco-2 and LLC-PK1 cells, eachco-transduced with Ad3A4/AdRed, Vmax values for testosterone6β-hydroxylase activity ranged from 414 to 1350 pmoles/min/mg,respectively. For either Caco-2 or LLC-MDR1 cells, TEER values and therate of apical to basal and basal to apical transport of vinblastine ordigoxin were similar in cells with and without Ad3A4/Red transduction. Conclusions. Polarized cellular systems coexpressing Ad3A4, AdRed,and the MDR1/Pgp transporter were developed and characterized. Theresults document the utility of these polarized model systems forsimultaneous drug transport/drug metabolism studies. Since the experimentalapproach can be adapted to study the interplay of multipleenzyme/transporting systems, it may find significant application as a screeningtool for the pharmaceutical industry and as a more basic research toolto study the kinetics of intestinal drug bioavailability.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1618-2650
    Keywords: Nachw. von 5-Phenyl-1,4-benzodiazepinen, Chlorazepate, Chlordiazepoxid, Diazepam, Medazepam, Nitrazepam ; Chromatographie, Dünnschicht ; zweidimensional, saure Hydrolyse Nachw. von Tranquilizern ; Chromatographie ; Dünnschicht ; 5-Phenyl-1,4-benzodiazepine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es wird eine Analysenmethode zum Nachweis und zur Trennung von fünf 5-Phenyl-1,4-benzo-diazepinen (Chlorazepate, Chlordiazepoxid, Diazepam, Medazepam, Nitrazepam) beschrieben. Nach einer Trennung in der ersten Laufrichtung eines zweidimensionalen Dünnschicht-Chromatogrammes werden die Benzodiazepine durch Behandeln mit Salzsäure in Benzophenonderivate überführt. Die nachfolgende Trennung in der zweiten Laufrichtung gibt Strukturhinweise und führt zu einer empfindlichen Charakterisierung. Die Nachweisgrenze beträgt etwa 0,02 μg.
    Notes: Abstract An analysis method for the separation and detection of five 5-phenyl-1,4-benzodiazepines (Chlorazepate, Chlordiazepoxid, Diazepam, Medazepam, Nitrazepam) is described. After separation in the first dimension of two-dimensional thin-layer chromatograms, the benzodiazepines may be converted to benzophenone derivatives by means of treatment with hydrochloric acid. The subsequent separation in the second dimension provides structural information and permits exact specification by way of Rf-values and Bratton-Marshall detection. With this method contents as low as 0.02 μg can be detected.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-2072
    Keywords: Pseudoconditioning ; Classical conditioning ; Avoidance conditioning ; Brain noradrenaline ; Brain dopamine ; Caudate ; Accumbens ; Hypothalamus ; Amygdala
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Rats were trained to perform shuttle responses to a buzzer in four different situations: Pseudoconditioning (buzzers and footshocks presented at random), classical conditioning (buzzers and footulus paired on every trial), avoidance without stimulus pairing (buzzer-shock intervals varied at random, shocks contingent upon the nonemission of a shuttle response to the preceding buzzer), and standard two-way avoidance (buzzers paired to shocks, but the latter omitted every time there was a shuttle to the buzzer). Animals were killed immediately after the last trials and the noradrenaline and dopamine content of their hypothalamus, amygdala, caudate nucleus, and nucleus accumbens was determined. There were falls of dopamine content in the caudate and accumbens and falls of noradrenaline levels in all structures except the caudate after the pseudoconditioning test. Noradrenaline levels were normal, and dopamine levels were partially recovered, in the animals submitted to the other training situations. Thus learning factors (stimulus pairing and/or the avoidance contingency) offset the depleting influence of footshocks per se on both catecholamines in at least the structures studied.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Recent studies have suggested that a labile transcriptional repressor protein is important in the regulation of pgp mRNA expression. However, cycloheximide (CHX) the protein synthesis inhibitor used, can increase mRNAs by either stabilizing the mRNA transcript or directly activating gene transcription. To determine whether CHX posttranscriptionally increased pgp mRNA, we compared the effect of CHX, which inhibits protein synthesis by stabilizing polysomes, with puromycin (PURO), which inhibits protein synthesis by polysome destabilization. In rat hepatocytes, CHX induced pgp2 mRNA, and the increase was proportional to the degree of protein synthesis inhibition. In contrast, despite almost complete inhibition of protein synthesis, PURO did not induce pgp2 mRNA. Further studies demonstrated that PURO pretreatment could block pgp2 mRNA induction by CHX. Likewise, in cultures of primary human hepatocytes CHX, but not PURO, induced MDR1 mRNA. A polymerase chain reaction assay was developed to assess whether CHX treatment altered the length of the 3′-untranslated region (UTR) of pgp2. CHX treatment time dependently increased the length of the pgp2 3′-UTR. To determine whether CHX acts as a transcriptional agonist, we performed nuclear run-off analysis and found no increase in pgp2 gene transcription compared to untreated control. Further, transcription studies were performed by transiently transfecting HepG2 cells with plasmids containing 5′ segments of human MDR1 fused with the reporter chloramphenicol acetyltransferase (CAT). These plasmids were not transcriptionally activated by CHX. In summary, our results cast doubt on the existence of a labile transcriptional repressor protein for pgp. Furthermore, these are the first studies to demonstrate that polysomal destabilization by PURO can block CHX induction of pgp. © 1995 Wiley-Liss, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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