Blackwell Publishing Journal Backfiles 1879-2005
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Process Engineering, Biotechnology, Nutrition Technology
A computerized laser diffractometer was constructed to measure lengths of sarcomeres in muscle tissue. Calibration and fault-finding procedures were incorporated to ensure reliable performance. The computer provided outputs of sarcomere length, first-order width and printed statistics from a series of measurements. Sarcomere lengths obtained by the diffractometer method were compared with microscopically determined sarcomere lengths on freeze-sectioned raw or fixed porcine M. Longissimus dorsi muscles. The diffractometer yielded somewhat lower mean values than the microscope but both types of measurements were found to be significantly (P 〈 0.001) correlated with the corresponding Warner-Bratzler shear values. The primary advantage of using the computerized diffractometer as compared to manual measurements of diffraction patterns was the possibility of using freeze-sectioned raw samples.
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