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  • 1
    Call number: YY Diss Schn/Mag
    Keywords: DKFZ-publications / academic dissertations
    Notes: Titel wurde ermittelt.
    Pages: 97 p.
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  • 2
    ISSN: 1432-2048
    Keywords: Cell wall ; Endochitinase ; Solanaceae ; Style ; Transmitting tract
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An abundant, pistil-specific basic protein has been purified and characterized from potato (Solanum tuberosum L.). A polymerase chain reaction (PCR) probe was generated for the corresponding gene using oligonucleotides based on internal peptide sequences of the protein, and the PCR probe was further employed to isolate cDNA and genomic clones. The sequence of the gene exhibits up to 70% similarity to previously described endochitinase class 1a protein sequences, and the purified protein possesses chitinase {poly[1, 4-(N-acetyl-β-D-glucosaminide)] glucanohydrolase, EC 3.2.1.14} activity. The protein, termed SK2, has been located by immunocytochemistry to the intercellular matrix of the stylar transmitting tract. Immunoblot analysis has shown SK2 to be distinct from the wound-induced chitinases of potato. The SK2-class of chitinase is restricted in its distribution within the Solanaceae to the sub-family Solanoidae, which includes cultivated tomato and potato species. It was absent from the Cestroidae species tested (Petunia hybrida, Nicotiana tabacum). A role for SK2 endochitinase in protecting the ovary against pollen-tubemediated pathogen ingress is proposed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2048
    Keywords: Cell wall ; Endochitinase ; Solanaceae ; Style ; Transmitting tract
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An abundant, pistil-specific basic protein has been purified and characterized from potato (Solanum tuberosum L.). A polymerase chain reaction (PCR) probe was generated for the corresponding gene using oligonucleotides based on internal peptide sequences of the protein, and the PCR probe was further employed to isolate cDNA and genomic clones. The sequence of the gene exhibits up to 70% similarity to previously described endochitinase class 1a protein sequences, and the purified protein possesses chitinase {poly[1, 4-(N-acetyl-β-D-glucosaminide)] glucanohydrolase, EC 3.2.1.14} activity. The protein, termed SK2, has been located by immunocytochemistry to the intercellular matrix of the stylar transmitting tract. Immunoblot analysis has shown SK2 to be distinct from the wound-induced chitinases of potato. The SK2-class of chitinase is restricted in its distribution within the Solanaceae to the sub-family Solanoidae, which includes cultivated tomato and potato species. It was absent from the Cestroidae species tested (Petunia hybrida, Nicotiana tabacum). A role for SK2 endochitinase in protecting the ovary against pollen-tubemediated pathogen ingress is proposed.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2048
    Keywords: Abscisic acid (desiccation tolerance) ; Callus (desiccation tolerance) ; Craterostigma ; Desiccation tolerance (protein, RNA induction) ; Resurrection plant RNA induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Leaves of the resurrection plant Craterostigma plantagineum Hochst, can be desiccated up to 1% relative water content and are still viable after rehydration. To clone genes related to this extreme desiccation tolerance, an in-vitro system was first developed which allows the induction of the same resurrection response in callus tissue upon treatment with abscisic acid (ABA). Several proteins and in-vitro-synthesized polypeptides were then identified which can be induced both in desiccation-tolerant, naturally dried leaves and in ABA-treated calli surviving after rehydration. Complementary-DNA clones corresponding to mRNAs expressed only in desiccation-tolerant tissues were obtained and classified into several gene families. In hybrid-selected translation experiments, representative cDNA clones were associated with water stress and ABA-inducible polypeptides abundantly expressed in dried leaves and ABA-treated calli. The expression pattern of several of these abundant transcripts was analyzed in RNA-hybridization experiments. Upon stress or ABA treatment the transcription levels increased rapidly, but they declined after relief from the stress state. This, together with data on genomic copy numbers indicated that a set of abundantly expressed genes are involved in the desiccation process of resurrection plants. Data on endogenous ABA contents before and after stress applications and on the physiological effects of exogenous ABA treatments indicate that in Craterostigma plantagineum the induction of an extreme desiccation tolerance is mediated by this plant hormone.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Chloroplast protein ; Desication-related polypeptide ; Resurrection plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A number of desiccation-related and abscisic-acid (ABA)-inducible transcripts have been isolated from the resurrection plant Craterostigma plantagineum (Scrophulariaceae). They have been analysed at the transcriptional level (D. Bartels et al., 1990, Planta 181, 27–34) and their nucleotide sequences determined (D. Piatkowski et al., 1990, Plant Physiol. 94, 1682–1688). Three such genes encoded polypeptides with substantial homologies to proteins abundantly expressed during late embryogenesis in many higher plants; two other genes encoded novel transcripts. The temporal expression patterns of these gene products and their distribution in different organs of the plant and in callus tissues have now been analysed immunologically. For this, in-situ RNA hybridizations and immunocytochemical studies using tissue sections were carried out at both the light and electron microscope level. All of the products were found to be present in leaf tissue, and some were also found in roots and in seeds. Three desiccation-related proteins were localized in the cytosol, while two others, one associated with the thylakoid membranes, the other soluble in the stroma, were detected in the chloroplast. In C. plantagineum the severe ultrastructural changes observed during the desiccation-rehydration process indicate the need for protectants: the gene products characterized in this publication may be good candidates for this role.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5028
    Keywords: Chloroplast targeting ; desiccation tolerance ; drought-related proteins ; resurrection plant (Craterostigma) ; transgenic tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three cDNAs encoding desiccation-induced proteins from the resurrection plant Craterostigma plantagineum were each ligated to a triplicated CaMV 35S promoter and a nopaline synthase 3′-flanking region in an Agrobacterium vector and introduced into tobacco. Transgenic plants expressed the encoded Craterostigma proteins at high levels. This did not lead to changes in the phenotype, in the growth habit or in basic photosynthetic parameters. In tobacco, one protein was targeted to the chloroplast stroma which is its normal location in Craterostigma. These desiccation-related proteins are not sufficient per se to increase drought tolerance as measured by ion-leakage tests.
    Type of Medium: Electronic Resource
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