Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  Gemeinsame Jahrestagung der Gesellschaft für Medizinische Ausbildung (GMA) und des Arbeitskreises zur Weiterentwicklung der Lehre in der Zahnmedizin (AKWLZ); 20170920-20170923; Münster; DOC107 /20171124/
    Publication Date: 2017-11-24
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  Jahrestagung der Gesellschaft für Medizinische Ausbildung (GMA); 20140925-20140927; Hamburg; DOCP184 /20140911/
    Publication Date: 2014-09-12
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Keywords: proliferation ; SYSTEM ; DISEASE ; PROTEIN ; MICE ; PATIENT ; MARKER ; ANTIGEN ; ANTIGENS ; T cell ; T cells ; T-CELL ; T-CELLS ; culture ; antibodies ; antibody ; MOUSE ; NERVOUS-SYSTEM ; LESIONS ; NUMBER ; COMPONENT ; DAMAGE ; cytoskeleton ; NETHERLANDS ; CD8(+) ; CENTRAL-NERVOUS-SYSTEM ; pathology ; AMYOTROPHIC-LATERAL-SCLEROSIS ; MULTIPLE-SCLEROSIS ; INTERFERON-GAMMA ; inflammation ; CD4(+) T-CELLS ; AUTOIMMUNE ENCEPHALOMYELITIS ; SERUM ; AUTOIMMUNITY ; IMMUNIZATION ; CYTOKINE ; RECOMBINANT ; RE ; SERUM ANTIBODIES ; LEVEL ; multiple sclerosis ; USA ; animal model ; central nervous system ; DEGENERATION ; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS ; correlates ; SENSORY NEURONOPATHY ; axonal damage ; MS LESIONS ; neurofilament light ; PROGRESSIVE MULTIPLE-SCLEROSIS ; spastic paresis
    Abstract: Axonal damage is the major cause of irreversible neurologic disability in patients with multiple sclerosis. Although axonal damage correlates with antibodies against neurotilament light (NF-L) protein, a major component of the axonal cytoskeleton, the possible pathogenic role of autoirnmunity to axonal antigens such as NF-L has so far been ignored. Here we show that Biozzi ABH mice immunized with NF-L protein develop neurologic disease characterized by spastic paresis and paralysis concomitant with axonal degeneration and inflammation primarily in the dorsal column of the spinal cord. The inflammatory central nervous system lesions were dominated by F4/80' macrophages/microglia and relatively low numbers of CD4(+) and CD8(+) T-cells. In splenocyte cultures, proliferation to NF-L was observed in CD4(+) T-cells accompanied by the production of the proinflammatory cytokine interferon-gamma. Elevated levels of circulating antibodies recognizing recombinant mouse NF-L were present in the serum, and immunoglobulin deposits were observed within axons in spinal cord lesions of mice exhibiting clinical disease. These data provide evidence that autoimmunity to NF-L protein induces axonal degeneration and clinical neurologic disease in mice, indicating that autoimmunity to axonal antigens, as described in multiple sclerosis, may be pathogenic rather than acting merely as a surrogate marker for axonal degeneration
    Type of Publication: Journal article published
    PubMed ID: 17413320
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    facet.materialart.
    Unknown
    German Medical Science GMS Publishing House; Düsseldorf
    In:  Gemeinsame Jahrestagung der Gesellschaft für Medizinische Ausbildung (GMA) und des Arbeitskreises zur Weiterentwicklung der Lehre in der Zahnmedizin (AKWLZ); 20150930-20151003; Leipzig; DOCV416 /20150831/
    Publication Date: 2015-09-01
    Keywords: ddc: 610
    Language: German
    Type: conferenceObject
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1432-1106
    Keywords: Presynaptic calcium uptake ; Postsynaptic calcium uptake ; Adenosine ; Theophylline ; Adenosine deaminase ; 4-Aminopyridine ; Rat hippocampus ; Synaptic transmission
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Orthodromically and antidromically evoked field potentials, as well as changes in the extracellular calcium concentration [Ca2+]0 were measured with ion selective/reference electrodes in area CA1 of rat hippocampal slices. Synaptic transmission was blocked by a low calcium, high magnesium medium. After cutting through the alveus, stratum pyramidale (Spyr) and part of stratum radiatum (Srad), repetitive electrical stimulation of Schaffer collaterals and commissural fibers elicited decreases of [Ca2+]0 in Srad, the synaptic area, but not in Spyr, the soma layer of the pyramidal neurons. This indicates the absence of a measurable somatic Ca2+ influx due to postsynaptic activation and therefore, the decrease of [Ca2+]0 in Scrad presumably reflect presynaptic Ca2+ entry. Antagonists of adenosine action such as adenosine deaminase and theophylline had no effects Ca2+ entry whereas 4-AP enhanced calcium signals in Scrad considerably. In some cases small [Ca2+]0 decreases in Spyr appeared after treatment with 4-AP although field potentials did not reveal postsynaptic components. When 4-AP and antagonists of adenosine action were combined, a partial recovery of synaptic transmission was consistently seen during the course of repetitive stimulation. This was indicated by large decreases of [Ca2+]0 in Spyr as well as by the generation of postsynaptic field potentials. The latter appeared late during the train pointing to frequency potentiation and a presynaptic site of action. The findings indicate that physiological levels of adenosine in the order of 1 μM have a powerful modulatory role on synaptic transmission by depressing presynaptic transmitter release. This seems to result not from an influence on presynaptic calcium uptake, but rather from changing the intracellular level of calcium or its coupling to the secretory process.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The influence of pH on the propylation with sodium tetra(n-propyl)borate of butyl- and phenyltins as well as for trimethyl- and triethyllead was investigated. Ethylation and propylation with tetraalkylborates were compared with regard to derivatization yields and figures of merit for organotin compounds in real water samples. Similar results for limit of detection (3–12 ng/L as tin), derivatization yield (40–100%) and relative standard deviation of the method (3–10%) were achieved for derivatization with the two tetraalkylborates. Propylation is thus the preferred method for the simultaneous determination of environmentally relevant organotin and organolead compounds. The handling of the hygroscopic and air sensitive reagents NaBEt4 and NaBPr4 was simplified by dissolving them in tetrahydrofurane. The reagent solutions in tetrahydrofurane can be stored for at least one month at ¶4 °C in the dark without observing any decrease in the derivatization efficiency.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1432-1106
    Keywords: 6-Hydroxydopamine ; Neuronal uptake ; Central nervous system ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Tritiated 6-hydroxydopamine (6-OHDA) was injected into the lateral ventricle of rats and rabbits and visualized by means of autoradiography. In unfixed frozen sections, radioactive material was found penetrating about 2 mm into the periventricular tissue within 4–48 hours after injection. Radioactivity was localized preferably in particular nuclei, leaving the fiber bundles nearly free of label. In formalin-fixed paraplast-embedded sections the appearance of silver grain clusters was the very characteristic finding representing most probably uptake into presynaptic terminals. Labelling pattern suggests highly selective uptake of 6-OHDA by terminals of noradrenaline and dopamine-containing neurones; also a few single neurones of particular mesencephalic nuclei showed uptake by their nerve cell soma. A specific uptake mechanism for 6-OHDA which is most likely identical with the monoamine pump may explain the well-known selectivity of the pharmacological action of 6-OHDA. Mapping of silver grain clusters and an analysis of the frequency by which they occur in different areas of the brain were performed.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    ISSN: 1432-1106
    Keywords: Axonal flow ; Excitation ; Single-cell-injection ; Autoradiography ; Cat Motoneurons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Tritiated glycine has been injected intracellularly into cat motoneurons by means of cross barrel iontophoresis through microelectrodes. The amino acid is incorporated into proteins in the cell body and the synthesized radioactive proteins have been localized by autoradiography. The single-cell-injection technique has several advantages over the usual intraperitoneal, intravenous or intrathecal injection, e.g. distribution of the radiochemical is strictly confined to the injected neuron; the amount of injected substance can be estimated; it is easy to investigate simultaneously electrophysiological and morphological characteristics of a defined neuron. Since the injected cell body releases radioactive proteins into the axon, the transport of these substances can be studied under various conditions. In this study the influence of antidromic stimulation has been studied. Stimulated motoneurons demonstrate higher radioactivity in their cell bodies than unstimulated ones, reflecting an increased protein synthesis. The differences are even more pronounced in the axons of stimulated cells when compared with unstimulated ones. A significant, higher amount of radioactive material — up to 100% increase as demonstrated by silver grain counting — can be seen in the axons of stimulated neurons. Although considerable differences seem to exist for the quantity of the exported proteins no significant differences have been detected for the velocity of transportation. In axons of stimulated and unstimulated neurons proteins advance toward the periphery at the same rate.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The outer membrane protein, PagC, of Salmonella typhimurium was converted into a secreted protein by linking the 61-amino-acid long, C-terminal signal sequence of the E. coli hemolysin protein (HlyAS) to the mature PagC peptide. This PagC-HlyAS fusion protein was expressed and efficiently secreted into the culture supernatant by E. coli upon complementation with the hemolysin secretion proteins HlyB and HlyD. Polyclonal antibodies raised against this fusion protein not only recognized PagC in the membrane fraction of all salmonellae by Western blotting, but also reacted with proteins of smaller size in other gram-negative bacteria tested. A monoclonal antibody against the PagC-HlyAS fusion protein recognized only PagC in membrane fractions. The antibody-binding domain was determined using synthetic peptides derived from specific PagC domains. Sera from Salmonella-infected human patients and from a rabbit infected with S. typhimurium did not react with PagC in immunoblots, suggesting that PagC may not be recognized as a major antigen by the humoral immune system.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    ISSN: 0020-711X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...