Description / Table of Contents:
The neural crest: a remarkable model system for studying development and disease -- Addressing interdisciplinary difficulties in developmental biology / mathematical collaborations: a neural crest example -- Generation of neural crest progenitors from human pluripotent stem cells -- Fully defined and xeno-free induction of hPSCs into neural crest using top-down inhibition of BMP signaling -- Deciphering the neural crest contribution to cephalic development with avian embryos -- In ovo electroporation of plasmid DNA and morpholinos into specific tissues during early embryogenesis -- Ex vivo culture and manipulation of mouse neural crest cells from primary embryonic tissue explants -- Technologies for live imaging of enteric neural crest derived cells -- Mouse embryo culture for the study of neural crest cells -- Laser capture microdissection of murine embryonic neural crest cells -- In vivo and in vitro quantitative analysis of neural crest cell migration -- Proteomics in neural crest cell research -- Neural crest methodologies in zebrafish and medaka -- Fluorescence-activated cell sorting and nanostring profiling of single neural crest cells and pigment cells -- Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity -- Avenues for investigating the neural crest and its derivatives in non-model (unconventional) vertebrates: a craniofacial skeleton perspective
This detailed book provides a wide range of techniques, from those that have been used extensively since the very first investigations into neural crest cells to those that are currently cutting-edge, in order to explore the development of neural crest cells in human, mice, rat, chick, quail, medaka, and shark. With a bit of imagination and adjustment, many of these methodologies can be adaptable to any species desirable for study. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neural Crest Cells: Methods and Protocols serves as an ideal reference guide for aspiring and experienced developmental biologists alike
X, 230 p. 36 illus. in color. : online resource.