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    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 8 (1987), S. 249-253 
    ISSN: 1573-5044
    Keywords: tissue culture ; micropropagation ; Lisianthus ; Eustoma grandiflorum ; Texas Bluebells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Explants of shoot tips, internodal stem sections, and leaf segments of Lisianthus, Eustoma grandiflorum (Griseb.) Schinners, ‘Dwarf Purple’ were cultured in vitro on modified Murashige and Skoog (MS) media. Explants of shoot tips and internodal stem sections developed into multiple shoots, whereas, leaf segments turned chlorotic on a medium supplemented with 3 mgl-1 benzyladenine (BA) and 0.2 mgl-1 naphthalene acetic acid (NAA). Shoot proliferation was obtained on shoot tips and leaf segments with 3 mgl-1 BA, but internodal stem sections became necrotic and died on this medium. Rooting was induced in cultures with multiple shoots by subculturing explants on a half-strength MS medium supplemented with 2 mgl-1 indole-3-acetic acid (IAA). Rooted plantlets were successfully transferred to soil.
    Type of Medium: Electronic Resource
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