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  • 1
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    BASEL U. A.: KARGER
    Call number: QZ200Z:32/41SUPPL1
    Keywords: Tumor / Chemotherapie ; LONIDAMIN / SPERMATOGENESEHEMMER
    Notes: VANCOUVER (WASH) 1982: OKTOBER.
    Pages: 123 S., ZAHLR. ILL.
    ISBN: 3-8055-3867-7
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  • 2
    ISSN: 1573-7373
    Keywords: astrocytoma ; glycolysis ; hexokinase ; Lonidamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Lonidamine (LND) has been shown to inhibit tumor aerobic glycolysis. Its effect was evaluated on several human astrocytomas at different degrees of malignancy; a correlation was found between LDN effect on lactate production and tumor malignancy: in grade I and II astrocytomas LND stimulates lactate production, while in grade III, IV and glioblastoma multiforme lactate production is inhibited. In an attempt to explain this different behaviour, hexokinase content and compartmentation was evaluated in astrocytomas from fresh operatory specimens and from cultured cells as well, observing a significative correlation between malignancy, hexokinase activity, percent of mitochondrially-bound hexokinase and LND effect. The results justify from a biochemical point of view the role of LND as a ‘non-conventional’ agent in multimodality combined treatment for malignant gliomas.
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  • 3
    ISSN: 1573-2576
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Recent studies from this laboratory have shown that a monoclonal antibody prepared against a specific epitope on α1-antitrypsin is a valuable diagnostic marker for autoimmune conditions. In the present study we have further characterized this monoclonal antibody and reassessed its diagnostic value in screening samples from patients with various autoimmune conditions. α1-Antitrypsin was micropurified from patients with selected autoimmune conditions and from normal donors. The purified α1-antitrypsin isolated. from patients with autoimmune conditions and normal donors was deglycosylated losing both a mixture of exoglycosidases and endoglycosidase F. The immunoreactivity of the native and deglycosylated α1-antitrypsin was examined using both a monoclonal antibody and a polyclonal antibody in enzyme linked immunosorbent assay (ELISA) and radioimmunoassay (RIA), respectively. It was noted that α1-antitrypsin isolated from patients with autoimmune diseases generated a displacement curve dissimilar to α1-antitrypsin purified from normal donors or α1 antitrypsin from patients with autoimmune diseases subjected to deglycosylation when these samples were examined by ELISA using the monoclonal antibody. However, when the polyclonal antibody was used for these studies, no difference was found between the native and deglycosylated ga1,-antitrypsin suggesting that the monoclonal antibody recognized an epitope not detectable by the polyclonal antibody. We have also assessed the diagnostic usefulness of this monoclonal antibody using a battery of 530 serum samples obtained from patients with different autoimmune diseases and compared to normal human serum (NHS,N−66); these include: systemic lupus erythematosus (SLE,N=149), rheumatoid arthritis (RA,N=64), renal diseases (NP,N=33), liver diseases (HP,N=33), mixed connective tissue disease (MCTD,N = 12), diabetes (DB,N=40), SjÖgren's syndrome (SS,N = 41), polymyositis (PM,N=20), scleroderma (SCL,N=20), Alzheimer's disease (AZ,N=11), and patients with elevated levels of carcinoembryonic antigen (CEA,N=41). The results of this study demonstrated that this monoclonal antibody is positively correlated with SLE and SS. The significance of the monoclonal antibody in connection with the pathogenesis of autoimmune diseases was discussed.
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  • 4
    ISSN: 1573-2576
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Previous studies from this and other laboratories have shown that abnormal glycosylation of several acute-phase proteins can be detected in various pathological conditions including autoimmune diseases. In the present study, we have investigated if abnormal glycosylation is limited to acute-phase proteins. We used the concanavalin A (Con A) blots in conjunction with the peptide mapping techniques to analyze serum samples and cerebrospinal fluids (CSF) obtained from patients with autoimmune diseases: systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), mixed connective tissue disease (MCTD), scleroderma (SCL), Sjögren's syndrome (SS), and polymyositis (PM); diseases of probable autoimmune origin: hepatopathies (HP); diseases of suspected autoimmune origin: schizophrenia and Alzheimer's disease (AZ); and conditions not related to autoimmunity: pregnancy (PG) and elevation of the carcinoembryonic antigen (CEA), in comparison to normal donors (NHS). We have micropurified two human proteins;α 2-macroglobulin, a non-acute-phase protein, andΒ-chain of haptoglobin, a known acute-phase protein, from serum samples of individual patients with SLE, RA, MCTD, SCL and SS, and from PG and NHS for analysis. The identity of the purified proteins was confirmed by immunoblots using either monospecific polyclonal or monoclonal antibodies, and by directN-terminal amino acid sequencing. Peptide maps for each of these proteins were generated usingStaphylococcus aureus protease V8, a Glu-C endopeptidase. When the peptide fragments ofα 2-macroglobulin were resolved by SDS-PAGE and visualized using silver staining, no differences were noted between patient samples and controls. However, when they were examined by lectin blots using Con A, the Con A-reactive fragments increased specifically and significantly in samples derived from patients of SLE, SCL, MCTD, and RA. Similarly when the peptide fragments of theΒ-chain of haptoglobin were visualized by silver staining, no differences were noted; however, the Con A reactivity of specific fragments increased in SLE, RA, SCL, and SS patients. Analysis of these results indicated that there has been a selective increase in Con A-reactive fragments in both acute-phase and non-acute-phase proteins in autoimmune conditions. Thus, the study of changes in glycosylation patterns in selected serum proteins may be a valuable diagnostic approach to define the pathophysiology of inflammatory and autoimmune disorders.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Psychopharmacology 95 (1988), S. S1 
    ISSN: 1432-2072
    Keywords: Trazodone ; Depression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Trazodone and tricyclics share similar activity towards the core symptoms of depression, whereas their effects on neurohumoral transmission tend to be opposite. This once again casts doubt upon the theories on depression postulated from the study of monoamine oxidase inhibitors and tricyclics. The effects of trazodone and tricyclics on the autonomic nervous system are also different, reflecting, respectively, the alpha-adrenergic blocking activity of the former and the muscarinic-anticholinergic and alpha-adrenergic stimulating properties of the latter. It is stressed that the autonomic changes that accompany depression to some extent overlap those produced by tricyclics, whereas they are generally relieved by trazodone. This drug, therefore, extends the previous limitations of antidepressant treatment.
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  • 6
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature medicine 12 (2006), S. 1323-1328 
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Throughout spermatogenesis, developing germ cells remain attached to Sertoli cells via testis-specific anchoring junctions. If adhesion between these cell types is compromised, germ cells detach from the seminiferous epithelium and infertility often results. Previously, we reported that Adjudin is ...
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  • 7
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1520-4804
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Inflammation research 17 (1986), S. 329-337 
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In contrast to the approach focusing on aspirin and cortisone as models for research, a physiopathologically-oriented approach provides the rationale for developing animal models suitable for detecting anti-inflammatory agents with a profile of therapeutic and side effects unlike that of currently used drugs. The so-called ‘primary’ anti-inflammatory agents have a marked efficacy in animal models of acute inflammation and lack significant anti-PG effects. Clinically, they relieve the symptoms of acute inflammatory conditions, both topically and systemically, are practically inactive in rheumatic disorders and have a profile of side-effects different from that of aspirin or cortisone. Available data suggest that their characteristic profile of side and therapeutic effects reflect qualitative, rather than quantitative differences, from aspirin and cortisone. The so-called ‘secondary’ anti-inflammatory agents affect the conditioning factors for some inflammatory diseases, including rheumatoid arthritis, rather than the inflammatory process itself. Besides the derangement of the immune system and the consequent development of immunomodulators, the role of specific protein changes as a conditioning factor is discussed and animal models are illustrated focusing on this phenomenon. The possibility is also discussed that protein denaturation is not only responsible for the formation of new antigenic determinants, but also for necrotic lesions accompanying some inflammatory disorders. Results obtained with animal models of conditioned inflammation with marked necrotic lesions are presented. The interest for this approach is that conditioning factors for inflammation appear a more specific target for drug treatment, rather than inflammation itself.
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  • 10
    ISSN: 1442-2042
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract Background: Both urinary and biliary stones can contain calcium. Bile salts (BA), which are known to bind Ca2+, are commonly used to dissolve the latter but not the former.Methods: The effect of physiologic BA on calcium oxalate (CaOx) precipitation was evaluated by a recently developed method.Results: The Ca2+ binding properties of BA were confirmed by small but significant decreases in pH observed following addition of CaCl2 to bile acids solutions. More importantly, BA inhibited CaOx precipitation with effective concentrations of approximately 10−3 mol/L. The clinical relevance of the latter observation is presently unknown but it is of note that in the same in vitro assay, the activity of BA appeared comparable to that of citric acid, the most common drug for urolithiasis. Although BA do not reach mmol/L levels in urine, they are known to change the physicochemical properties of this fluid, possibly slowing down the crystal growth process. However, the hypothetical therapeutic use of BA in former stone patients would present at least two major problems: (i) hepatotoxicity and (ii) lithogenic activity, due to hyperoxaluria subsequent to increased intestinal absorption of oxalate.〈section xml:id="abs1-1"〉〈title type="main"〉ConclusionThe ability of BA in effectively binding calcium ions and in inhibiting the precipitation of CaOx appears of interest from both a physiopathologic and a pharmacologic point of view, even if it does not currently seem exploitable for prophylactic or therapeutic purposes.
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