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  • 1
    Keywords: Botany ; Plant Sciences ; Springer eBooks
    Description / Table of Contents: Repurposing macromolecule delivery tools for plant genetic modification in the era of precision genome engineering -- The use of an automated platform to assemble multigenic constructs for plant transformation -- Ensifer-mediated transformation (emt) of rice (monocot) and oilseed rape (dicot) -- Setaria viridis as a model plant for functional genomic studies in C4 crops -- Transient transformation using particle bombardment for gene expression analysis -- Maize transformation using the morphogenic genes babyboom and wuschel2 -- Efficient and fast production of transgenic rice plants by agrobacterium-mediated transformation -- Protocol for Agrobacterium-mediated transformation and transgenic plant production of switchgrass -- Biolistic transformation of wheat -- Mesophyll protoplasts and PEG-mediated transfections: Transient assays and generation of stable transgenic canola plants -- A Unified Agrobacterium-mediated transformation protocol for alfalfa (Medicago sativa l.) and medicago truncatula -- Poplar transformation -- The genetic transformation of sweet orange (Citrus sinensis L. Osbeck) for enhanced resistance to citrus canker -- Genetic modification of grapevine embryogenic culture -- Agrobacterium-mediated transformation of Solanum tuberosum L., potato -- Agrobacterium tumefaciens-mediated transformation of tomato -- DNA break repair in plants and its application for genome engineering -- Gene stacking in plants through the application of site-specific recombination and nuclease activity -- CRISPR/Cas9 for mutagenesis in rice -- Plant biotechnology applications of zinc fingers technology -- Overview of biotechnology-derived herbicide tolerance and insect resistance traits in plant agriculture -- Developing transgenic agronomic traits for crops: targets, methods and challenges -- Transgenic and genome editing approaches for modifying plant oils -- Summary of molecular analysis methods for characterizing transgenic events -- Detection of transgenic proteins by immunoassays -- Systematic evaluation of field crop performance using modern phenotyping tools and techniques
    Abstract: This book provides thorough coverage of transgenic plants with methods on plant transformation, biotechnological application of transgenic plants, and future developments. Chapters are grouped into sections focusing on transformation model and crop plants, genome engineering, and transgenic event characterization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Transgenic Plants: Methods and Protocols aims to broaden the utility for readers, provide additional references for further understanding, and present the technology’s potential for solving some of our most urgent global challenges in food security
    Pages: XIV, 444 p. 77 illus., 73 illus. in color. : online resource.
    ISBN: 9781493987788
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  • 2
    ISSN: 1573-1561
    Keywords: Hydrocarbons ; honey bees ; insects ; Hymenoptera ; mating ; natural mating ; instrumental insemination ; communication ; pheromones ; exocrine glands ; Apidae ; gas chromatography ; chemical communication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In a series of husbandry and stop-time chemical experiments with honey bee queens, the production of tergal gland alkenes was found to be stimulated by natural mating and not by instrumental insemination. Carbon dioxide, physical manipulation of the sting chamber and vagina, presence of sperm in the spermatheca, egg production, and chemicals transferred via drone semen are demonstrated to not initiate the synthesis of the tergal gland alkenes. The compounds probably do not function as sex pheromones. However, the circumstances and timing of the initiation of production of the tergal gland alkenes strongly suggests a communication role for the compounds within the hive.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-3572
    Keywords: chronic low back pain ; Quality of Well-Being Scale ; pain assessment ; quality of life
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Outcome measures that assess quality of life for use in health policy decisions need to be investigated in chronic pain patients. In the present study, the validity of the Quality of Well-Being Scale (QWB) was evaluated on 67 adult chronic low back pain (CLBP) patients who were enrolled in a 12-week multidisciplinary pain treatment program. Participants completed the QWB, a battery of pain measures, a behavioral observation task, and a medical exam. The findings indicated that CLBP patients have a low level of functioning or quality of life (M = .567, SD = .08) compared with persons with life-threatening diseases. The QWB score was significantly correlated with observational measures of pain behavior and pain-related coping strategies. Multivariate analysis indicated that interference in daily activities, distorted ambulation, affective distress, pain duration, and guarding were the most significant predictors of quality of well-being (multiple R = .84, p < .0001). Patients with medically incongruent physical signs had significantly lower QWB scores than patients with congruent signs. Overall, the data supported the validity of the QWB in a sample of CLBP patients.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-7225
    Keywords: coumestrol ; isoflavones ; lignans ; nutrient database ; phytoestrogens ; soy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background and objective: Phytoestrogens (weak estrogens found in plants or derived from plant precursors by human metabolism) have been hypothesized to reduce the risk of a number of cancers. However, epidemiologic studies addressing this issue are hampered by the lack of a comprehensive phytoestrogen database for quantifying exposure. The purpose of this research was to develop such a database for use with food-frequency questionnaires in large epidemiologic studies. Methods: The database is based on consumption patterns derived from semistructured interviews with 118 African-American, Latina, and white women residing in California's San Francisco Bay Area. HPLC-mass spectrometry was used to determine the content of seven specific phytoestrogenic compounds (i.e. the isoflavones: genistein, daidzein, biochanin A, and formononetin; the coumestan: coumestrol; and the plant lignans: matairesinol and secoisolariciresinol) in each of 112 food items/groups. Results: Traditional soy-based foods were found to contain high levels of genistein and daidzein, as expected, as well as substantial amounts of coumestrol. A wide variety of "hidden" sources of soy (that is, soy protein isolate, soy concentrate, or soy flour added to foods) was observed. Several other foods (such as various types of sprouts and dried fruits, garbanzo beans, asparagus, garlic, and licorice) were also found to be substantial contributors of one or more of the phytoestrogens analyzed. Conclusions: Databases, such as the one described here, are important in assessing the relationship between phytoestrogen exposure and cancer risk in epidemiologic studies. Agencies, such as the United States Department of Agriculture (USDA), that routinely provide data on food composition, on which epidemiologic investigations into dietary health effects are based, should consider instituting programs for the analysis of phytochemicals, including the phytoestrogens.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: We investigated the properties of the novel dorsal root ganglion (DRG) hybrid cell line F-11 to see how closely these cells resembled normal DRG cells. Under normal growth conditions, F-11 cells appeared to contain several short neurite-like processes. However, these cells could also be grown under conditions in which they showed a much more extensive neuronal morphology, exhibiting many long neurites. Several differentiated features of DRG cells were present on F-11 cells. These included the presence of δ5-opioid receptors, receptors for prostaglandins and brady-kinin, and dihydropyridine-sensitive calcium channels. F-11 cells also synthesized and released a substance P-Iike compound, as determined by immunoreactivity. Both the number of bradykinin receptors and the voltage-sensitive calcium influx increased on cell differentiation. Opioid agonists (δ-specificity) were found to decrease cyclic AMP levels in F-l 1 cells in a naloxone- and pertussis toxin-reversible fashion. Bradykinin stimulated the synthesis of inositol-1,4-bisphosphate and inositol-1,4,5-trisphosphate. Ca2+ channel agonists stimulated voltage-sensitive Ca2+ influx in a dose-dependent, stereospecific manner, whereas Ca2+ channel antagonists inhibited Ca2+ influx. F-ll cells should, therefore, prove useful as models for authentic DRG neurons.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-7373
    Keywords: malignant gliomas ; immunotherapy ; tumor infiltrating lymphocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A prospective pilot study was performed in order to assess the safety of treating recurrent malignant gliomas (MGs) with locally infused autologous tumor infiltrating lymphocytes (TILs) and recombinant interleukin-2 (rIL-2). Six patients were entered between June 27, 1994 and June 2, 1995 and followed until July 1, 1998. At surgery an Ommaya reservoir was placed for later infusion of TILs and rIL-2. Following surgery, autologous TILs were expanded in vitro in the presence of rIL-2 and infused on treatment days 1 and 14, with concurrent rIL-2 infusions performed three times each week for one month. Following completion of immunotherapy all patients were offered chemotherapy. Phenotypic analysis demonstrated TILs to be T-lymphocytes (87–99% CD3+). Of these, 4 of 6 cases (67%) phenotyped as cytotoxic/suppressor T-lymphocytes (CD8+) and 2 of 6 cases (33%) phenotyped as helper/inducer T-lymphocytes (CD4+). TILs demonstrated limited selective cytotoxicity, with dose dependent cytotoxicity against autologous tumor, allogenic tumor and long term MG cell lines. There were no significant (Grade 3 or 4) complications. One patient developed transient low grade fevers, and 2 developed asymptomatic hydrocephalus. All patients developed transient and asymptomatic cerebral swelling, noted on the immediate post-treatment imaging studies. At three and six month follow-up, 3 patients responded with partial response, 2 demonstrated stable disease and 1 patient progressed. At long term follow-up, 1 patient had a complete response (45 month follow-up), 2 had a partial response (48 and 47 month follow-up) and 3 patients expired as a result of progressive disease (at 12, 12 and 18 months following immunotherapy). A relationship between subsequent chemotherapy or extent of resection to outcome was not appparent but could not be excluded. This pilot study demonstrated that locally infused autologous TILs and rIL-2 could be delivered without serious toxicity. Further studies are indicated to determine the safety and long term efficacy of TIL immunotherapy.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The introduction of robotic and computer technology into surgical operations allows dexterity to be increased and surgical procedures to be carried out from a distance (telesurgery). But until now, the distance feasible for remote telesurgery was considered to be limited to a few hundred miles ...
    Type of Medium: Electronic Resource
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  • 8
    Publication Date: 2017-05-27
    Description: Blue spaces (water bodies) may promote positive mental and physical health through opportunities for relaxation, recreation, and social connections. However, we know little about the nature and extent of everyday exposure to blue spaces, particularly in settings outside the home or among children, nor whether exposure varies by individual or household characteristics. Wearable cameras offer a novel, reliable method for blue space exposure measurement. In this study, we used images from cameras worn over two days by 166 children in Wellington, New Zealand, and conducted content and blue space quantification analysis on each image (n = 749,389). Blue space was identified in 24,721 images (3.6%), with a total of 23 blue recreation events. Visual exposure and participation in blue recreation did not differ by ethnicity, weight status, household deprivation, or residential proximity to the coastline. Significant differences in both visual exposure to blue space and participation in blue recreation were observed, whereby children from the most deprived schools had significantly higher rates of blue space exposure than children from low deprivation schools. Schools may be important settings to promote equitable blue space exposures. Childhood exposures to blue space may not follow the expected income inequality trends observed among adults.
    Print ISSN: 1661-7827
    Electronic ISSN: 1660-4601
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Published by MDPI Publishing
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