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  • 1
    Keywords: RECEPTOR ; CELLS ; CELL ; Germany ; human ; MICROSCOPY ; NEW-YORK ; HYBRIDIZATION ; PROTEIN ; PROTEINS ; ACTIVATION ; kidney ; IN-SITU ; immunohistochemistry ; UP-REGULATION ; COMPONENT ; PCR ; EPITHELIAL-CELLS ; POLYMERASE-CHAIN-REACTION ; immune response ; IMMUNE-RESPONSE ; CHAIN-REACTION ; REJECTION ; SEGMENTS ; LOCATION ; TNF-ALPHA ; in situ hybridization ; CYTOKINE ; CHAIN ; secretion ; polymerase chain reaction ; BLOOD MONONUCLEAR-CELLS ; INTERCELLULAR-ADHESION MOLECULE-1 ; USA ; immunology ; distal tubule ; ACUTE TUBULAR-NECROSIS ; collecting ducts ; CONNECTING TUBULE ; CYTOKINE RESPONSES ; GAMMA-INDUCING FACTOR ; INTERLEUKIN-18
    Abstract: We determined the cellular location of interleukin-18 (IL-18) and caspase-1 and the purinergic receptor P2X7, two proteins necessary for its activation and secretion. The mRNA and protein of IL-18 were detectable in normal human kidney by means of polymerase chain reaction (PCR), in situ hybridization, and Western blot. Immunohistochemistry located IL-18 to nephron segments containing calbinbin-D28k or aquaporin-2 that suggest location in the distal convoluted and the connecting tubule and to parts of the collecting duct. IL-18 was not detected in the thick ascending limb of Henle. Confocal microscopy showed that IL-18 was expressed in cells negative for calbindin-D28k and for aquaporin-2 but positive for the vacuolar H+ -ATPase. This demonstrates that the intercalated cells produce IL-18. These segments were also positive for caspase-1 and P2X7 that are essential for IL-18 secretion. Our results show that IL-18 is constitutively expressed by intercalated cells of the late distal convoluted tubule, the connecting tubule, and the collecting duct of the healthy human kidney. Since IL-18 is an early component of the inflammatory cytokine cascade, its location suggests that renal intercalated cells may contribute to immediate immune response of the kidney
    Type of Publication: Journal article published
    PubMed ID: 17687255
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  • 2
    Keywords: ADAM10, ALPHA, BIOPSY, CELL, CELL SUBSETS, CELLS, chemokine, CHEMOKINE LIGAND-16, CXCL16, DAMAGE, DI
    Abstract: The chemokine CXCL16 plays an important role in the recruitment of leukocytes to sites of inflammation influencing the course of experimental glomerulonephritis. Here we show that human kidneys highly express CXCL16 in the distal tubule, connecting tubule and principal cells of the collecting duct with weak expression in the thick ascending limb of Henle. Beside the membrane localization, a soluble form of CXCL16 can be proteolytically released which acts as a chemotactic factor. In human renal tissue the expression pattern of the disintegrin-like metalloproteinase ADAM10 is similar to that of CXCL16, suggesting ADAM10 can potentially cleave CXCL16 in vivo. When we tested this in primary tubular cells we found that blockade of ADAM10 activity inhibited the IFN-gamma induced release of soluble CXCL16. Acute tubular damage in renal allografts was associated with elevated urinary CXCL16 and this correlated with focally increased apical CXCL16 expression in the distal tubules and collecting ducts. Renal allograft biopsies, with a histopathological diagnosis of acute interstitial rejection, showed increased basolateral ADAM10 expression together with high numbers of infiltrating T cells. Our results suggest that CXCL16 and ADAM10 are involved in the recruitment of T cells to the kidney and play an important role in inflammatory kidney diseases
    Type of Publication: Journal article published
    PubMed ID: 18480749
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  • 3
    ISSN: 1600-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Cumulative caries prevalences were estimated clinically in yearly cohorts of boys and girls, between 1 and 7 years of age, with at least 150 examinees in each cohort, and radiographically also from 4 years of age, in the occlusal, mesial, and distal surfaces of all four pairs of primary molars. Analysis of caries prevalence curves revealed best fit (based on least squares) with a transformed arc tan function with left end cut off, both for occlusal and for proximal surface caries. Compared with the exponential function, fit with the arc tan function was better in 19 out of a total of 24 comparisons. This difference was statistically significant in eight instances, but only in one instance of the reverse finding. By mathematical description of cumulative caries prevalence, no essential differences were revealed in the growth pattern of caries in occlusal and proximal surfaces of the primary molars. Implications of this finding are discussed.
    Type of Medium: Electronic Resource
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