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  • 1
    Keywords: Life sciences ; Stem Cells ; Regenerative Medicine ; Tissue Engineering ; Developmental Biology ; Life sciences ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Developmental Biology ; Springer eBooks
    Description / Table of Contents: Preface -- 1 Generation of various telencephalic regions from human embryonic stem cells in three dimensional culture -- 2 Self-organized cerebellar tissue from human pluripotent stem cells and its application to clinical medicine -- 3 Functional Pituitary Tissue formation Recapitulating hypothalamus and pituitary development using ES/iPS cells -- 4 Inner Ear Organoids: Recapitulating Inner Ear Development in 3D Culture -- 5 Functional Tooth Regeneration -- 6 Functional Hair Follicle Regeneration -- 7 Functional salivary gland regeneration -- 8 Functional Lacrimal Gland Regeneration -- 9 Generation of Gastrointestinal Organoids derived from Human Pluripotent Stem Cells -- 10 Early kidney specification and its recapitulation by pluripotent stem cells -- 11 Recapitulating development to generate kidney organoid cultures -- 12 Liver Regeneration using Cultured Liver Bud -- 13 Development and Bioengineering of Lung Regeneration
    Abstract: This book reviews three-dimensional (3D) stem cell culture and proof of concept for organ regeneration. The chapters present studies based on developmental biology but not tissue engineering using bio-degradative scaffolds. The ultimate goal of regenerative therapy, the next generation of regenerative therapy, is to develop fully functioning bioengineered 3D organs that can replace lost or damaged organs following disease, injury, or aging. Next-generation regenerative therapy will consist of organ-replacement regenerative therapy, which aims to reproduce reciprocal epithelial and epithelial interactions and epithelial and mesenchymal interactions that occur during embryogenesis. The book then discusses the generation of several 3D functional organs and organoids such as brain, inner ear, tooth, hair, salivary glands, lacrimal glands, gastrointestinal organs, kidney, liver, and lung. This work will appeal to a wide readership such as medical scientists, developmental biologists, clinicians, and patients. The volume provides valuable information and ideas to form a next-generation field of science
    Pages: XIII, 257 p. 67 illus., 64 illus. in color. : online resource.
    ISBN: 9789811037689
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  • 2
    Keywords: Life sciences ; Human Genetics ; Health Informatics ; Bioinformatics ; Biostatistics ; Life sciences ; Bioinformatics ; Human Genetics ; Biostatistics ; Health Informatics ; Springer eBooks
    Description / Table of Contents: Drug Discovery -- Translational Bioinformatics and Drug Discovery -- Translational Research in Drug Discovery and Development -- Exploring the Potential of Herbal Ligands towards Multidrug Resistant Bacterial Pathogens by Computational Drug Discovery -- The Progress of New Targets of Anti-HIV and Its Inhibitors -- Exploration of Drug Candidates Interacting on Amyloid-β Protofibrils for Alzheimer’s Disease -- Homology Modeling, Structure Based Pharmacophore Modeling, High Throughput Virtual Screening and Docking Studies of L-Type Calcium Channel for Cadmium Toxicity -- Natural Compounds Are Smart Player in Context to Anticancer Potential: An In Silico and In Vitro Advancement -- Genome Wide Association Studies -- A Survey of Bioinformatics Based Tools in RNA-Sequencing (RNA-Seq) Data Analysis -- Epigenetics and Its Role in Human Cancer -- Methods for Microbiome Analysis -- Pharmacogenomics: Clinical Perspective, Strategies and Challenges -- Computational Network Approaches and Their Applications for Complex Diseases -- Bioinformatics Applications in Clinical Microbiology -- Artificial Intelligence and Automatic Image Interpretation in Modern Medicine -- Computation in Medicine: Medical Image Analysis and Visualization
    Abstract: This book offers a detailed overview of translational bioinformatics together with real-case applications. Translational bioinformatics integrates the areas of basic bioinformatics, clinical informatics, statistical genetics and informatics in order to further our understanding of the molecular basis of diseases. By analyzing voluminous amounts of molecular and clinical data, it also provides clinical information, which can then be applied. Filling the gap between clinic research and informatics, the book is a valuable resource for human geneticists, clinicians, health educators and policy makers, as well as graduate students majoring in biology, biostatistics, and bioinformatics
    Pages: VIII, 437 p. 85 illus., 59 illus. in color. : online resource.
    ISBN: 9789402410457
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  • 3
    Keywords: Medicine ; Human Physiology ; Human Anatomy ; Gynecology ; Biomedicine ; Human Physiology ; Gynecology ; Anatomy ; Springer eBooks
    Description / Table of Contents: The Pelvis or My Pelvis -- “Construction Plan” of the Bony Pelvis -- The Pelvis at War -- The Birth Canal -- History -- Concepts and Approaches in the Study of the Pelvis -- Sexual Organs and Society -- Anatomy of the Pelvic Wall -- The Development of Uropoetic Structures -- Physiotherapy for Pelvic Muscles -- The Sub and Retroperitoneal Space -- Vision and Waist-to-Hip Ratio -- Male and Female Bladder -- The Pelvis and Herbal Medicine -- Innervation of the Mature Human Pelvis -- Electromyography of Smooth Muscle of Bladder and Uterus -- Reflexes or Controller Action? -- The Connective Tissue in the Pelvis -- Sitting a Pelvic Function? -- Sphincters -- Catheters -- The Pelvis During Childhood and Puberty -- The Pelvis and Aging
    Abstract: This book offers a critical review of the pelvic scienceś€”past, present and futur途from an anatomical and physiological perspective and is intended for researchers, medical practitioners and paramedical therapists in the fields of urology, gynecology and obstetrics, proctology, physiotherapy, as well as for patients. ℗ The book starts with a ́€œconstruction plań€ of the pelvis and shows its structural consequences. The historical background of pelvic studies proceeds from medieval and early Italian models to the definitive understanding of the pelvic anatomy in the Seventeenth century. During these eras of pelvic research, concepts and approaches developed that are illustrated with examples from comparative anatomy and from mutations, also with regard to the biomechanics of pelvic structures. Perceptions of the pelvis as an important element in sexual arousal and mating conduct are discussed, as well as attitudes to circumcision, castration and other mutilations, in its anthropological, social context. ℗ The anatomy and physiology of the pelvic wall and its organs as well as the development of these pelvic organs are covered as a prerequisite to understanding, for example, the spread of pelvic carcinoma and male and female bladder muscle function. Connective pelvic tissue is examined in its reinforcing capacity for pelvic structures, but also as a ́€œhiding plac逝 for infections. Innervations and reflexes relayed through the pelvic nerves are discussed in order to explain incontinence, sphincter function and the control of smooth and striated muscles in the pelvis. ℗ Catheters and drugs acting on pelvic function are described, and a critical review of alternative clinical methods for treating pelvic dysfunctions is provided
    Pages: XVII, 558 p. 203 illus., 80 illus. in color. : online resource.
    ISBN: 9783642400063
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  • 4
    Keywords: Medicine ; Toxicology ; Enzymes ; Biomedicine ; Pharmacology/Toxicology ; Enzymology ; Springer eBooks
    Description / Table of Contents: Enzyme Kinetics in Drug Metabolism – Fundamentals and Applications -- Fundaments of Enzyme Kinetics -- Different Enzyme Kinetic Models -- Reversible Mechanisms of Enzyme Inhibitors and Resulting Clinical Significance -- Irreversible Enzyme Inhibition Kinetics and Drug-Drug Interactions -- Multi-Enzyme Kinetics and Sequential Metabolism -- Consideration of the Unbound Drug Concentration in Enzyme Kinetics -- Enzyme Kinetics of Oxidative Metabolism – Cytochromes P450 -- Enzyme Kinetics, Pharmacokinetics, Inhibition, and Regioselectivity of Aldehyde Oxidase -- Enzyme Kinetics of Conjugative Enzymes – PAPS -- Enzyme Kinetics of Uridine Diphosphate Glucuronosyltransferases (UGTs) -- Principles and Experimental Considerations for in vitro Transporter Interaction Assays -- Rationalising Under-Prediction of Drug Clearance from Enzyme and Transporter Kinetic Data – from in vitro Tools to Mechanistic Modeling -- The Structural Model for the Mass Action Kinetic Analysis of P-gp Mediated Transport Through Confluent Cell Monolayers -- Systems Biology Approaches to Enzyme Kinetics: Analyzing Network Models of Drug Metabolism -- Variability in Human in vitro Enzyme Kinetics -- Sources of Interindividual Variability -- Case Study 1. Practical Considerations with Experimental Design and Interpretation -- Case Study 2. Practical Analytical Considerations for Conducting in vitro Enzyme Kinetic Studies -- Case Study 3. Application of Basic Enzyme Kinetics to Metabolism Studies – Real Life Examples -- Case Study 4. Predicting the Drug Interaction Potential for Inhibition of CYP2C8 by Montelukast -- Case Study 5. Deconvoluting Hyperbilirubinemia: Differentiating Between Hepatotoxicity and Reversible Inhibition of UGT1A, MRP2 or OATP1B1 in Drug Development -- Case Study 6. Drug Transporters: in vitro Solutions for Translatable Outcomes -- Case Study 7. Compiled Aha Moments in Enzyme Kinetics: Authors’ Experiences
    Abstract: Drug metabolism and transport are very important facets within the discipline of pharmaceutical sciences, with ℗ enzyme kinetic concepts utilized regularly in characterizing and modeling the disposition and elimination of drugs. Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications focuses on very practical aspects of applying kinetic principles to drug metabolizing enzymes and transporters. Divided into five convenient sections, topics include the fundamental principles of enzyme kinetics, the kinetics of oxidative and conjugative drug metabolizing enzymes and drug transporters, modeling approaches for both drug metabolizing enzymes and transporters including novel systems biology approaches, understanding of variability both experimental and interindividual (pharmacogenomic), and case studies that provide real life examples of applying these principles. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics especially suitable for the novice, in some cases step-by-step, readily reproducible protocols, and insights to help with troubleshooting and avoiding known pitfalls with extensive cross referencing to assist in learning. ℗ Authoritative and easily accessible, Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications serves as a very practical teaching tool for novice, non-mathematically trained scientists interested in these fundamental concepts and as an aid for their supervisors in teaching these principles.℗ ℗
    Pages: XVIII, 538 p. 134 illus., 36 illus. in color. : online resource.
    ISBN: 9781627037587
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  • 5
    Keywords: Medicine ; Human Genetics ; Medical genetics ; Biomedicine ; Human Genetics ; Gene Function ; Springer eBooks
    Description / Table of Contents: RecTEPsy Mediated Recombineering in Pseudomonas syringae -- Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophila -- Multiple Genetic Manipulations of DT40 Cell Line -- Gene Targeting of Human Pluripotent Stem Cells by Homologous Recombination -- Methods for the Assessment of ssODN-Mediated Gene Correction Frequencies in Muscle Cells -- Small Fragment Homologous Replacement (SFHR): Sequence-Specific Modification of Fenomic DNA in Eukaryotic Cells by Small DNA Fragments -- Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotide Donors for Gene Correction -- Triplex-Mediated Genome Targeting and Editing -- Targeting piggyBac Transposon Integrations in the Human Genome -- Gene Targeting in Human Induced Pluripotent Stem Cells with Adenoviral Vectors -- Enhanced Gene Targeting of Adult and Pluripotent Stem Cells Using Evolved Adeno-Associated Virus -- Lentiviral Vectors Encoding Zinc-Finger Nucleases Specific for the Model Target Locus HPRT1 -- Designing and Testing the Activities of TAL Effector Nucleases -- A Bacterial One-Hybrid System to Isolate Homing Endonuclease Variants with Altered DNA Target Specificities -- Design and Analysis of Site-Specific Single-Strand Nicking Endonucleases for Gene Correction -- CRISPR-Cas Mediated Targeted Genome Editing in Human Cells -- RNA-Guided Genome Editing of Mammalian Cells -- Nuclease-Mediated Double Strand Break (DSB) Enhancement of Small Fragment Homologous Recombination (SFHR) Gene Modification in Human Induced Pluripotent Stem Cells (hiPSCs) -- AAV-Mediated Gene Editing via Double-Strand Break Repair -- Genetic Modification Stimulated by the Induction of a Site-Specific Break Distant from the Locus of Correction in Haploid and Diploid Yeast Saccharomyces cerevisiae -- A Southern Blot Protocol to Detect Chimeric Nuclease-Mediated Gene Repair -- High-Throughput Cellular Screening of Engineered Nuclease Activity Using the Single-Strand Annealing Assay and Luciferase Reporter -- An Unbiased Method for Detection of Genome-Wide Off Target Effects in Cell Lines Treated with Zinc Finger Nucleases -- Identification of Off-Target Cleavage Sites of Zinc Finger Nucleases and TAL Effector Nucleases Using Predictive Models -- Method for Retinal Gene Repair in Neonatal Mouse -- In utero Delivery of Oligodeoxynucleotides for Gene Correction -- Portal Vein Delivery of Viral Vectors for Gene Therapy for Hemophilia -- Gene Correction of Induced Pluripotent Stem Cells Derived from a Murine Model of X-Linked Chronic Granulomatous Disorder -- Efficient Transduction of Hematopoietic Stem Cells and its Potential for Gene Correction of Hematopoietic Diseases
    Abstract: Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ℗ Authoritative and easily accessible, Gene Correction: Methods and Protocols seeks to serve scientists of all backgrounds interested in the area of gene targeting/recombination/therapy
    Pages: XVI, 463 p. 87 illus., 59 illus. in color. : online resource.
    ISBN: 9781627037617
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  • 6
    Keywords: Life sciences ; Cytology ; Developmental Biology ; Laboratory animals ; Life sciences ; Cell Biology ; Developmental Biology ; Animal Models ; Springer eBooks
    Description / Table of Contents: Culturing Larvae of Marine Invertebrates -- Obtaining Gametes and Embryos of Ascidians -- Artificial Seawater Culture of the Gastropod Crepidula fornicata for Studies of Larval Settlement and Metamorphosis -- Trichoplax adhaerens, an Enigmatic Basal Metazoan with Potential -- Rearing Gymnolaemate Bryozoan Larvae for Cellular and Molecular Analyses -- An Invertebrate Embryologist's Guide to Routine Processing of Confocal Images -- TEM Analyses of Chaetognath Reproductive Organs -- SEM Analysis of Marine Invertebrate Gametes -- Imaging Neural Development in Embryonic and Larval Sea Urchins -- Preparation and Use of Sea Urchin Egg Homogenates -- Microinjection and 4D Fluorescence Imaging in the Eggs and Embryos of the Ascidian Phallusia mammillata -- Isolating Specific Embryonic Cells of the Sea Urchin by FACS -- Biotinylation of Oocyte Cell Surface Proteins of the Starfish Patiria miniata -- Perturbations to the Hedgehog Pathway in Sea Urchin Embryos -- Regulation of DNA Synthesis at the First Cell Cycle in the Sea Urchin in vivo -- Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte ℗ -- Maturation in Marine Nemertean Worms -- Multi-color Labeling in Developmental Gene Regulatory Network Analysis -- A 96́€“well Plate Format for Detection of Marine Zooplankton with the Sandwich Hybridization Assay -- Isolation and Assessment of Signaling Proteins from Synchronized Cultures During Egg Activation and Through the Egg-to-Embryo Transition in Sea Urchines -- Tools for Sea Urchin Genomic Analysis -- Antibody Inhibition of Protein Activity in Starfish Oocytes -- Immunophotoaffinity Labeling of the Binding Proteins for 1-Methyladenine, an Oocyte-maturation Inducing Hormone of Starfish.℗ ℗
    Abstract: In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Developmental Biology of the Sea Urchin and other Marine Invertebrates: Methods and Protocols seeks to aid scientists in the further study into sea urchins and other marine invertebrates
    Pages: XIV, 347 p. 69 illus., 14 illus. in color. : online resource.
    ISBN: 9781627039741
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  • 7
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: The Pre-mRNA Splicing Reaction -- Diversity and Evolution of Spliceosomal Systems -- Mechanisms of Spliceosomal Assembly -- Alternative Pre-mRNA Splicing -- Regulation of Alternative Pre-mRNA Splicing -- Introduction to Co-Transcriptional RNA Splicing -- Chromatin and Splicing -- Preparation of Splicing Competent Nuclear Extracts -- Preparation of Yeast Whole Cell Splicing Extract -- Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase -- In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract -- Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract -- In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation -- Isolation and Accumulation of Spliceosomal Assembly Intermediates -- Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function -- Expression and Purification of Splicing Proteins from Mammalian Cells -- Single Molecule Approaches for Studying Spliceosome Assembly and Catalysis -- Cell-Based Splicing of Minigenes -- Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA -- Antisense Methods to Modulate Pre-mRNA Splicing -- Using Yeast Genetics to Study Splicing Mechanisms -- Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR -- Chromatin Immunoprecipitation Approaches to Determine Co-Transcriptional Nature of Splicing -- Computational Approaches to Mine Publicly Available Databases -- Approaches to Link RNA Secondary Structures with Splicing Regulation -- Methods to Study Splicing from High-Throughput RNA Sequencing Data -- Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq -- Predicting Alternative Splicing
    Abstract: Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo, manipulation of splicing events, and high-throughput and bioinformatic analyses of alternative splicing.℗ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come
    Pages: XI, 427 p. 66 illus., 34 illus. in color. : online resource.
    ISBN: 9781627039802
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  • 8
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Preface -- Nanoscale imaging of protein molecules at the postsynaptic densit -- Electron tomography for the study of synaptic ultrastructure in fixed brain sections -- Time-resolved electron microscopy: coupling optogenetics with high-pressure freezing -- Investigating AMPA receptors diffusion and nanoscale organization at synapses with high density single molecule tracking methods -- Visualising the ultra-structures and dynamics of synapses by single molecule nanoscopy -- Live-cell PALM of intracellular proteins in neurons -- Monitoring nanoscale mobility of small molecules with time-resolved fluorescence anisotropy imaging -- Imaging signalling transduction in single dendritic spines -- Imaging-based measures of synaptic tenacity -- Zooming in on the (peri)synaptic extracellular matrix -- Two-photon STED microscopy for imaging synapses and glia in acute brain slices -- Index
    Abstract: Synapses underlie rapid and flexible neural communication in the brain and they hold the key to understanding higher brain functions in health and disease. Because they are very small and highly dynamic, it is very difficult to study them with traditional techniques. Fortunately, recent ground-breaking advances in microscopy have greatly improved our ability to image synapses at the nanoscale, even down to the level of single molecules. Authored by leading practitioners and developers in the field, this volume focuses on the nanoscale analysis of the molecular and structural organization and dynamics of synapses of the central nervous system, utilizing superresolution (e.g. PALM, STORM, STED) and other advanced methods (e.g. EM tomography, optogenetics, FLIM). It explains the basic principles behind the various nanoscale imaging modalities, how they are implemented and what their scope and limitations are, while also highlighting several exciting new research opportunities for synapse research enabled by them
    Pages: X, 231 p. 57 illus., 49 illus. in color. : online resource.
    ISBN: 9781461491798
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  • 9
    Keywords: Medicine ; Neurosciences ; Microscopy ; Biomedicine ; Neurosciences ; Biological Microscopy ; Springer eBooks
    Description / Table of Contents: Translation, Touch, and Overlap in Multi-Fluorescence Confocal Laser Scanning Microscopy to Quantitate Synaptic Connectivity -- Surgical Procedures to Study Microglial Motility in the Brain and in the Spinal Cord by In Vivo Two-Photon Laser-Scanning Microscopy -- Analysis of Brain Projection Systems Using Third-Generation Neuroanatomical Tracers and Multiple Fluorescence Laser Scanning Microscopy -- Combining Multi-Channel Confocal Laser Scanning Microscopy with Serial Section Reconstruction to Analyze Large Tissue Volumes at Cellular Resolution -- Modeling Excitotoxic Ischemic Brain Injury of Cerebellar Purkinje Neurons by Intravital and In Vitro Multi-Photon Laser Scanning Microscopy -- Analysis of Morphology and Structural Remodeling of Astrocytes -- Quantitative Analysis of Axonal Outgrowth in Mice -- Zebrafish Brain Development Monitored by Long-Term In Vivo Microscopy: A Comparison Between Laser Scanning Confocal and 2-Photon Microscopy -- Analysis of Actin Turnover and Spine Dynamics in Hippocampal Slice Cultures -- Quantitative Geometric Three-Dimensional Reconstruction of Neuronal Architecture and Mapping of Labeled Proteins from Confocal Image Stacks -- Confocal Microscopy Used for the Semiautomatic Quantification of the Changes in Aminoacidergic Fibers During Spinal Cord Regeneration -- Reconstruction and Morphometric Analysis of Hippocampal Neurons from Mice Expressing Fluorescent Proteins -- Machine Learning to Evaluate Neuron Density in Brain Sections -- Shearlet-Analysis of Confocal Laser-Scanning Microscopy Images to Extract Morphological Features of Neurons
    Abstract: Laser Scanning Microscopy and Quantitative Image Analysis of Neuronal Tissue brings together contributions from research institutions around the world covering pioneering applications in laser scanning microscopy and quantitative image analysis and providing information about the power and limitations of this quickly developing field.℗ This detailed volume seeks to introduce key questions, to provide detailed information on how to acquire data by laser-scanning microscopy, and to examine how to use the often huge digital data set in an efficient manner to extract maximum information. Thus the book not only provides a compilation of diverse protocols but aims to bring together biological bench work, laser scanning microscopy, and mathematical, computer-assisted data analysis to grasp novel insights of form, dynamics, and interactions of microscopy-sized biological objects.℗ Written in the popular Neuromethods series format, chapters include the kind of practical implementation advice that promises successful results. ℗ Wide-ranging and innovative, Laser Scanning Microscopy and Quantitative Image Analysis of Neuronal Tissue will stimulate the reader to make efficient use of the application of laser scanning microscopy for his or her own research question
    Pages: XII, 307 p. 107 illus., 84 illus. in color. : online resource.
    ISBN: 9781493903818
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  • 10
    Keywords: Medicine ; Neurosciences ; Neurobiology ; Biomedicine ; Neurosciences ; Neurobiology ; Springer eBooks
    Description / Table of Contents: Cerebral Angiogenesis During Development: Who’s Conducting the Orchestra? -- Cerebral Angiogenesis: A Realistic Therapy for Ischemic Disease? -- Vascular Normalization in Cerebral Angiogenesis: Friend or Foe? -- Pericytes and Adaptive Angioplasticity: The Role of Tumor Necrosis Factor Weak Inducer of Apoptosis (TWEAK) -- Analysis of Angiogenesis in the Developing Mouse Central Nervous System -- Hypoxia-Induced Angiogenesis and Capillary Density Determination -- The Middle Cerebral Artery Occlusion Model of Transient Focal Cerebral Ischemia -- A Mouse Model of Chronic Cerebral Hypoperfusion Characterizing Features of Vascular Cognitive Impairment -- A Mouse Model of Permanent Focal Ischemia: Distal Middle Cerebral Artery Occlusion -- A Method of Inducing Global Cerebral Ischemia -- Induction of Cerebral Arteriogenesis in Mice -- Vessel Painting Technique for Visualizing the Cerebral Vascular Architecture of the Mouse -- Examining Cerebral Angiogenesis in Response to Physical Exercise -- Histological Assessment of Angiogenesis in the Hypoxic Central Nervous System -- Examining Vascular Remodeling in the Hypoxic Central Nervous System -- Analysis of Cerebral Angiogenesis in Human Glioblastomas -- Quantitative Cerebral Blood Flow Measurements Using MRI -- Fluorescent Angiogenesis Models Using Gelfoam® Implanted in Transgenic Mice Expressing Fluorescent Proteins -- Laser Speckle Contrast Imaging to Measure Changes in Cerebral Blood Flow -- Laser Doppler Flowmetry to Measure Changes in Cerebral Blood Flow -- Defining the Role Of HIF and Its Downstream Mediators in Hypoxic-Induced Cerebral Angiogenesis -- Inducible Gene Deletion in Glial Cells to Study Angiogenesis in the Central Nervous System -- Bone Marrow Chimera Experiments to Determine the Contribution of Hematopoietic Stem Cells to Cerebral Angiogenesis -- Novel Methods for Accurate Identification, Isolation, and Genomic Analysis of Symptomatic Micro-Environments in Atherosclerotic Arteries -- Induction of Brain Arteriovenous Malformation in the Adult Mouse -- Stimulation of Cerebral Angiogenesis by Gene Delivery -- Investigating the Role of Perlecan Domain V in Post-Ischemic Cerebral Angiogenesis -- Isolation and Culture of Primary Mouse Brain Endothelial Cells -- Purification of Endothelial Cells from the Rat Brain -- Generation of Primary Cultures of Bovine Brain Endothelial Cells and Setup of Co-Cultures with Rat Astrocytes -- Isolation and Culture of Primary Pericytes from Mouse Brain -- Assays to Examine Endothelial Cell Migration, Tube Formation, and Gene Expression Profiles -- A Neurovascular Blood-Brain Barrier In Vitro Model -- In Vitro Models of the Blood-Brain Barrier
    Abstract: Providing a selection of the key techniques that are used in characterizing cerebral angiogenesis, Cerebral Angiogenesis: Methods and Protocols aims to define the cellular and molecular mechanisms underlying this important process.℗ Divided into six parts, this detailed volume examines cerebral angiogenesis occurring in different scenarios, a variety of different models in which cerebral angiogenesis can be studied, methods to characterize and quantify angiogenic events as well as several different approaches to measure changes in cerebral blood flow, different approaches to investigate the role of specific candidate genes in cerebral angiogenesis, methods of therapeutically manipulating cerebral angiogenesis by gene delivery, and finally important approaches to examine cerebral angiogenic mechanisms in vitro.℗ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Comprehensive and decidedly practical, Cerebral Angiogenesis: Methods and Protocols should be a vital resource for all researchers, both those new to this field as well as those looking to use more specialized and sophisticated techniques to examine blood vessel growth in the brain
    Pages: XV, 442 p. 102 illus., 83 illus. in color. : online resource.
    ISBN: 9781493903207
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