Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Keywords: Medicine ; Oncology ; Human Genetics ; Biomedicine ; Cancer Research ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Identification and Analysis of Precursors to Invasive Pancreatic Cancer -- Tissue Microarrays: Construction and Use -- Immunohistochemistry of Pancreatic Neoplasia -- A Method for Conducting Highly Sensitive microRNA in situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer -- Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications -- Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis -- DNA Methylation Analysis in Human Cancer -- Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer Patients -- Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer Vaccine -- The Prevention and Genetics of Pancreatic Cancer ́€“ A Programmatic Approach -- Development of Orthotopic Pancreatic Tumor Mouse Models -- Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems -- Quantification of Murine Pancreatic Tumors by High Resolution Ultrasound -- Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells -- Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells -- Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis -- Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo -- The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells -- Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods -- Evaluating Tumor Angiogenesis -- Pooled Genome-wide RNAi Screenings -- Pooled ShRNA screenings: Computational Analysis. ℗
    Abstract: Pancreatic ductal adenocarcinoma is the fifth leading cause of cancer death in the USA.℗ Pancreatic cancer develops as a result of the accumulation of genetic and epigenetic alterations in cancer-causing genes such as oncogenes and tumor-suppressor genes.℗ The second edition of Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer.℗ ℗ It covers topics from in-vitro cell cultures to in-vivo mouse models, DNA to protein manipulation, and genetic and epigenetic analyses to treatment development. Written in the highly successful Methods in Molecular Biologý„Ø series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, written by highly renowned investigators with expertise in pancreatic cancer, Pancreatic Cancer: Methods and Protocols, Second Edition is an invaluable source of proven protocols to those who are interested in joining the fight against pancreatic cancer.℗
    Pages: XII, 389 p. 53 illus., 45 illus. in color. : digital.
    Edition: 2nd ed. 2013.
    ISBN: 9781627032872
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Keywords: Oncology ; Cancer Research ; Springer eBooks
    Description / Table of Contents: Subtyping of IPMN -- Clinical Management and Surgical Decision-Making of IPMN of the Pancreas -- Develop Nomogram to Predict Malignancy of Intraductal Papillary Mucinous Neoplasm -- Generation of Induced Pluripotent Stem Cell-Like Lines from Human Pancreatic Ductal Adenocarcinoma -- Lineage Tracing of Primary Human Pancreatic Acinar and Ductal Cells for Studying Acinar to Ductal Metaplasia -- Spheroid Culture of Human Pancreatic Ductal Cells to Reconstitute Development of Pancreatic Intraepithelial Neoplasia -- Pancreatic Microtumors: A Novel D Ex Vivo Testing Platform -- 3D Models of Pancreatic Ductal Adenocarcinoma via Tissue Engineering -- Generation and Culture of Human Pancreatic Ductal Adenocarcinoma Organoids from Resected Tumor Specimens -- Generation and Culture of Tumor and Metastatic Organoids from Murine Models of Pancreatic Ductal Adenocarcinoma -- An In Vitro Three-Dimensional Organotypic Model to Analyze Peripancreatic Fat Invasion in Pancreatic Cancer: A Culture System Based on Collagen Gel Embedding -- Osteoclasts Differentiation Assay -- Primary Cultures for Pancreatic Stellate Cells (PSCs) -- Cytokine Profiling and Orthotopic Xenografing of Pancreatic Stellate Cells -- Quantitative Method to Track Proteolytic Invasion in 3D Collagen -- Detection and Quantification of Macropinosomes in Pancreatic Tumors -- Evaluating Stress Granules in Pancreatic Cancer In Vitro and In Vivo -- Methods for Monitoring Macroautophagy in Pancreatic Cancer Cells -- Measurement of Reactive Oxygen Species by Fluorescent Probes in Pancreatic Cancer Cells -- Evaluating the Metabolic Alterations in Pancreatic Cancer -- Isolation of Extracellular Vesicles for Cancer Diagnosis and Functional Studies -- Evaluation of Post-Transcriptional Gene Regulation in Pancreatic Cancer Cells: Studying RNA Binding Proteins and their mRNA Targets -- Laser Capture Microdissection on Frozen Sections for Extraction of High-Quality Nucleic Acids -- Statistical Methods for Identifying Biomarkers from miRNA Profiles of Cancers -- Induction of Pancreatic Inflammation Accelerates Pancreatic Tumorigenesis in Mice -- Pancreatic Acinar to Ductal Metaplasia and Pancreatic Cancer -- Orthotopic Pancreatic Tumor Mouse Models of Liver Metastasis -- Molecular and Physiological Evaluation of Pancreatic Cancer-Induced Cachexia
    Abstract: This third edition volume expands on the previous editions with discussions on the latest developments in 2D- and 3D-organoid cultures that allow for the execution of precision medicine, and in vitro studies of tumor cell growth and invasion. The chapters in this book explore protocols for evaluating macropinocytosis, stress granules, autophagy, metabolic alterations, and reactive oxygen species, which have showed to be possible therapeutic opportunities. The protocols described in this book look at both human and animal models, ranging from in vitro and in vivo, and will aid in future research of pancreatic cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Pancreatic Cancer: Methods and Protocols, Third Edition is a valuable resource for scientists and researchers who are interested in learning more about ways to improving patient care for pancreatic cancer
    Pages: XII, 337 p. 51 illus., 40 illus. in color. : online resource.
    Edition: 3rd ed. 2019.
    ISBN: 9781493988792
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Call number: QZ365WI810:29
    Keywords: Pancreatic Neoplasms / genetics ; Pancreatic Neoplasms / therapy ; Genetic Techniques
    Pages: xi, 352 p. : ill.
    ISBN: 1588291073
    Signatur Availability
    QZ365WI810:29 available
    BibTip Others were also interested in ...