Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1573-7276
    Keywords: calf pulmonary endothelial cells ; human renal cell carcinoma ; invasion and metastasis ; in vitro invasion model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: We developed a modifiedin vitro invasion assay system using monolayers of vascular endothelial cells. A type I collagen gel was formed in plastic dishes, and overlaid with type IV collagen. Calf pulmonary arterial endothelial (CPAE) cells were seeded onto these plates, and incubated until they reached confluence. Five human renal cell carcinoma cell lines with various metastatic potentialsin vivo were then seeded on the monolayer CPAE cells, and their colony formation and invasion activities were examined for 9 days. At day 4, the highly metastatic cell lines increased the number of colony foci on monolayer CPAE cells several fold higher than their poorly metastatic counterpart. The horizontal spreading patterns were also different between poorly and highly metastatic cell lines. On day 9, the number of carcinoma foci that penetrated the monolayer of CPAE cells and type IV collagen sheets into type I collagen gels in highly metastatic cell lines greatly increased as compared with that of poorly metastatic cell lines. Ourin vitro invasion assay using monolayer CPAE cells would be useful to evaluate protease activities and colony formation during invasion.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1615-2573
    Keywords: Hypertrophic cardiomyopathy ; Minimal vascular resistance ; Human forearm ; Strain gauge plethysmography ; Vasodilator capacity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary It is not known whether hypertrophic cardiomyopathy (HCM) is accompanied by an abnormality in vascular smooth muscles. In this study, we examined the maximal vasodilator capacity of forearm resistance vessels by measuring minimal forearm vascular resistance (min. FVR) during peak reactive hyperemia after 10 min of arterial occlusion in patients with HCM (n=15, 41±4 years old) and age-matched control subjects (n=12, 42±3 years old). Forearm blood flow (FBF) was measured by a mercury-in-silastic strain gauge plethysmograph and FVR was calculated by dividing mean blood pressure by FBF. Resting FBF was lower (P〈0.05) and resting FVR was higher (P〈0.01) in patients with HCM than in control subjects. Min. FVR was significantly greater in patients with HCM than in control subjects (2.7±0.2 vs 1.5±0.2 units,P〈0.005). We also examined vasoconstrictive responses to intra-arterially infused angiotensin II (20 and 40 ng/min); responses were greater in patients with HCM than in control subjects (P〈0.05). These results indicate that forearm circulation is altered in patients with HCM. The result that the maximal vasodilator capacity of forearm resistance vessels is limited in patients with HCM as compared with that in age-matched control subjects suggests that there may be abnormalities in forearm resistance vessels in patients with HCM, which might involve increased wall thickness or intrinsic abnormalities in vascular smooth muscle.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract • Background: Although the choroidal neovascularization (CNV) is a common pathologic feature of a number of different eye diseases, its pathological mechanisms have not been fully elucidated. We investigated the expression of vascular endothelial growth factor (VEGF) in CNV using an experimental primate model. • Method: CNV was induced by intense laser photocoagulation in four monkey eyes. Single eyes were enucleated at 1, 3, 7 or 14 days after photocoagulation and examined immunohistochemically for VEGF, macrophage antigen, von Willebrand factor and glial fibrillary acidic protein (GFAP). Expression of VEGF mRNA was examined byin situ hybridization. • Results: One day after photocoagulation, the normal structure of the outer portion of the retina and the inner portion of the choroid was destroyed. Three days after photocoagulation, choroidal vascular endothelial cells migrated into the subretinal space through the defect in Bruch's membrane. Increased expression of VEGF was detected in the accumulating macrophages, migrating retinal pigment epithelial (RPE) cells and Muller cells. Maximal expression of VEGF was observed between 3 and 7 days after wounding, and many newly formed vessels extended into the subretinal space 7–14 days after photocoagulation. • Conclusion: VEGF derived from RPE cells, macrophages and Müller cells may play a role in the formation of CNV.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Monoclonal antibodies (mAbs) were developed against corneal endothelial cells (CECs) using cultured bovine CECs as immunogens by the mouse hybridoma technique. One of these mAbs, KP14D10 (IgM type), reacted specifically with CECs in human, bovine and rabbit ocular tissues as judged by immunohistochemical methods. This mAb also reacted with some epithelial cells of human esophageal and gastric glands but not with those of bovine and rabbit origin. The molecular weight of the antigen was determined to be 60000 Da by the immunoblotting method. This antigenicity was apparently decreased by treatment with hyaluronidase, trypsin, and pronase. In the immunohistochemical study of rabbit fetal corneas, the immunofluorescence appeared specifically in the CECs after the 15th day of gestation and its intensity became stronger as gestation advanced. These results suggest that this antibody would be a valuable tool in the further analysis of corneal development and of divese pathological alterations of CECs.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1432-2307
    Keywords: Bronchiolo-alveolar carcinoma ; Intranuclear inclusion ; Surfactant-apoprotein ; Type II pneumocyte ; Immunoperoxidase method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fifty-five cases of bronchiolo-alveolar carcinoma were examined immunohistochemically using mono-specific antisurfactant apoprotein IgG obtained from a rabbit immunized with monkey surfactant preparations. Formalin-fixed and paraffin-embedded lung tissues were stained by an immunoperoxidase method. Antibody stained the normal and hyperplastic alveolar type II pneumocytes, but did not stain bronchial epithelium or other lung cells. In tumor tissue, 26 (47.3%) of 55 cases were positively stained in the cytoplasm, and 15 showed reaction products in both the cytoplasm and intranuclear regions. By electron microscopy osmiophilic lamellar bodies and microvilli on the free surface, thought to be characteristic of type II pneumocytes, were seen in tumor cells (2 cases). In the five cases, the nuclei contained branching tubular inclusions. The results of this study support the idea that certain bronchiolo-alveolar carcinomas originate from type II pneumocytes, the intranuclear inclusions may represent an abnormal proliferation of nuclear membranes containing surfactant-apoprotein.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1432-2307
    Keywords: Hyaline membrane disease ; Fibrinolysis ; Newborn rabbits ; Antiplasmin ; Hypoxia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The role of fibrinolysis in the pathogenesis of hyaline membrane disease was studied in newborn rabbits at 28- or 29-day gestation. Hyaline membrane was recognized in the lungs of rabbits that were exposed to intrauterine hypoxia and received trans-4-aminomethylcyclohexane carboxylic acid (t-AMCHA), a plasmin inhibitor found to cause a marked decrease in the fibrinolytic activity of lung extracts. In the newborn rabbits subjected to intrauterine hypoxia alone, surface activity of lung extracts was reduced, but hyaline membrane was not seen. By immunofluorescent examination the hyaline membrane was found to be rich in fibrinogen and its derivatives. Electron microscopic examination revealed that they contained various amounts of cellular debris, bundles of fibrils and electron-dense, finely granular deposits and polymerized fibrin with 230 å periodicity. Disintegration of pulmonary epithelial cells was also seen. These findings indicate that diminished fibrinolytic activity of lung tissue contributes to intra-alveolar accumulation of fibrinogen, fibrin and their degradation products, thereby bringing about morphological and physiological disintegration of the terminal airways.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1432-2307
    Keywords: Saphenous vein ; Endothelium ; Smooth muscle cells ; Fibrinogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The distribution of re-endothelialization and the development of intimal thickening were investigated electron microscopically and immunohistochemically using saphenous vein grafts implanted into the femoral artery in dogs. Animals were divided into two groups according to a difference of preparation of the venous grafts before implantation: group 1 without storage, and group 2 with storage for 1 h in 0.9% sodium chloride solution containing papaverine and heparin. In group 2 endothelial cells were almost totally denuded at 1 day, while endothelial islands were always left on the back of valves. In group 1 small islands of surviving endothelial cells were occasionally observed away from the valves. By 1 week, the re-endothelialization of group 1 and group 2 extended to 71 ± 14.7% and 47 ± 9% (mean ± SD) of the total luminal surface area, respectively, and spread mainly from the valves but not from the adjacent artery. Immunohistochemical study using anti-dog fibrinogen IgG suggested that increased permeability of the graft wall continued for one month, in spite of the endothelial covering. Intimal thickening was most pronounced at 1 month, especially near the anastomosis, but was less in the middle of the grafts. These results indicate that intimal thickening of the venous grafts is a response to injury which intimately depends on the rate of re-endothelialization.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    ISSN: 1432-2161
    Keywords: Key words Transient osteoporosis of the hip ; Osteonecrosis ; Osteopenia ; Bone marrow edema ; Femoral head
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Objective. It has been proposed that transient osteoporosis of the hip (TOH) may represent the early reversible phase of osteonecrosis of the femoral head (ON). The purpose of this study was to investigate the clinicopathologic characteristics of three cases of TOH. Design and patients. A bone biopsy was performed on three patients who had been diagnosed as having TOH based on the clinical course, radiograph, bone scintigram, and MR images. The biopsy specimens were studied histopathologically by light and electron microscopy. Results. The most characteristic feature of TOH was focal areas of thin and disconnected bone trabeculae covered by osteoid seams and active osteoblasts. The surrounding bone marrow tissue showed edematous changes and mild fibrosis, frequently associated with vascular congestion and/or interstitial hemorrhage. No osteonecrotic region was observed in either the bone trabeculae or the bone marrow tissue. All patients have improved clinically and in the 3.5–9 years of follow-up have shown no evidence of ON. Conclusions. This study supports the concept that transient osteoporosis of the hip is a distinct entity.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 362 (1974), S. 223-229 
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case of newborn infant, born at 28 weeks gestational age, was incidentally diagnosed as bronchiolitis obliterans at autopsy by being recognized that the widespread bronchiolar stenosis by polypoid masses composed of cicatrical tissue, which was also proved by electron microscopic studies. Though bronchiolitis obliterans was observed in newborns, infants and children as well as in adults, the similar case of intrauterine bronchiolitis obliterans was reported only by Sir in 1962, as bronchiolitis obliterans connata. Because of nature and maturity of the lesion, it was considered that our case was effected by some causative agent two months or more prior to his birth.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    ISSN: 1432-2307
    Keywords: Plasminogen activator ; Fibrinolysis ; Cultured human cancer cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Immunological similarities or differences between urokinase and plasminogen activators from 9 lines of cultured human caner cells with varying degrees of fibrinolytic activity were examined with antibodies against human urokinase. The antibodies completely inhibited the fibrinolytic activity of 4 lines of gastric cancer, 2 lines of lung cancer, 1 line of urinary bladder cancer and 1 line of renal cancer, indicating that the plasminogen activators from these cell lines were immunologically identical to urokinase. In 5 out of these cell lines, immunological identity was also confirmed by double diffusion analysis. The plasminogen activator from 1 line of lung cancer was found to be immunologically dissimilar to urokinase by a neutralization experiment and double diffusion analysis. These findings indicate that there are at least two immunologically distinguishable forms of plasminogen activators from human cancer cells.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...