Blackwell Publishing Journal Backfiles 1879-2005
Bullous pemphigoid (BP) is characterized by the production of autoantibodies against BP antigens, γ-interferon (γ-IFN), a T-cell lymphokine, is known to enhance the expression of several cell-surface proteins. In this study, keratinocytes were cultured in the presence of γ-IFN. the expression of BP antigen protein was examined by flow cytometry and BP antigen messenger RNA (mRNA) (encoding 230-kDa protein) was quantified by slot-blot hybridization. The results indicated that BP antigen gene expression by keratinocytes was upregulated by γ-IFN. This enhancement of gene expression was I detected at both the protein and mRNA level, suggesting pretranslational regulation. These results imply the involvement of not only humoral immunity but also cell-mediated immunity in the development of BP.
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