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  • 1
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    Totowa, N.J. : Humana Press
    Associated volumes
    Call number: A110:5
    Keywords: Epidermis / Physiology ; Epidermis / Cytology ; Epidermis / cytology ; Cell culture / Methods ; Cytological Techniques / methods ; Genetic Engineering / methods ; Keratinocytes / cytology ; Organ Culture / methods
    Description / Table of Contents: Table of contents: Primary mouse keratinocyte culture / Annalisa Pirrone, Barbara Hager, and Philip Fleckman -- Serial cultivation of primary adult murine keratinocytes / Richard P. Redvers and Pritinder Kaur -- Keratinocyte culture in the absence of substrate attachment / Monika Jost and Ulrich Rodeck -- Application of genetically modified feeder cells for culture of keratinocytes / Takashi Kameda and Toshihiro Sugiyama -- Organ culture of developing mouse skin and its application for molecular mechanistic studies of morphogenesis / Mariko Kashiwaga and Nam-ho Huh -- Experimental models to analyze differentiation functions of cultured keratinocytes in vitro and in vivo / Nicole Maas-Szabowski, Norbert E. Fusenig, and Hans-Jürgen Stark -- In vitro fabrication of engineered human skin / Alexander Margulis, Weitian Zhang and Jonathan A. Garlick -- In vivo labeling and analysis of epidermal stem cells / Wei-yang Wu and Rebecca J. Morris -- Method for the harvest and assay of in vitro clonogenic keratinocytes stem cells from mice / Wei-yang Wu and Rebecca J. Morris -- FACS enrichment of human keratinocyte stem cells / Amy Li and Pritinder Kaur -- Isolation, characterization, and culture of epithelial stem cells / Jackie R. Bickenbach -- Keratin 19 as a stem cell marker in vivo and in vitro / Danielle Larouche [et al.] -- Immunolocalization in the epidermis / Tammy-Claire Troy [et al.] -- Epidermal cell analysis by RT-PCR / Tammy-Claire Troy [et al.] -- Whole-mount assays for gene induction and barrier formation in the developing epidermis / Carolyn Byrne and Matthew J. Hardman -- Analysis of early epidermal development in zebrafish / Ashley E. Webb and David Kimelman -- Analysis of E2F factors during epidermal differentiation / Wing Y. Chang and Lina Dagnino -- Analysis of HOX homeodomain proteins and gene transcripts --
    Pages: xv, 480 p. : ill.
    ISBN: 1588292673
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    A110:5 departmental collection or stack – please contact the library
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  • 2
    Keywords: Life sciences ; Stem Cells ; Regenerative Medicine ; Tissue Engineering ; Life sciences ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Springer eBooks
    Description / Table of Contents: 1 The hair follicle stem cell niche: the bulge and its environment -- 2 The sebaceous gland stem cell niche -- 3 Inner ear stem cell niche -- 4 Corneal Limbal Stem Cell Niche -- 5 Liver Stem Cell Niche -- 6 The elusive pancreatic stem cell -- 7 The Intestinal Stem Cell Niche -- 8 Dental Pulp Stem Cell Niche -- 9℗ Vascular Niche in HSC development, maintenance and regulation -- 10 Mesenchymal Stem Cell Niche of the Bone Marrow -- 11 Tendon stem cell niche -- 12 The Male Stem Cell Niche: insights from Drosophila and mammalian model systems.℗
    Abstract: This timely volume explores tissue-derived stem cells, summarizing and evaluating the current body of knowledge. Sixteen chapters cover hematopoietic, osteoblast, skeletal, cardiac, pancreatic, epithelial, corneal, hepatic, renal, testicular, ovarian, fingernail, hair follicle and other stem cells- discussing their individual development, functions, and pathologies, as well as the commonalities and interdependence between niches. This installment of the popular Stem Cell Biology and Regenerative Medicine series delivers authoritative, international perspectives on this rapidly growing field. Tissue-Specific Stem Cell Niche℗ is an ideal complementary volume to℗ Biology of℗ Stem Cell Niche℗ and℗ Adult Stem Cells, Second Edition℗ and will be invaluable to clinicians and researchers working with stem cells as well as to postgraduate trainees who are studying them
    Pages: XII, 328 p. 48 illus., 44 illus. in color. : online resource.
    Edition: 1st ed. 2015.
    ISBN: 9783319217055
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  • 3
    Unknown
    Cham : Springer International Publishing
    Keywords: Life sciences ; Stem Cells ; Biomedical Engineering ; Life sciences ; Stem Cells ; Biomedical Engineering ; Springer eBooks
    Abstract: This comprehensive volume explores functions, pathologies, and applications of stem cells in relation to the niches in which they develop. Ten chapters cover the subject in depth, from a historical perspective through signaling, hormonal control, quiescence, biomimetics, epigenetics, engineering strategies for emulating, tumorigenesis and more. The chapter authors represent a broad range of international expertise and perspectives. This installment of the popular Stem Cell Biology and Regenerative Medicine series delivers authoritative, international perspectives on this rapidly growing field. Biology of Stem Cell Niche is an ideal complementary volume to Tissue-Specific Stem Cell Niche and Adult Stem Cells, Second Edition and will be invaluable to clinicians and researchers working with stem cells as well as to postgraduate trainees who are studying them
    Pages: XII, 228 p. 25 illus., 21 illus. in color. : online resource.
    Edition: 1st ed. 2015.
    ISBN: 9783319217024
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  • 4
    Keywords: Life sciences ; Stem Cells ; Regenerative Medicine ; Tissue Engineering ; Biomedical Engineering ; Life sciences ; Regenerative Medicine/Tissue Engineering ; Stem Cells ; Biomedical Engineering ; Springer eBooks
    Abstract: This℗ volume explores laser-assisted bioprinting, focusing on the most recent developments in its use for℗ tissue engineering. Bringing together authoritative and international perspectives, the text begins with an overview and goes on to cover bioprinting℗ in cell viability and pattern viability, tissue microfabrication to study cell proliferation, microenvironment for controlling stem cell fate, cell differentiation, zigzag cellular tubes, cartilage tissue engineering, osteogenesis, vessel substitutes, skin tissue and much more. Bioprinting is on its way to℗ becoming a℗ dominant technology in tissue-engineering; Bioprinting in Regenerative Medicine, from the bestselling Stem Cell Biology in Regenerative Medicine series, is℗ essential reading for those researching or working in regenerative medicine, tissue engineering,℗ or translational research. Those studying or working with stem cells who are interested in the development of the field will also find the information invaluable
    Pages: XI, 140 p. 31 illus., 28 illus. in color. : online resource.
    Edition: 1st ed. 2015.
    ISBN: 9783319213866
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  • 5
    Keywords: Life sciences ; Biotechnology ; Cytology ; Stem Cells ; Life sciences ; Stem Cells ; Biotechnology ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Signaling Pathways Maintaining Stemness in Adult Hematopoietic Stem Cells -- The Adult Stem Cell Niche -- Adult Stem Cells: Adult Skeletal Muscle Stem Cells -- Adult Cardiac Stem Cells: Identity, Location and Potential -- Adult Pituitary Stem Cells -- Toward Translating Molecular Ear Development to Generate Hair Cells from Stem Cells -- Adult Human Corneal Epithelial Stem Cells -- Adult Stem Cells in Teeth -- Adult Mammary Stem Cells: Identity, Location and Functional Assays -- Adult Ovary Stem Cells -- Adult Prostate Stem Cells -- Adult Lung Stem Cells -- Adult Liver Stem Cells -- Lineage-committed Pancreatic Progenitors and Stem Cells -- Adult Stromal (Skeletal, Mesenchymal) Stem Cells -- Regeneration after Injury - Activation of Stem Cell Stress Response Pathways to Rapidly Repair Tissues -- Molecular and Endocrine Mechanisms Underlying the Stem Cell Theory of Aging
    Abstract: Adult Stem Cells, second edition, takes a critical look at issues concerning the℗ developmental or differentiation potential for a variety of tissue types and for specific adult stem cell types. Since the first edition appeared a decade ago, our understanding of adult stem cells, and more specifically tissue-specific adult stem cells, has advanced tremendously. And an increased interest in regenerative medicine and potential stem cell applications℗ has driven a quest for better understanding of stem cell biology. In turn, this has spawned much activity on generation and utilization of more and better reagents to identify and isolate stem cells and stem cell-like subpopulations, and on assays elucidating their developmental or differentiation potential and functional integration with host tissues and organs. In this fully updated new edition, chapters cover topics ranging from signaling pathways maintaining stemness in hematopoietic cells to regeneration after injury and endocrine mechanisms underlying the stem cell theory of aging. Other chapters cover℗ stem cells by organ or system including pituitary, cardiac, epithelial, teeth, lung, ovary, prostate, liver, and many more.℗ Importantly, the authors of the chapters have not only summarized their successes, but have also summarized some of the difficulties that each particular field is still facing with respect to maximizing the utility of stem cells in clinical settings. Collectively, they impart both the excitement and challenges facing stem cell utilization for repair and regeneration℗ making this book essential reading for those involved in stem cell research as well as those involved in clinical assays
    Pages: XIII, 429 p. 29 illus., 28 illus. in color. : online resource.
    Edition: 2nd ed. 2014.
    ISBN: 9781461495697
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  • 6
    Keywords: Life sciences ; Cytology ; Stem Cells ; Cell Culture ; Life sciences ; Cell Biology ; Stem Cells ; Cell Culture ; Springer eBooks
    Description / Table of Contents: Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage -- Differentiation of Epidermal Keratinocytes from Human Embryonic Stem Cells -- Protocol for Serial Cultivation of Epithelial Cells Without Enzymes or Chemical Compounds -- Growth and Differentiation of HaCaT Keratinocytes -- Transgene Delivery to Cultured Keratinocytes Via Replication-Deficient Adenovirus Vectors -- Analyzing the Global Chromatin Structure of Keratinocytes by MNase-Seq -- Analysis and Meta-Analysis of Transcriptional Profiling in Human Epidermis -- Compound Screening and Transcriptional Profiling in Human Primary Keratinocytes: A Brief Guideline -- Preparation of Primary Cultures of Mouse Epidermal Keratinocytes and the Measurement of Phospholipase D Activity -- Lipid Rafts and Detergent-Resistant Membranes in Epithelial Keratinocytes -- MMP-2, 9 and TIMP-1, 2 Assays in Keratinocyte Cultures -- Reactive Oxygen Species (ROS) Protection Via Cysteine Oxidation in the Epidermal Cornified Cell Envelope -- Modified Methods for Growing 3-D Skin Equivalents: An Update -- A Novel Three-Dimensional Cell Culture Method to Analyze Epidermal Cell Differentiation In Vitro -- Reconstruction of Normal and Pathological Human Epidermis on Polycarbonate Filter -- Methods for the Preparation of an Autologous Serum-Free Cultured Epidermis and for Autografting Applications -- Human Keratinocyte Cultures in the Investigation of Early Steps of Human Papillomavirus Infection -- Preparation and Delivery of 4-Hydroxy-Tamoxifen for Clonal and Polyclonal Labeling of Cells of the Surface Ectoderm, Skin, and Hair Follicle -- Microdissection and Visualization of Individual Hair Follicles for Lineage Tracing Studies -- Isolation and Characterization of a Stem Cell Side-Population from Mouse Hair Follicles -- Multi-Scale Mathematical Modeling and Simulation of Cellular Dynamical Process
    Abstract: Reflecting over three decades of advances, Epidermal Cells: Methods and Protocols, Third Edition underscores these advances in our understanding of epidermal biology with updated and entirely new protocols that compliment and extend the earlier edition. The inclusion of protocols useful for both in vitro and in vivo studies reflects many useful developments in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Dependable and easy to follow, Epidermal Cells: Methods and Protocols, Third Edition serves researchers working to accelerate the work in this vital field of study
    Pages: XI, 296 p. 150 illus., 34 illus. in color. : online resource.
    Edition: 3rd ed. 2014.
    ISBN: 9781493912247
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  • 7
    Keywords: Life sciences ; Cell Biology ; Life sciences ; Cell Biology ; Springer eBooks
    Description / Table of Contents: Measuring Sphingosine-1-Phosphate/Protein Interactions with the Kinetic Exclusion Assay -- An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity -- Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells -- Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry -- Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines -- A Clean-Up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule -- A Rapid Fluorescence Assay for Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes -- Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-Parametric Flow Cytometry -- S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses -- In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate -- Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor -- Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors -- Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation -- 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering
    Abstract: This fully updated edition explores methods involving sphingosine-1-phosphate (S1P), a bioactive lysophospholipid which has become the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells. Here, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. As a volume in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols, Second Edition is another key resource for scientists working in this fast-moving and dynamic field
    Pages: X, 182 p. 26 illus. : online resource.
    Edition: 2nd ed. 2018.
    ISBN: 9781493974139
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  • 8
    Keywords: Life sciences ; Cytology ; Stem Cells ; Life sciences ; Cell Biology ; Stem Cells ; Springer eBooks
    Description / Table of Contents: Therapeutic Mesenchymal Stromal Cells: Where We Are Headed -- The Suspension Culture of Undifferentiated Human Pluripotent Stem Cells Using Spinner Flasks -- Scalable Expansion of Human Induced Pluripotent Stem Cells in Xeno-Free Microcarriers -- Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs -- Labeling Pluripotent Stem Cell-Derived Neural Progenitors with Iron Oxide Particles for Magnetic Resonance Imaging -- Using the Quantum Cell Expansion System for the Automated Expansion of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells -- Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions -- Production of Good Manufacturing Practice-Grade Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells for Therapeutic Use -- Xeno-Free Culture of Human Periodontal Ligament Stem Cells -- GMP-Compliant Human Adipose Tissue-Derived Stromal Cells for Cellular Therapy -- cGMP-Compliant Transportation Conditions for a Prompt Therapeutic Use of Marrow Mesenchymal Stromal/Stem Cells -- GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation -- Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting -- Scalable Ex Vivo Expansion of Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Stirred Culture Systems -- Media Fill for Validation of a Good Manufacturing Practice-Compliant Cell Production Process -- Standard Operating Procedure for the Good Manufacturing Practice-Compliant Production of Human Bone Marrow Mesenchymal Stem Cells -- Culture of Human Limbal Epithelial Stem Cells on Tenon’s Fibroblast Feeder-Layers: A Translational Approach -- Bioreactor Expansion of Human Mesenchymal Stem Cells According to GMP Requirements
    Abstract: This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies. Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations pulls together key GMP techniques from laboratories around the world. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Inclusive and authoritative, Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations will be an invaluable resource to both basic and clinical practitioners in stem cell biology
    Pages: XI, 220 p. 34 illus. in color. : online resource.
    ISBN: 9781493924356
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  • 9
    Keywords: Life sciences ; Cytology ; Stem Cells ; Life sciences ; Cell Biology ; Stem Cells ; Springer eBooks
    Description / Table of Contents: Propagation and Differentiation of Human Whartoń€™s Jelly Stem Cells on Three Dimensional Nanofibrous Scaffolds -- Composite Electrospun Nanofibers for Influencing Stem Cell Fate -- In Vitro Nanoparticle-Mediated Intracellular Delivery into Human Adipose-Derived Stem Cells -- Screening of Nanoparticle Embryotoxicity Using Embryonic Stem Cells -- Peptide Nanofiber Scaffolds for Multipotent Stromal Cell Culturing -- Patterned Polymeric Surfaces to Study the Influence of Nanotopography on the Growth and Differentiation of℗ Mesenchymal Stem Cells -- Transduction of Murine Embryonic Stem Cells by Magnetic Nanoparticle-Assisted Lentiviral Gene Transfer -- Lanthanide-Based Upconversion Nanoparticles for Connexin-Targeted Imaging in Co-Cultures -- Basic Protocols to Investigate hMSC Behavior onto Electrospun Fibers -- General Protocol for the Culture of Cells on Plasma-Coated Electrospun Scaffolds -- Mesenchymal Stem Cells and Nano-Structured Surfaces -- Chondrogenic Differentiation of Menstrual Blood-Derived Stem Cells on Nanofibrous Scaffolds -- Engineering of a 3D Nanostructured Scaffold Made of Functionalized Self-Assembling Peptides and Encapsulated Neural Stem Cells
    Abstract: Stem Cell Nanotechnology: Methods and Protocols gathers several representative protocols related to the emerging interest in nanotechnology as it relates to stem cell biology. The detailed chapters presented within have been validated for reproducibility and are described in an easy to follow, step-by-step fashion so as to be valuable for not only experts but also novices in the stem cell field.℗ As with other volumes in the highly successful Methods in Molecular Biology series, chapters conclude with a Notes section, which provides tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and practical, Stem Cell Nanotechnology: Methods and Protocols provides both a flavor of the field as it currently is and a source to stimulate new approaches and methodologies by those interested in nanotechnological applications
    Pages: XI, 184 p. 50 illus., 11 illus. in color. : online resource.
    ISBN: 9781627035712
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  • 10
    Keywords: Life sciences ; Oncology ; Reproductive Medicine ; Stem Cells ; Life sciences ; Stem Cells ; Cancer Research ; Reproductive Medicine ; Springer eBooks
    Description / Table of Contents: Myogenic Potential of Stem Cells: In Vivo Assessment -- Urine DerivedSstem Cells: Biological Characterization and Potential Clinical Applications -- Immunoregulatory Properties of Mesenchymal Stem Cells: In Vitro and in Vivo -- Current Research on Stem Cells in Parkinsoń€™s Disease: Progress and Challenges -- Stem Cell Applications in Retinal Diseases -- Potential Use of Dental Stem Cells for Craniofacial Tissue Regeneration -- Pediatric Diseases and Stem Cells:℗ Recent Advances and Challenges -- Stem Cell Therapy ́€“ From the Heart to the Periphery -- Stem Cells in Wound Healing -- Cancer Stem Cells: The Gist of the Matter -- Filling the Gap in the Relationship Between Cancer and Stem Cells -- Therapy Resistance in Prostate Cancer: A Stem Cell Perspective -- Mechanisms of Somatic Cell Reprogramming -- Disease Modeling and Drug Discovery Using Human Pluripotent Stem Cells -- Stem Cell Research: Ethical Considerations
    Abstract: This latest volume in Springer's℗ Stem Cell Biology and Regenerative Medicine series℗ looks at the state-of-the-science in stem cells, discusses the℗ current challenges, and examines the℗ new directions the field is taking. Dr. Turksen,℗ Editor-in-Chief of the journal℗ Stem Cell Reviews and Reports, has℗ assembled℗ a volume℗ of internationally-known scientists who cover topics that℗ are℗ both research and clinically oriented. The contents range from sources of stem cells through℗ their physiological role in℗ health and disease, therapeutic applications in regenerative medicine and ethics and society. An initial overview and a final summary bookend the contents into a cohesive and invaluable volume
    Pages: IX, 373 p. 23 illus., 21 illus. in color. : online resource.
    ISBN: 9781461480662
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