Plant Genetics and Genomics
Description / Table of Contents:
A Low-Cost High-throughput Method for Plant Genomic DNA Isolation -- Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement -- Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping -- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops -- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing -- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR -- DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics -- Genome-wide Identification of Regulatory DNA Elements in Crop Plants -- Genome-wide Profiling of Histone Modifications with ChIP-seq -- Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes -- Genome-wide Identification of Allele-Specific Gene Expression in a Parent€‘óf€‘Órigin Specific Manner -- Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP) -- Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis -- Emerging Genome Engineering Tools in Crop Research and Breeding -- CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids -- Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne -- Agrobacterium Transformation in the Rice Genome -- In vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C4 Photosynthesis in Zea mays
The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field
XI, 247 p. 30 illus., 21 illus. in color. : online resource.
1st ed. 2020.