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  • 1
    Keywords: Life sciences ; Proteins ; Systems Biology ; Life sciences ; Protein Science ; Protein Structure ; Systems Biology ; Springer eBooks
    Description / Table of Contents: Protein complex production from the drug discovery standpoint -- Choose a suitable expression host: a survey of available protein production platforms -- ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts -- Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput -- Membrane protein production in E. coli for applications in drug discovery -- Cell-free Synthesis of Macromolecular Complexes -- A Bacillus megaterium system for the production of recombinant proteins and protein complexes -- Protein complex production in alternative prokaryotic hosts -- Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts -- Leishmania tarentolae for the Production of Multi-Subunit Complexes -- Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes -- Fundamentals of baculovirus expression and applications -- The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics -- Fundamentals of expression in mammalian cells -- Assembling multi-subunit complexes using mammalian expression -- Microalgae as solar-powered protein factories -- Strategies and methodologies for the co-expression of multiple proteins in plants -- Transient expression systems in plants—potentialities and constraints -- Complex reconstitution from individual protein modules -- Structural reconstruction of protein-protein complexes involved in intracellular signaling -- The use of small-angle scattering for the characterization of multi subunit complexes -- Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems
    Abstract: This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance. This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies
    Pages: XIII, 382 p. 87 illus., 71 illus. in color. : online resource.
    ISBN: 9783319272160
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  • 2
    Keywords: Medicine ; Toxicology ; Pharmaceutical technology ; Life sciences ; Biochemistry ; Animal Physiology ; Biomedicine ; Biomedicine general ; Pharmacology/Toxicology ; Pharmaceutical Sciences/Technology ; Life Sciences, general ; Animal Biochemistry ; Animal Physiology ; Springer eBooks
    Abstract: Scorpions have fascinated humans for a long time, Ơ̐rst and foremost because of the harm the sting of a few species could cause but also due to their unique natural history and for the many biologically active compounds found in their venoms. This volume of the Toxinology handbook series covers all those aspects. The subjects are divided into seven sections starting with an introduction to the general aspects of scorpion biology and ecology, followed by the description of the €œénvenomatioń€ pathophysiology, pharmacokinetics, and pharmacodynamics of venoms and their complex interactions with the immune system. The future of anti-scorpion venom therapy is then covered in two chapters dedicated to alternatives to the century-old techniques currently used to produce €œánti-venomś€. The next section presents a world tour of ́€œscorpionisḿ€ and dangerous scorpion species and their impact on human health. It is worth remembering that envenomation due to scorpion stings is a substantial health hazard in Asian, Middle Eastern, African, and Latin American countries, with over one million people stung by scorpions every year, resulting in more than 3,000 deaths. Species-centered overviews of ́€œscorpion venomś€ are presented in the next section, after which a section details the two main types of ́€œscorpion toxinś€. The last section covers high-throughput transcriptome and proteome screenings now known as ́€œvenomicś€. ℗ This volume provides chapters that are accessible to a broad audience, yet also contains the depth of knowledge and detail which makes it of interest to experts in the field. Readers of this reference work will leave well informed on the current state of the art of scorpion venom research
    Pages: XXV, 575 p. 115 illus., 92 illus. in color. eReference. : online resource.
    ISBN: 9789400764040
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  • 3
    Keywords: Life sciences ; Ecology ; Microbiology ; Plant Diseases ; Entomology ; Life sciences ; Entomology ; Ecology ; Plant Pathology ; Microbiology ; Springer eBooks
    Pages: : digital
    ISBN: 9789048139668
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  • 4
    Keywords: Molecular Biology ; Cancer Research ; Pharmaceutical technology ; Immunology ; Biomedical Engineering ; Genetics ; Molecular Medicine ; Cancer Research ; Pharmaceutical Sciences/Technology ; Immunology ; Biomedical Engineering/Biotechnology ; Genetics and Genomics ; Springer eBooks
    Description / Table of Contents: Preface -- Pharmaco-Geno-Proteo-Metabolomics and Translational Research in Cancer -- Next generation sequencing (NGS): a revolutionary technology in pharmacogenomics and personalized medicine in Cancer -- Pharmaco-epigenomics: on the road of translation medicine -- Design and Implementing Pharmacogenomics Study in Cancer -- Onco-omics approaches and applications in Clinical Trials for Cancer patients -- Issues and Ethical Considerations in Pharmaco-oncogenomics -- Pharma-oncogenomics in the era of personal genomics: a quick guide to online resources and tools -- Inmuno-Oncology in the era of Personalized Medicine -- CAR T Cell and Personalized Medicine -- Oncobiome at the forefront of a novel molecular mechanism to understand the Microbiome and Cancer -- Nutrition, Cancer and Personalized Medicine -- Index
    Abstract: This book presents concise chapters written by internationally respected experts on various important aspects of translational cancer genomics, offering a comprehensive overview of the onco-omics applications in the new era of cancer personal genomics research field. Being a complex disease that affects millions of people worldwide, cancer research has assumed great significance. Translational cancer research transforms scientific discoveries in the laboratory into clinical application to reduce incidence of cancer, morbidity and mortality. In the other hand, personalized medicine in cancer is the concept that selection of a treatment should be tailored according to the individual patient’s specific genomic characteristics, including mutations, chromosomal aberrations, protein interactions, and even more, considering the immune system, the metabolism and in the near future the microbiome, all areas nowadays related in the prognosis and treatment of cancer patients. These contributions are either critical reviews or clinical and basic research reports. The topics discussed here include evidence-based medicine and provides useful information on translational cancer research to clinicians and biomedical scientists in order to offer another vision of cancer origin beyond the traditional causes or the oncogenic maintenance concept proposed by the oncogene addiction theory
    Pages: X, 171 p. 19 illus. in color. : online resource.
    Edition: 1st ed. 2019.
    ISBN: 9783030241001
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  • 5
    ISSN: 1399-3038
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Cutaneous lesions caused by pine processionary caterpillar (Thaumetopoea pityocampa, TP) are frequent in pinewood areas. However, no epidemiological studies have been performed so far in the pediatric population. In this study, we evaluated the relevance of reactions to pine processionary caterpillar in the pediatric population of our environment and determined the possible role of an IgE-mediated mechanism.A questionnaire was developed and given to 1101 children and adolescents ranging from 3 to 17 years of age from rural areas with large pine forests. A total of 653 questionnaires were answered by the children or their parents. The search for immunoglobulin E (IgE)-mediated cases was based on the presence of a suspected reaction to caterpillar and confirmed by in vivo (skin prick) and in vitro (immunoblotting) tests.Sixty of the 653 people interviewed (9.18%) identified a cutaneous reaction from exposure to the processionary caterpillar. Within this group only four cases (6.7%) were because of an IgE-mediated mechanism. The predominant clinical feature of these patients with allergy to caterpillar was contact urticaria.We present the first study of cutaneous reactions to pine processionary caterpillar in a large pediatric population. This is a frequent pathology in pinery zones and only in a minority of the cases are because of an IgE-mediated allergic mechanism. This fact contrasts with studies in adults, where this percentage is much greater.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Munksgaard International Publishers
    Allergy 58 (2003), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-7195
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Resumé Objectif: Déterminer si l'administration d'agonistes de la gonadolibérine (GnRH) provoque des changements dans les paramètres biochimiques, hormonaux et endométriaux chez les femmes qui allaitent. Protocole et population: A partir de la sixième semaine après l'accouchement, des mères allaitant leur nourrisson ont commencé un traitement avec un agoniste de la gonadolibérine, recevant, par pulvérisation nasale, 300 μg et 600 μg par jour pendant 6 mois. Dans un troisième groupe, des nourrices qui portaient des DIU ont servi de témoins. Dix-huit analyses biochimiques ont été effectuées pour mesurer le sérum. Des échantillons sanguins ont été prélevés 0, 3 et 6 mois après le traitement et des biopsies endométriales ont été obtenues à 0 et 6 mois. Aucune autre méthode de contraception n'a été employée. Résultats: On a observé les différences clinico-chimiques entre individus et entre groupes. Selon les niveaux hormonaux et l'analyse histopathologique, divers degrés d'activité folliculaire ont été constatés dans les groupes traités. Aucune grossesse n'est survenue. Conclusion: Le traitement par un agoniste de la gonadolibérine n'ayant eu aucun effet délétère significatif sur les paramètres étudiés, il pourrait représenter une solution possible et sans danger de contraception dès le post-partum.
    Abstract: Resumen Objetivo: Determinar si la administración de un agonista de la GnRH induce cambios en parámetros bioquímicos, hormonales y de endometrio en mujeres que amamantan. Diseño y pacientes: Comenzando a las 6 semanas de posparto, las madres que amamantaban exclusivamente iniciaron el tratamiento con un agonista de la GnRH, recibiendo 300 μg y 600 μg diarios mediante pulverización nasal durante 6 meses. Un tercer grupo do mujeres que amamantaban y eran usuarias de DIU sirvieron de control. Se cuantificaron dieciocho análisis bioquímicos en suero. Se tomaron muestras de sangre a los 0, 3 y 6 meses de tratamiento y se hicieron biopsias de endometrio a los 0 y 6 meses. No se utilizó ningún otro método anticonceptivo. Resultados: Se observaron diferencias entre mujeres y entre grupos en la clínica química. Según los niveles hormonales y el análisis histopatológico, se determinaron diversos grados de actividad folicular en los grupos tratados. No hubo embarazos. Conclusión: Dado que el tratamiento con un agonista de la GnRH no ejerció ningún efecto perjudicial significativo sobre los parámetros estudiados, este tratamiento podría representar un enfoque factible y seguro de la anticoncepción posparto.
    Notes: Abstract Objective: To determine whether GnRH agonist administration induces changes in biochemical, hormonal and endometrial parameters in breastfeeding women. Design and patients: Starting at 6 weeks postpartum, fully breastfeeding mothers began treatment with GnRH agonist, receiving 300 μg and 600 μg per day by nasal spray for 6 months. A third group of breastfeeding women who were users of IUDs served as controls. Eighteen biochemical analyses were quantified in serum. Blood samples were collected at 0, 3 and 6 months after treatment and endometrial biopsies were obtained at 0 and 6 months. No other method of contraception was employed. Results: Interindividual and intergroup differences were observed in clinical chemistry. According to the hormonal levels and the histopathologic analysis, various grades of follicular activity were found in the treated groups. No pregnancies occurred. Conclusion: Because GnRH agonist treatment had no significant deleterious effects on the parameters studied, this treatment could represent a feasible and safe approach to postpartum contraception.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1084
    Keywords: Key words: Carotid transposition ; Spiral CT ; Retropharyngeal mass ; Aberrant internal carotid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. We present four cases of internal carotid artery (ICA) transposition which caused widening of the retropharyngeal soft tissues. Internal carotid artery transposition is a rare cause of retropharyngeal space enlargement, which can be accurately diagnosed in a non-invasive manner with spiral CT. Radiologists should be aware of this entity in the differential diagnosis of retropharyngeal space enlargement in symptomatic (dysphagia, pulsatile mass) and asymptomatic patients, and alert the clinician of its existence, due to the risk of massive bleeding if neck surgery is to be performed.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1600-0536
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Nowadays, caterpillars are included among the agents that elicit contact urticaria by a non-immunologic mechanism. Our objective was to find the rôle that an IgE-mediated mechanism could have among patients with suspected contact urticaria from pine processionary caterpillars. 16 patients with suspected contact urticaria from this caterpillar were studied by prick testing and specific IgE detection by immunoblotting. 87% of the patients had a positive prick test and immunoblotting for caterpillar extract. In these allergic patients, the symptoms associated with urticaria were: angioedema (79%), conjunctivitis (36%) and severe anaphylaxis (14%). The most frequent localizations of the wheals were the neck (100%) and forearms (93%). Angioedema was more frequent on the eyelids (79%). The IgE-immunoblot detected in the caterpillar extract several reactive bands, with apparent MWs from to 45 to 4kDa. A total of 5 major allergens were identified, but a band around 14kDa proved to be the dominant allergen. Sensitization to Thaumetopoea pityocampa was found to be the most important mechanism of airborne contact urticaria from this caterpillar. Low-MW proteins are the main IgE binding components of crude caterpillar extract.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0020-711X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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