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  • 1
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    Champaign, Ill : Project Gutenberg
    Keywords: World War, 1914-1918, Fiction. ; Fantasy fiction., gsafd
    ISBN: 0-585-01435-3
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  • 2
    Keywords: Medicine ; Gastroenterology ; Clinical Medicine ; Transplantation of organs, tissues, etc ; Medicine & Public Health ; Gastroenterology ; Hepatology ; Transplant Surgery ; Springer eBooks
    Pages: : digital
    ISBN: 9781607618669
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  • 3
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    New York, NY : Springer New York
    Keywords: Medicine ; Molecular Biology ; Cardiology ; Animal models in research ; Biomedicine ; Molecular Medicine ; Cardiology ; Animal Models ; Springer eBooks
    Description / Table of Contents: Use of Mouse Models in Atherosclerosis Research -- Cytokines and Immune Responses in Murine Atherosclerosis -- Intravital Microscopy for Atherosclerosis Research -- Non-invasive Molecular Imaging of Mouse Atherosclerosis -- Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root -- Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages -- Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies -- Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay -- Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta -- Immunostaining of Lymphocytes In Mouse Atherosclerotic Plaque -- Flow Cytometric Analysis of Immune Cells Within Murine Aorta -- In Vitro Differentiation of Naïve CD4+ T cells: A Tool for Understanding the Development of Atherosclerosis -- Quantification of Apoptosis in Mouse Atherosclerotic Lesions -- Quantification of Cellular Proliferation in Mouse Atherosclerotic Lesions -- Quantification of in vitro Macrophage Cholesterol Efflux and in vivo Macrophage-Specific Reverse Cholesterol Transport -- In vitro Macrophage Phagocytosis Assay -- Glucose and Insulin Tolerance Tests in the Mouse -- Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries -- Generation of Aorta Transcript Atlases of Wild-type and Apolipoprotein E-null Mice by Laser Capture Microdissection-based mRNA Expression Microarrays -- Analysis of Gene and Protein Expression In Atherosclerotic Mouse Aorta By Western Blot and Quantitative Real-Time PCR -- Bone Marrow Transplantation in Mice to Study the Role of Hematopoietic Cells in Atherosclerosis -- Tandem Stenosis to Induce Atherosclerotic Plaque Instability in the Mouse -- Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions -- Mechanical Stabilization of Mouse Carotid Artery for in vivo intravital Microscopy Imaging of Atherogenesis -- Intravital Microscopy in the Cremaster Muscle Microcirculation for Endothelial Dysfunction Studies. - Fluorescent Molecular Tomography for in vivo Imaging of Mouse Atherosclerosis -- In vivo 18F-FDG-PET Imaging in Mouse Atherosclerosis -- Magnetic Resonance Imaging of the Atherosclerotic Mouse Aorta
    Abstract: This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and valuable notes based on the authors’ personal wet lab experience. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Methods in Mouse Atherosclerosis will be a valuable working guide for researchers performing mouse-based atherosclerosis studies
    Pages: XIV, 400 p. 74 illus., 58 illus. in color. : online resource.
    Edition: 1st ed. 2015.
    ISBN: 9781493929290
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  • 4
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    Madrid : Springer Healthcare
    Keywords: Medicine ; Family medicine ; Public Health ; Practice of medicine ; Medicine & Public Health ; Health Administration ; General Practice / Family Medicine ; Public Health ; Springer eBooks
    Pages: : digital.
    ISBN: 9788493806255
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  • 5
    Keywords: Medicine ; Immunology ; Biomedicine ; Immunology ; Springer eBooks
    Description / Table of Contents: EM Tomography of LINC Complexes -- Recombinant Purification of the Periplasmic Portion of the LINC Complex -- Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis -- Analysis of Nesprin-2 Interaction with its Binding Partners and Actin -- Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID -- Using Nesprin Tension Sensors to Measure Force on the LINC Complex -- Analyzing Mechanotransduction through the LINC Complex in Isolated Nuclei -- Direct Force Probe for Nuclear Mechanics -- Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex Dependent Mechanisms of Homeostatic Nuclear Positioning -- Assembly and use of a Microfluidic Device to Study Cell Migration in Confined Environments -- Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy -- Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super-resolution Imaging -- Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes during Development of Caenorhabditis elegans -- High-resolution Imaging Methods to Analyze LINC Complex Function during Drosophila Muscle Development -- Computational Methods for Studying the Plant Nucleus -- Investigation of Nuclear Periphery Protein Interactions in Plants using the Membrane Yeast Two-Hybrid (MbY2H) System -- Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components -- Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice -- An In Vitro System to Measure the Positioning, Stiffness, and Rupture of the Nucleus in Skeletal Muscle -- Functional Analysis of LINC Complexes in Skin -- Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer -- Next-generation Sequencing and Mutational Analysis: Implications for Genes Encoding LINC Complex Proteins
    Abstract: This book details the most comprehensive, up-to –date, and cutting-edge protocols used in wet and dry labs to investigate the viral communities harbored within and on the human body. Chapters guide readers through methods on collection, isolation, identification and computational/statistical analysis, and body niches to cover those methodological issues inherent to the human tissues and organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Human Virome: Methods and Protocols aims to facilitate researchers with their daily work in the field of the research on the human virome
    Pages: XI, 274 p. 42 illus., 34 illus. in color. : online resource.
    ISBN: 9781493986828
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  • 6
    Keywords: Medicine ; Orthopedics ; Orthopedic surgery ; Physical Therapy ; Sports Medicine ; Medicine & Public Health ; Orthopedics ; Conservative Orthopedics ; Surgical Orthopedics ; Sports Medicine ; Physiotherapy ; Springer eBooks
    Pages: : digital
    ISBN: 9780857295071
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  • 7
    Keywords: Medicine ; Radiology, Medical ; Endocrinology ; Pediatrics ; Medicine & Public Health ; Imaging / Radiology ; Diagnostic Radiology ; Endocrinology ; Pediatrics ; Springer eBooks
    Pages: : digital
    ISBN: 9783642237621
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  • 8
    ISSN: 1600-0757
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objective: The aim of this work was to evaluate the behaviour of B16-F10 melanoma cell cultures implanted in the anterior chamber of the eye of New Zealand white rabbits by studying the clinical-pathological and ultrastructural characteristics of the lesions. Methods: One group (A) (consisting of 30 rabbits) was transclerally inoculated (1 mm from sclero-corneal limbus) with 4×106 melanocytes and another group (B) (also 30 animals) was inoculated once per week for 3 consecutive weeks with 5×106 cells (total 15×106); 30 animals acted as the control group (C). All the lesions were processed for optic and electronic microscopy. Results: Tumoral growth in group A was 43% (13/30) and in group B 80% (24/30). All lesions were pigmented and none perforated the eyeball. Microscopically, they were a mixture of epithelioid and fusiform cells disposed around the blood vessels. Ultrastructurally, the presence of melanosomes in different stages of maturation and aberrant melanosomes were characteristic. Conclusion: We suggest that the transcleral inoculation of 15×106 B16-F10 melanocytes into the anterior chamber of the eye of New Zealand white rabbits may be a valid and reproducible method for obtaining an experimental ocular melanoma model.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1612-1112
    Keywords: Gas chromatography ; Molecular absorption spectrometry ; Gas phase detection ; Benzene derivatives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A simple and inexpensive detection system for gas chromatography, based on gas-phase, molecular absorption measurements, is presented in which the chromatographic column is directly joined to the spectrophotometer flow cell, without heated transfer lines. A mixture of eight benzene compounds (including methyl, halogen and nitrogen derivatives) were separated and analyzed. Parameters affecting separation (temperature program and carrier gas flow) and measurement quality (wavelength and integration time) were studied and a measurement program designed to modify the wavelength during chromatography. The analytical characteristics of each compound were calculated, obtaining detection limits ranging from 0.5 to 9 μg mL−1. Finally, the method was applied to several synthetic mixtures, with good results.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1520-5002
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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