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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 282 (1955), S. 464-466 
    ISSN: 1435-2451
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Mechanisms of cell deposition from a sessile liquid phase and adherence to various plastic substrates have been investigated by measurement of short-term (〈120 min) cell-attachment rates. Sigmoidal attachment-rate curves were fit with a three-parameter variant of a logistic equation to quantify parameters related to initial rate and equilibrium-adherence. For substrates on which cell adhesion was low, initial rates were estimated from slopes of linear bestfit equations. Average variations in adherence parameters for three cell lines, MDCK (epithelioid), VERO, and AHL-1 (fibroblastic) to tissue-culture grade polystyrene dishes were less than 10% (standard-error-of-mean/mean × 100) over extended periods of more than two months, so that attachment measurements could be repetitively applied with the same cell stocks continuously subcultured in the laboratory. Developed techniques were applied to a variety of plastics and the results tabulated. Comparison of adherence parameters for ionomer and polyethylene films, both with and without adsorbed fetal-bovineserum proteins, demonstrated that surface carboxyl groups were important in protein adsorption and cellular adherence. Attachment rates of MDCK to polystyrene were dependent on starting cell number whereas equilibrium adherence did not vary significantly over a wide range of inoculum concentrations. It was concluded from theoretical considerations that initial rates of MDCK attachment were sensitive to and dependent on electrostatic barriers to formation of close substrate contacts whereas equilibrium-adherence levels were controlled by short-range forces such as interfacial energies and formation of receptor-ligand complexes.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Model biomaterial surfaces with well defined chemistry were prepared from close-packed alkyltrichlorosilane monolayers on polished silicon and glass. The outermost molecular groups which come in direct contact with the biological environment were varied across a wide range of oxidation states by employing —CF3, —CH3, —CO2CH3, and —CH2OH terminal functionalities. Characterization by contact angles, surface spectroscopy, and ellipsometry verified that these model surfaces could be repeatedly prepared with good consistency for routine use to study biomolecule adsorption onto model surfaces. Adhesion of canine endothelial cells and the adsorption of proteins (human serum albumin and human fibrinogen) as well as a series of synthetic defined oligopeptides to these model surfaces have been studied. Endothelial cells attachment and growth were in the rank order of: —CH2OH 〉 —CO2Me 〉 —CH3 〉 —CF3. The peptides were comprised of different alternating sequences of lysine, leucine, and tryptophan residues. These structural differences imparted different amphiphilic characters that led to measurable differences in the adsorption of these peptides to liquid-vapor interfaces. The adsorption to model surfaces was studied using ESCA, radiometry, and concentration-dependent contact angles. ESCA and radiometry measured irreversible biomolecules adsorption whereas the contact angle method measured steadystate adsorption. Radiometric results were inconsistent with ESCA, possibly due to artifacts associated with protein radiolabeling. © 1993 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 100 (1978), S. 3163-3166 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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