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  • 1
    Keywords: Life sciences ; Cytology ; Stem Cells ; Life sciences ; Cell Biology ; Stem Cells ; Regenerative Medicine/Tissue Engineering ; Springer eBooks
    Description / Table of Contents: Derivation and Network Formation of Vascular Cells from Human Pluripotent Stem Cells -- High-Throughput Cell Aggregate Culture for Stem Cell Chondrogenesis -- Microfluidic Device to Culture 3D In Vitro Human Capillary Networks -- Multi-Function Co-Culture Model for Evaluating Cell-Cell Interactions -- Multiwell Plate Tools for Controlling Cellular Alignment with Grooved Topography -- Bioreactor Cultivation of Anatomically-Shaped Human Bone Grafts -- Determining the Role of Matrix Compliance in the Differentiation of Mammary Stem Cells -- Conjugation of Proteins to Polymer Chains to Create Multivalent Molecules -- An Assay to Quantify Chemotactic Properties of Degradation Products from Extracellular Matrix -- Biomimetic Strategies Incorporating Enzymes into CaP Coatings Mimicking the In Vivo Environment -- Fabrication of Biofunctionalized, Cell-Laden Macroporous 3D PEG Hydrogels as Bone Marrow Analogs for the Cultivation of Human Hematopoietic Stem and Progenitor Cells -- Extracellular Matrix Mimetic Peptide Scaffolds for Neural Stem Cell Culture and Differentiation -- The Delivery and Evaluation of RNAi Therapeutics for Heterotopic Ossification Pathologies -- Mimicking Bone Microenvironment for Directing Adipose Tissue-Derived Mesenchymal Stem Cells into Osteogenic Differentiation -- Cultivation of Human Bone-Like Tissue from Pluripotent Stem Cell-Derived Osteogenic Progenitors in Perfusion Bioreactors
    Abstract: Biomimetics and Stem Cells: Methods and Protocols collects a series of approaches to demonstrate the role and value of biomimetics for the better understanding of stem cell behavior and the acceleration of their application in regenerative medicine. Recent advances in tissue engineering are enabling scientists to ́€˜́€˜instruct́€™́€™ stem cells toward differentiating into the right phenotypes, in the right place and at the right time. Given these advances, biomimetic environments are being designed to recapitulate, in vitro, the combinations of factors known to guide tissue development and regeneration in vivo and thereby help unlock the full potential of the stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Practical and essential, Biomimetics and Stem Cells: Methods and Protocols focuses on the use of biomimetic systems for stem cells in order to aid in moving this vital field of study forward
    Pages: XI, 187 p. 54 illus., 31 illus. in color. : online resource.
    ISBN: 9781493913329
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  • 2
    ISSN: 1573-9686
    Keywords: Image analysis ; Tissue engineering ; Bioreactor ; Safranin O
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Cartilage tissue engineering can provide a valuable tool for controlled studies of tissue development. As an example, analysis of the spatial distribution of glycosaminoglycans (GAG) in sections of cartilaginous tissues engineered under different culture conditions could be used to correlate the effects of environmental factors with the structure of the regenerated tissue. In this paper we describe a computer-based technique for quantitative analysis of safranin-O stained histological sections, using low magnification light microscopy images. We identified a parameter to quantify the intensity of red color in the sections, which in turn was proportional to the biochemically determined wet weight fraction of GAG in corresponding tissue samples, and to describe the spatial distribution of GAG as a function of depth from the section edge. A broken line regression model was then used to determine the thickness of an external region, with lower GAG fractions, and the spatial rate of change in GAG content. The method was applied to the quantitatation of GAG distribution in samples of natural and engineered cartilage, cultured for 6 weeks in three different vessels: static flasks, mixed flasks, and rotating bioreactors. © 1999 Biomedical Engineering Society. PAC99: 8780Rb, 8715Mi, 8763Lk
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature biotechnology 23 (2005), S. 1237-1239 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Tissue engineering aims to grow tissue replacements by combining cells and matrices under defined laboratory conditions. The shortage of donor tissues and organs and the increasing demand for tissue repair from an aging population have catalyzed research in the field. A US National Institutes of ...
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Synthetic polymer scaffolds designed for cell transplantation were reproducibly made on a large scale and studied with respect to biocompatibility, structure and biodegradation rate. Polyglycolic acid (PGA) was extruded and oriented to form 13 μm diameter fibers with desired tenacity. Textile ...
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1520-6033
    Source: ACS Legacy Archives
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1520-5045
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cartilage constructs were grown using isolated chondrocytes and biodegradable polymer scaffolds made of fibrous polyglycolic acid in the form of 1-cm-dia × 5-mm-thick discs. The scaffolds were seeded in a mixed cell suspension and cultured for up to 8 weeks under static or mixed tissue culture conditions in petri dishes and spinner flasks. Turbulent mixing significantly improved the biochemical compositions and altered morphologies of the cartilage constructs, which were the thickest ones cultured to date in vitro. Constructs from mixed cultures were more regular in shape and contained up to 70% more cells, 60% more sulfated glycosaminoglycan, and 125% more total collagen when compared to constructs from static cultures. Mixing also induced the formation of an outer capsule with multiple layers of elongated cells and collagen fibrils around the inner tissue phase, while statically grown constructs consisted of round cells embedded in cartilaginous matrix. Mixing during cell seeding and tissue culture is thus an important parameter for the cultivation of tissue-engineered cartilage in a range of sizes, shapes and compositions for a variety of clinical applications (e.g., fibrous cartilage for reconstructive surgery or articular cartilage for joint resurfacing).
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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