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  • 1
  • 2
    Publication Date: 2012-06-09
    Description: Voltage-gated sodium (Na(v)) channels are essential for the rapid depolarization of nerve and muscle, and are important drug targets. Determination of the structures of Na(v) channels will shed light on ion channel mechanisms and facilitate potential clinical applications. A family of bacterial Na(v) channels, exemplified by the Na(+)-selective channel of bacteria (NaChBac), provides a useful model system for structure-function analysis. Here we report the crystal structure of Na(v)Rh, a NaChBac orthologue from the marine alphaproteobacterium HIMB114 (Rickettsiales sp. HIMB114; denoted Rh), at 3.05 A resolution. The channel comprises an asymmetric tetramer. The carbonyl oxygen atoms of Thr 178 and Leu 179 constitute an inner site within the selectivity filter where a hydrated Ca(2+) resides in the crystal structure. The outer mouth of the Na(+) selectivity filter, defined by Ser 181 and Glu 183, is closed, as is the activation gate at the intracellular side of the pore. The voltage sensors adopt a depolarized conformation in which all the gating charges are exposed to the extracellular environment. We propose that Na(v)Rh is in an 'inactivated' conformation. Comparison of Na(v)Rh with Na(v)Ab reveals considerable conformational rearrangements that may underlie the electromechanical coupling mechanism of voltage-gated channels.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979295/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979295/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhang, Xu -- Ren, Wenlin -- DeCaen, Paul -- Yan, Chuangye -- Tao, Xiao -- Tang, Lin -- Wang, Jingjing -- Hasegawa, Kazuya -- Kumasaka, Takashi -- He, Jianhua -- Wang, Jiawei -- Clapham, David E -- Yan, Nieng -- P01 NS072040/NS/NINDS NIH HHS/ -- T32 HL007572/HL/NHLBI NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2012 May 20;486(7401):130-4. doi: 10.1038/nature11054.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉State Key Laboratory of Bio-membrane and Membrane Biotechnology, Center for Structural Biology, Tsinghua University, Beijing 100084, China.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22678295" target="_blank"〉PubMed〈/a〉
    Keywords: Alphaproteobacteria/*chemistry ; Amino Acid Sequence ; Bacterial Proteins/*chemistry/metabolism ; Crystallization ; Crystallography, X-Ray ; HEK293 Cells ; Humans ; *Ion Channel Gating ; Models, Molecular ; Molecular Sequence Data ; Protein Conformation ; Sodium Channels/*chemistry/metabolism ; Structure-Activity Relationship
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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  • 3
    Publication Date: 2013-05-24
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ren, Wencai -- Cheng, Hui-Ming -- England -- Nature. 2013 May 23;497(7450):448-9. doi: 10.1038/497448a.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23698442" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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  • 4
    Publication Date: 2014-11-22
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ma, Zhijun -- Melville, David S -- Liu, Jianguo -- Chen, Ying -- Yang, Hongyan -- Ren, Wenwei -- Zhang, Zhengwang -- Piersma, Theunis -- Li, Bo -- New York, N.Y. -- Science. 2014 Nov 21;346(6212):912-4. doi: 10.1126/science.1257258.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200438, China. No. 1261 Dovedale Road, Rural Delivery 2, Wakefield, Nelson, 7096, New Zealand. Center for Systems Integration and Sustainability, Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48823, USA. College of Nature Reserve, Beijing Forestry University, Beijing 100083, China. Chair in Global Flyway Ecology, Centre for Ecological and Evolutionary Studies, University of Groningen, Post Office Box 11103, 9700 CC Groningen, Netherlands. Department of Marine Ecology, Royal Netherlands Institute for Sea Research (NIOZ), Post Office Box 59, 1790 AB Den Burg, Texel, Netherlands. World Wildlife Fund China Shanghai Office, No. 121 Zhongshan North Road, Shanghai 200083, China. Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing 100875, China. zhijunm@fudan.edu.cn. ; No. 1261 Dovedale Road, Rural Delivery 2, Wakefield, Nelson, 7096, New Zealand. ; Center for Systems Integration and Sustainability, Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48823, USA. ; Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200438, China. ; College of Nature Reserve, Beijing Forestry University, Beijing 100083, China. Chair in Global Flyway Ecology, Centre for Ecological and Evolutionary Studies, University of Groningen, Post Office Box 11103, 9700 CC Groningen, Netherlands. Department of Marine Ecology, Royal Netherlands Institute for Sea Research (NIOZ), Post Office Box 59, 1790 AB Den Burg, Texel, Netherlands. ; World Wildlife Fund China Shanghai Office, No. 121 Zhongshan North Road, Shanghai 200083, China. ; Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing 100875, China. ; Chair in Global Flyway Ecology, Centre for Ecological and Evolutionary Studies, University of Groningen, Post Office Box 11103, 9700 CC Groningen, Netherlands. Department of Marine Ecology, Royal Netherlands Institute for Sea Research (NIOZ), Post Office Box 59, 1790 AB Den Burg, Texel, Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25414287" target="_blank"〉PubMed〈/a〉
    Keywords: Animal Migration ; Animals ; *Biodiversity ; Birds ; China ; *Conservation of Natural Resources ; *Wetlands
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
    Publication Date: 2015-07-16
    Description: The major facilitator superfamily glucose transporters, exemplified by human GLUT1-4, have been central to the study of solute transport. Using lipidic cubic phase crystallization and microfocus X-ray diffraction, we determined the structure of human GLUT3 in complex with D-glucose at 1.5 A resolution in an outward-occluded conformation. The high-resolution structure allows discrimination of both alpha- and beta-anomers of D-glucose. Two additional structures of GLUT3 bound to the exofacial inhibitor maltose were obtained at 2.6 A in the outward-open and 2.4 A in the outward-occluded states. In all three structures, the ligands are predominantly coordinated by polar residues from the carboxy terminal domain. Conformational transition from outward-open to outward-occluded entails a prominent local rearrangement of the extracellular part of transmembrane segment TM7. Comparison of the outward-facing GLUT3 structures with the inward-open GLUT1 provides insights into the alternating access cycle for GLUTs, whereby the C-terminal domain provides the primary substrate-binding site and the amino-terminal domain undergoes rigid-body rotation with respect to the C-terminal domain. Our studies provide an important framework for the mechanistic and kinetic understanding of GLUTs and shed light on structure-guided ligand design.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Deng, Dong -- Sun, Pengcheng -- Yan, Chuangye -- Ke, Meng -- Jiang, Xin -- Xiong, Lei -- Ren, Wenlin -- Hirata, Kunio -- Yamamoto, Masaki -- Fan, Shilong -- Yan, Nieng -- Howard Hughes Medical Institute/ -- England -- Nature. 2015 Oct 15;526(7573):391-6. doi: 10.1038/nature14655. Epub 2015 Jul 15.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉State Key Laboratory of Membrane Biology, Tsinghua University, Beijing 100084, China. ; Center for Structural Biology, Tsinghua University, Beijing 100084, China. ; Tsinghua-Peking Center for Life Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China. ; Advanced Photon Technology Division, Research Infrastructure Group, SR Life Science Instrumentation Unit, RIKEN/SPring-8 Center, 1-1-1 Kouto Sayo-cho Sayo-gun, Hyogo 679-5148 Japan. ; Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26176916" target="_blank"〉PubMed〈/a〉
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    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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  • 6
    Publication Date: 2015-01-13
    Description: Complex three-dimensional (3D) structures in biology (e.g., cytoskeletal webs, neural circuits, and vasculature networks) form naturally to provide essential functions in even the most basic forms of life. Compelling opportunities exist for analogous 3D architectures in human-made devices, but design options are constrained by existing capabilities in materials growth and assembly. We report routes to previously inaccessible classes of 3D constructs in advanced materials, including device-grade silicon. The schemes involve geometric transformation of 2D micro/nanostructures into extended 3D layouts by compressive buckling. Demonstrations include experimental and theoretical studies of more than 40 representative geometries, from single and multiple helices, toroids, and conical spirals to structures that resemble spherical baskets, cuboid cages, starbursts, flowers, scaffolds, fences, and frameworks, each with single- and/or multiple-level configurations.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Xu, Sheng -- Yan, Zheng -- Jang, Kyung-In -- Huang, Wen -- Fu, Haoran -- Kim, Jeonghyun -- Wei, Zijun -- Flavin, Matthew -- McCracken, Joselle -- Wang, Renhan -- Badea, Adina -- Liu, Yuhao -- Xiao, Dongqing -- Zhou, Guoyan -- Lee, Jungwoo -- Chung, Ha Uk -- Cheng, Huanyu -- Ren, Wen -- Banks, Anthony -- Li, Xiuling -- Paik, Ungyu -- Nuzzo, Ralph G -- Huang, Yonggang -- Zhang, Yihui -- Rogers, John A -- New York, N.Y. -- Science. 2015 Jan 9;347(6218):154-9. doi: 10.1126/science.1260960.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Materials Science and Engineering and Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. ; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. ; Department of Civil and Environmental Engineering and Department of Mechanical Engineering, Center for Engineering and Health, and Skin Disease Research Center, Northwestern University, Evanston, IL 60208, USA. Department of Civil Engineering and Architecture, Zhejiang University, Hangzhou 310058, P.R. China. ; Department of Materials Science and Engineering and Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Department of Materials Science and Engineering, Department of Energy Engineering, Hanyang University, Seoul 133-791, Republic of Korea. ; Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. ; Department of Civil and Environmental Engineering and Department of Mechanical Engineering, Center for Engineering and Health, and Skin Disease Research Center, Northwestern University, Evanston, IL 60208, USA. Key Laboratory of Pressure Systems and Safety (MOE), School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai 200237, P.R. China. ; Department of Materials Science and Engineering and Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Department of Civil and Environmental Engineering and Department of Mechanical Engineering, Center for Engineering and Health, and Skin Disease Research Center, Northwestern University, Evanston, IL 60208, USA. ; Department of Materials Science and Engineering, Department of Energy Engineering, Hanyang University, Seoul 133-791, Republic of Korea. ; Department of Materials Science and Engineering and Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. ; Department of Civil and Environmental Engineering and Department of Mechanical Engineering, Center for Engineering and Health, and Skin Disease Research Center, Northwestern University, Evanston, IL 60208, USA. jrogers@illinois.edu y-huang@northwestern.edu yihui.zhang2011@gmail.com. ; Department of Civil and Environmental Engineering and Department of Mechanical Engineering, Center for Engineering and Health, and Skin Disease Research Center, Northwestern University, Evanston, IL 60208, USA. Center for Mechanics and Materials, Tsinghua University, Beijing 100084, P.R. China. jrogers@illinois.edu y-huang@northwestern.edu yihui.zhang2011@gmail.com. ; Department of Materials Science and Engineering and Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. jrogers@illinois.edu y-huang@northwestern.edu yihui.zhang2011@gmail.com.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25574018" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
    Publication Date: 2014-10-11
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Qu, Mingzhi -- Lefebvre, Daniel D -- Wang, Yuxiang -- Qu, Yunfang -- Zhu, Donglin -- Ren, Wenwei -- New York, N.Y. -- Science. 2014 Oct 10;346(6206):175-6. doi: 10.1126/science.346.6206.175-b. Epub 2014 Oct 9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, Queen's University, Kingston, ON, K7L 3N6, Canada. ; Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Sciences, Fudan University, Shanghai, 200433, China. ; Jiangsu Engineering Consulting Center, Nanjing, Jiangsu Province, 210003, China. ; Key Laboratory of Yangtze Water Environment, Ministry of Education, The College of Environment Science and Engineering, Tongji University, Shanghai, 200092, China. wenwei.ren.tongji@gmail.com.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25301608" target="_blank"〉PubMed〈/a〉
    Keywords: China ; Cyanobacteria/*growth & development ; *Harmful Algal Bloom ; Lakes/*microbiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 80 (2002), S. 3174-3176 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Electric-field-induced phase transitions and piezoelectric properties of 〈001〉-oriented Pb(Zn1/3Nb2/3)O3–xPbTiO3 single crystals for x=4.5% and 8% (PZN–4.5%PT and PZN–8%PT) have been investigated as a function of temperature. It was found that the piezoelectric properties and phase transitions for both crystals are strongly dependent on temperature. The measurements of polarization and strain as a function of unipolar electric field show that the field for the rhombohedral–tetragonal phase transition decreases linearly with temperature in the range between 25 and 105 °C. Thus, raising the temperature can stabilize the tetragonal phase in 〈001〉-oriented PZN–PT crystals. Longitudinal piezoelectric constant d33 in the rhombohedral phase increases with temperature for both crystals; in PZN–4.5%PT the slope itself increases significantly at temperatures over 60 °C. In the field-induced tetragonal phase, there is little change in d33 for PZN–8%PT within the temperature range investigated, while in PZN–4.5%PT a slight increase of d33 with temperature was observed at temperatures above 80 °C. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Stable polymers with nonlinear optical side groups were synthesized from maleic anhydride copolymers and the azo dye Disperse Red 1 via esterification. After electrode poling under 135 V/μm at 185 °C, thermal stabilities were measured by pyroelectric and electro-optic thermal analysis and compared to a guest/host polymer of polymethylmethacrylate and the same dye. The pyroelectrically detected dipole orientation and the electro-optic activity of the side-chain polymers decreased only above 150 °C, and electro-optic r33 coefficients of up to 6 pm/V were achieved at 780 nm even without optimizing the dye content.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract The aim of this investigation was to determine the relationship between loss of radiographic crestal bone height and probing attachment loss. As part of this aim, we are introducing a new method for determination of a threshold for significant probing attachment loss which takes into account the error contributed by alteration in tissue tone. 57 adults with established periodontitis were selected. Radiographs were taken using the Rinn alignment system. Crestal change was determined from bone height measurements on digitized images of pairs of radiographs using the “side-by-side” technique of analysis developed recently by us. Probing attachment loss was measured using the Florida electronic probe system. Radiographic and probing measurements were made at baseline and after 1 year. No treatment was given during this period. A direct and significant relationship was observed between radiographic bone loss and probing attachment loss on a site basis (p= 0.0001, r2= 0.018) and between subject means (p= 0.0014, r2= 0.16). Radiographic and probing attachment change at all categories of sites, dichotomously classified as to not changing or loosing indicated 13% of sites were loosing by measurement of radiographic change and 9.6% were Loosing by measurement of attachment change. Concordance in radiographic and attachment level change was found in 82% of sites examined. The relative diagnostic import of change in probing attachment or change in radiographic bone height requires treatment outcome studies based on use of diagnostic information of the 2 measuring techniques used singly and in combination.
    Type of Medium: Electronic Resource
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