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  • 1
    Keywords: Biochemistry ; Protein Science ; Springer eBooks
    Description / Table of Contents: Making the Case for Functional Proteomics -- Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors -- Two-dimensional 16-BAC/SDS polyacrylamide gel electrophoresis of mitochondrial membrane proteins -- Systematic Glycolytic Enzyme Activity Analysis from Human Serum with PEP Technology -- A protein decomplexation strategy in snake venom proteomics -- Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level -- A systematic analysis workflow for high-density customized protein microarrays in biomarker screening -- Metaproteomics study of the gut microbiome -- Double-One Dimensional Electrophoresis (D1-DE) Adapted for Immunoproteomics -- BioID: A proximity dependent labeling approach in proteomics study -- Functional application of snake venom proteomics in in vivo antivenom assessment -- Proteomic detection of carbohydrate-active enzymes (CAZymes) in microbial secretomes -- An Overview of Mass Spectrometry-based Methods for Functional Proteomics -- Functional proteomic analysis to characterize signaling crosstalks -- Identification of unexpected protein modifications by mass spectrometry based proteomics -- Label-free LC-MS/MS Strategy for Comprehensive Proteomic Profiling of Human Islets Collected using Laser Capture Microdissection from Frozen Pancreata -- Mass Spectrometry based targeted proteomics for cell and tissue analysis -- Metabolomic investigation of Staphylococcus aureus antibiotic susceptibility by liquid chromatography coupled to high resolution mass spectrometry -- Nuts and bolts of protein quantification by on-line trypsin digestion coupled LC-MS/MS analysis -- Proteases: Pivot Points in Functional Proteomics -- The Use of Combinatorial Hexapeptide Ligand Library (CPLL) in Allergomics -- Efficient Extraction and Digestion of Gluten Proteins -- Glycosylation profiling of tumor marker in plasma using bead-based immunoassay -- Protein-specific analysis of invertebrate glycoproteins -- The use of proteomics studies in identifying moonlighting proteins -- Two-dimensional biochemical purification for global proteomic analysis of macromolecular protein complexes -- A data analysis protocol for quantitative data-independent acquisition proteomics
    Abstract: This book seeks to fill in the current technology gap with a specific collection of technologies developed for the study of protein function at a proteome scale. Chapters explore topics from protein functions to other aspects of protein analysis, especially in post-translational modification, as most proteomes use this mechanism in some capacity to carry out their unique role in cellular regulation. By comparing functional proteomes, this presents a bridge to other levels of system biology research including genomics and metabolomics in order to provide readers with a relatively complete picture for how one might study the biological system of their interest. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Functional Proteomics: Methods and Protocols collects these novel technologies in the hope that new frontiers in biological research will be created, important drug targets can be identified, and clinically validated biomarkers and diagnostic tests can be further developed
    Pages: XII, 477 p. 89 illus., 63 illus. in color. : online resource.
    ISBN: 9781493988143
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  • 2
    ISSN: 1435-5922
    Keywords: Key words:Clostridium difficile ; PCR ; cytotoxicity ; definitive diagnosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: Clostridium difficile causes pseudomembranous colitis and antibiotic-associated diarrhea. The definitive diagnosis of C. difficile infection is finally accomplished by the isolation of toxigenic C. difficile. However, only a small number of Japanese clinical laboratories are able to reach a definitive diagnosis of C. difficile infection, probably because simple reliable assays for toxins in the isolates are not available. In this study, we examined the compatibility of a polymerase chain reaction (PCR) assay and tissue culture assay to identify toxigenic C. difficile, in toxigenic and nontoxigenic C. difficile isolates from Japanese patients and healthy carriers. The specificity of PCR primers was demonstrated by restriction endonuclease digestion and seminested PCR in C. difficile VPI 10463 strain. No PCR product was amplified in the eight other clostridial species used to check the specifiecty of the PCR assay. The detection limit was 103 cells. Both toxin A and toxin B genes (the genes encoding the major virulence factors of C. difficile) were detected in 58 toxigenic C. difficile isolates, which showed a wide range of cytotoxic activity in tissue culture assays. Neither of the toxin genes was carried by 40 nontoxigenic strains of C. difficile. The results of this study strongly suggest that a definitive diagnosis of C. difficile infection can be accomplished by PCR detection of the toxin genes rather than by tissue culture assay of isolates.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1662-9779
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Physics
    Notes: A novel hollow sphere having rigid binaphthyl macrocycle as shell was prepared by meansof sacrifice the silica core. The synthesis of hollow sphere from rigid colloidal silica particles occursin three steps: a) modification of silica particles with vinyltriethoxysilane as coupling agent, b)immersion in the solution of monomer having rigid binaphthyl macrocycle and polymerization, and c)removal of silica particles. These macrocycles contained in the shell of hollow spheres belong to animportant class of host-guest macrocyclic material in which the rigid backbone and C2 symmetry ofthe binaphthyl unit play an important role in complexing guest molecules. This will endow hollowsphere with new opportunities in molecular recognition and separation.The morphology of colloidalsilica particles and hollow spheres was characterized by SEM and TEM
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Solid state phenomena Vol. 121-123 (Mar. 2007), p. 429-432 
    ISSN: 1662-9779
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Physics
    Notes: Multi morphology of polyaniline (PANI), nanotubes, micrograins, solid and hollowspheres, were synthesized by aqueous oxidative polymerization of aniline in the presence of sodiumhydroxide (NaOH), which was proposed as an alkali-guided method by the authors. By changing themolar ratio of aniline monomer to alkali, with a constant pH, the morphology can be controlled fromnanotubes to micrograins, to solid spheres and to hollow spheres which were proved by SEM andTEM. The backbone structure was characterized by UV-VIS, FTIR and XRD
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We examined the hypothesis that hyperhomocyst(e)inaemia in the maternal or fetal circulation is associated with placental vascular disease with either the maternal syndrome of pre-eclampsia and/or fetal syndrome of growth restriction. Maternal plasma homocyst(e)ine levels were significantly higher in pregnancies complicated by pre-eclampsia, pregnancies with evidence of umbilical placental vascular disease, and pregnancies with both complications compared with the normal pregnancy group. In the fetal circulation mean plasma homocyst(e)ine concentration was significantly higher in the pre-eclampsia group compared with the normal group. The results suggest that hyperhomocyst(e)inaemia may be a risk marker for placental vascular disease and maternal pre-eclampsia. The elevated fetal plasma homocyst(e)ine concentrations, found only in the group of pregnancies with pre-eclampsia in the absence of umbilical placental vascular disease, may be due to an effect of placental vascular disease on homocyst(e)ine transfer from the maternal to fetal circulation.
    Type of Medium: Electronic Resource
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  • 6
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 7
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1468-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A gas chromatography coupled with flame ionization detection (GC-FID) and mass spectrometric detection (MSD) method was developed to determine the six kinds of phthalate esters [dimethyl phthalate (DMP), diethyl phthalate (DEP), di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di(2-ethylhexyl) phthalate (DEHP) and di-n-octyl phthalate (DOP)] in cosmetics (solid, cream and liquid cosmetics). The cosmetics were extracted with methanol by ultrasonic and then separated with high-speed centrifugation. The upper clear layer was dried and filtered through a 0.45 μm pore diameter filter. The filtrate was injected into GC-FID/GC-MS for detection. GC-FID chromatogram was applied for qualitative analysis, external standard method was used for quantitative analysis. Confirmation of phthalate presence was undertaken by GC-EI-MS. The recovery range of all phthalates were between 92.0 and 110.0% with relative standard deviations between 1.95 and 5.92%. The low detection limits of the method were: 0.1 ng for DMP, DEP, DBP and BBP, 0.5 ng for DEHP and DOP. The method had advantages of high precision and sensitivity, simplicity of pretreatment. The method can be used to test the six kinds of phthalate esters in cosmetics.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0006-3592
    Keywords: purification ; inositol monophosphatase ; lithium ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: myo-Inositol monophosphate phosphatase (IMPase) has been purified 888-fold to apparent homogeneity from procine brains. The purification procedure involves: homogenization, ammonium sulfate fractionation, and a number of ion-exchange and gel-filtration chromatography steps. The purified enzyme exhibited a final specific activity of 932 nmol · min-1 · mg-1. The molecular mass of the enzyme was estimated to be 29kDa by SDS poly-acrylamide gel electrophoresis and 58 ± 5 kDa by HPLC gel filtration in 10mM Tris-HCI, pH 7.4. Kinetic measurements have shown that the apparent Km value of the phosphatase for the utilization of inositol-1-phosphate and β-glycerol phosphate are 3.20 × 10-4 and 8 × 10-3 M, respectively. Similar to the same enzyme isolated from bovine brains, the porcine brain enzyme has been shown to be inhibited by lithium. The K1 was determined to be 6.38 × 10-4 M and the inhibition is uncompetitive. © 1995 John Wiley & Sons, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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