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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Digestive diseases and sciences 43 (1998), S. 2168-2168 
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The determination of whether metabolic homogeneity exists in an organ is of importance to both the clinician and investigator. These studies examine synthesis of DNA, RNA, protein, and oxygen uptake in the three anatomical segments of the rat pancreas. There was rather close agreement among the three segments insofar as these metabolic measurements were concerned. We concluded that there is metabolic uniformity in the rat pancreas and that measurements obtained in one segment reflect activities in the other two segments.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Digestive diseases and sciences 19 (1974), S. 459-464 
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Several epidemiologic observations concerning pancreatic carcinoma appear well established. There are: (1) carcinoma of the pancreas is increasing in incidence; (2) incidence equals mortality despite improved diagnostic methods. Several etiologic observations appear established. These are: (1) chemical agents induce carcinoma of the pancreas in animals; (2) human exposed to industrial chemicals have an increased incidence of pancreatic carcinoma; (3) patients with diabetes mellitus and calcific pancreatitis may have an increased incidence of pancreatic carcinoma. It is possible that the increased incidence of pancreatic carcinoma reflects greater exposure to environmental carcinogens, or an increase in the frequency of diseases associated with pancreatic carcinoma.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Digestive diseases and sciences 19 (1974), S. 37-42 
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Epidemiologic studies show an increase in the incidence of pancreatic carcinoma and suggest that this increase may result, at least in part, from increased exposure to chemical carcinogens. Little information is available concerning the uptake of such agents or their effects on the pancreas. For these studies a well-recognized and commonly studied chemical carcinogen, 3-methylcholanthrene (3-MC), was injected intraperitoneally into rats to determine to what extent rat pancreas sequesters and metabolizes this drug. The carcinogen was taken up in the pancreas to the same degree as the liver on a per gram wet weight basis. The majority of binding was to the membranous components with only 17% remaining in the supernatant fraction. Maximal incorporation occurred around 36 hours following administration. Ether extracts of homogenates revealed that few breakdown products were formed even up to 48 hours after injection. It appears that rat pancreas sequesters 3-MC but has little metabolizing capacity.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This is a review of current information concerning mechanisms involved in transport and secretion of macromolecules in exocrine glands. Emphasis has been placed on information available for pancreatic acinar cells. The review was prompted by the availability of considerable amounts of new information developed during the past several years. Exportable proteins in the pancreatic acinar cells are synthesized on ribosomes attached to the endoplasmic reticulum. Following synthesis, nascent proteins are transported from ribosomes attached to the endoplasmic reticulum into intracisternal spaces bound by the endoplasmic reticulum. The proteins are then carried to the Golgi complex by transitional elements. Zymogen granules are formed in the Golgi complex and migrate to the cell apex. Appropriate stimulation leads to fusion of the zymogen granule membrane and apical plasmalemma followed by a break in the membrane and consequent release of the granule content into the ductules. The exact molecular events involved in the process of secretion are not known. The roles of cAMP and cGMP in pancreatic secretion are supported by indirect evidence only. The role of calcium in secretion is apparent, but further investigation is needed to delineate the exact mechanism of its action. Membrane depolarization and associated ionic fluxes seem to play a significant role.
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  • 6
  • 7
    ISSN: 0173-0835
    Keywords: Trypanosoma brucei ; Free-flow electrophoresis ; Endosomes ; Lysosomes ; Transferrin ; Percoll ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: In this paper we demonstrate the power of preparative free-flow electrophoresis (FFE) for the study of endocytosis by African trypanosomes. Endocytosis of extracellular macromolecules by these parasites occurs through a specialized region of the parasite called the flagella pocket. The uptake of fluid phase markers such as horseradish peroxidase (HRP) into the various compartments of the endocytic pathway of bloodstream forms of Trypanosoma brucei brucei was manipulated by regulating the external environment (e.g., by altering the temperature of incubation). The various subcellular compartments were then separated by free-flow electrophoresis (FFE) or isopycnic density gradient centrifugation and analyzed for marker uptake. At low temperatures, HRP was found predominantly in the flagellar pocket. Increasing the temperature resulted in a time-dependent uptake of HRP into more positively charged endosomal fractions. However, little HRP activity was detected in lysosomal compartments, suggesting that either HRP had not yet entered the lysosome or was degraded immediately upon entry. Through the use of FFE we were able to identify and analyze compartments of the endosomal pathway that were not possible to identify by density gradient centrifugation alone. Although the differences in FFE separation of the endocytic compartments as seen in HRP uptake were striking, the minor changes seen within the lysosomal system were more subtle, as depicted in the protease profiles. In conlusion, we show that preparative FFE is a powerful technique for the analysis and separation of flagellar pocket-derived membranes from other endosomal and lysosomal compartments of African trypanosomes.
    Additional Material: 7 Ill.
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  • 8
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Endocytosis of antigen by antigen-presenting cells results in the production of peptides that bind to newly synthesized class II molecules of the major histocompatibility complex. A new population of class II-enriched vesicles has been discovered in B lymphocytes that accumulate internalized ...
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  • 9
    ISSN: 1072-8368
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The addition of trifluoroethanol or hexafluoroisopropanol converts the apparent two-state folding of acylphosphatase, a small α/β protein, into a multistate mechanism where secondary structure accumulates significantly in the denatured state before folding to the native state. This ...
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  • 10
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . A procedure was developed to purify a coated vesicle fraction from the protozoan parasite Trypanosoma brucei. Electron microscopy revealed a difference between T. brucei coated vesicles and clathrin-coated vesicles from other eukaryotes: trypanosome vesicles were larger (100 to ISO nm in diameter) and contained an inner coat of electron-dense material in addition to the external coat. Evidence suggests that the internal coat is the parasite's variant surface glycoprotein (VSG) coat. The SDS-PAGE analysis shows the major protein of T. brucei coated vesicles has a molecular mass of 61 kD, similar to VSG; this protein was recognized in an immunoblot by anti-VSG serum. Trypanosome coated vesicles also contain a protein which comigrates with the major protein (clathrin) of coated vesicles purified from rat brains. However, this protein is a minor component and it is not serologically cross-reactive with mammalian clathrin. Immunoblot analysis demonstrated that the parasite vesicles contained host IgG, IgM, and serum albumin.
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