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  • 1
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The p100 gene coding for the p100 protein of Borrelia burgdorferi strain PKo has been cloned, sequenced and expressed in Escherichia coli. An open reading frame including upstream and downstream sequences with potential translation and transcription signals could be identified. The reading frame consists of 1989 nucleotides corresponding to a protein of 663 amino acids and a calculated molecular mass of 75.8 kDa. The protein has a leader peptide and is processed without modification at the N-terminus. A high percentage of amino acid sequence identity could be found to the high-molecular mass protein p83/p93 of B. burgdorferi strain B31.
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  • 4
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A total of 305 Ixodes ricinus ticks (243 nymphs and 62 adults) were collected from three different regions of Thuringia in Middle Germany which are known to be endemic for Borrelia burgdorferi. Our aim was to investigate the carrier rate of ticks for granulocytic Ehrlichia species. The presence of ehrlichial 16S ribosomal DNA was investigated by polymerase chain reaction. Using primers specific for the Ehrlichia phagocytophila group PCR fragments of 151 bp and 943 bp, respectively, were produced in positive samples. Adult ticks showed a significantly higher infection rate (4/62; 6.5%) compared to nymphs (3/243; 1.2%). Prevalence rates varied between 0 and 3.8% regarding the different areas under investigation. The nucleotide sequences showed high similarity (between 97.5% and 99% identity) to the known sequences of the three E. phagocytophila group members HGE agent, E. phagocytophila and Ehrlichia equi. The sequence data did not allow a final classification to a particular member of this group.
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  • 5
    ISSN: 0173-0835
    Keywords: Borrelia burgdorferi ; Pulsed field gel electrophoresis ; Large restriction fragment patterns ; Plasmid profile ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The large restriction fragment patterns (LRFP) and linear plasmid profiles of eight tick isolates of Borrelia burgdorferi sensu lato were investigated with pulsed-field gel electrophoresis (PFGE). The whole cell lysate was examined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The MluI LRFP differentiates two species of Borrelia burgdorferi sensu lato (Borrelia burgdorferi sensu stricto and Borrelia garinii). LRFP is a suitable method to demonstrate genetic hetero- or homogeneity of isolates within one species without subsequent hybridization utilizing diverse probes. Different strains with similar or identical LRFP can be further discriminated by plasmid profile analysis. Our results show that each strain analyzed had a different plasmid profile. Therefore the linear plasmid profile has a potential application as a strain typing procedure. SDS-PAGE of whole-cell lysate supports the findings of homology within the B. burgdorferi sensu stricto species and the heterology within the B. garinii species.
    Additional Material: 3 Ill.
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  • 6
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Zwei Patienten mit Erythema-migrans-Krankheit (EMK) wurden in den Jahren 1973 bzw. 1977 mit oralem Penicillin behandelt. Fünf und sieben Jahre später trat in beiden Fällen nach Zeckenstich eine EMK zum zweiten Mal auf. Die ersten und zweiten Erythema-migrans-Herde waren jeweils an verschiedenen Lokalisationen zu beobachten. Die Testungen auf Antikörper gegenüberBorrelia burgdorferi an Hand von Seren, die vor (in einem Fall), während und nach der zweiten EMK entnommen wurden, waren negativ (Patient 1) oder ergaben einen Anstieg der IgG-Titer um mindestens vier Stufen, ohne jedoch positiv zu werden (Patient 2). Diese Beobachtungen weisen darauf hin, daß eine Reinfektion bei der EMK ohne signifikant erhöhte Antikörpertiter möglich ist.
    Notes: Summary Two patients received oral penicillin for erythema migrans disease (EMD) in 1973 and 1977. Five and seven years later, respectively, they developed EMD for a second time after being bitten by ticks. The first and second erythema migrans lesions appeared in different places. Tests of sera evaluated for antibodies toBorrelia burgdorferi before (in one case), during and after the second episode of EMD remained negative (patient 1) or yielded an at least four-fold rise of IgG antibody titers, but did not become positive (patient 2). These cases provide evidence that reinfection in EMD may occur without a significant elevation of specific antibody titers.
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  • 7
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei einer randomisierten Multicenter-Therapiestudie erhielten 32 Patienten mit Erythema migrans (EM) 10 Tage lang täglich 500 mg Azithromycin und 33 Patienten 3x täglich 1 Mill. IE Penicillin V. Die Nachbeobachtungszeit betrug 17 (3–32) Monate. Die klinischen Erscheinungen heilten bei 20 (62%) der mit Azithromycin und 17 (51%) der mit Penicillin V behandelten Patienten innerhalb von vier Wochen und bei 25 (78%) der mit Azithromycin beziehungsweise 28 (85%) der mit Penicillin V behandelten Patienten innerhalb von drei Monaten nach Therapiebeginn vollständig ab, ohne erneut zu einem späteren Zeitpunkt aufzutreten (kein signifikanter Unterschied). Die klinischen Folgeerscheinungen waren nur leichter Art. Es handelte sich z. B. um Arthralgien, Kopfschmerzen, Abgeschlagenheit, Nackensteife und Paraesthesien. Durch Azithromycin kam es zu einer signifikant schnelleren Abheilung des EM als durch Penicillin V (p〈0.001). Signifikant mehr Patienten mit ausgeprägteren als mit milden Initialerscheinungen hatten einen erhöhten IgM-Antikörper-Titer vor Therapiebeginn (p〈0.001). Meistens gering- bis mäßiggradige Nebenwirkungen fanden sich bei 12 mit Azithromycin und fünf mit Penicillin V behandelten Patienten (p〈0.05). Azithromycin ist offensichtlich ähnlich wirksam wie Penicillin V bei der Behandlung der frühen Lyme-Borreliose, aber Azithromycin scheint das EM schneller abzuheilen.
    Notes: Summary In a randomized multicenter therapeutic trial, 32 patients with erythema migrans received oral azithromycin 500 mg once daily and 33 patients received phenoxymethylpenicillin (penicillin V) 1 million U three times daily for 10 days. Follow-up was for a median of 17 (range 3–32) months. Four weeks after initiation of therapy, 20 (62%) patients given azithromycin and 17 (51%) patients given penicillin V were completely free of all signs and symptoms and did not develop new ones subsequently (no significant difference). Three months after initiation of therapy, the corresponding figures were 25 (78%) azithromycin and 28 (85%) penicillin V recipients (no significant difference). There were only minor sequelae such as arthralgia, headache, fatigue, stiff neck and dysesthesia. Azithromycin led to a significantly faster resolution of the erythema migrans than penicillin V (p〈0.001). Significantly more patients with more severe compared with mild initial disease had an elevated IgM antibody titer prior to therapy (p〈0.001). Usually mild to moderate side effects occurred in 12 patients given azithromycin and five patients given penicillin V (p〈0.05). Azithromycin appears to be as effective as penicillin V for the treatment of early Lyme borreliosis and it seems to clear the erythema migrans more promptly.
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  • 8
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Mit Hilfe von Dunkelfeld- und elektronenmikroskopischer Darstellungen wurden Borrelien-Stämme auf morphologische Varianten untersucht. Es interessierte das Vorkommen und die Bedeutung von atypischen Formen und Sphäroplasten bei antibiotisch behandelten und unbehandelten Patienten mit der klinisch diagnostizierten aktiven Lyme Borreliose bzw. beim Verdacht auf Lyme Borreliose. Ferner untersuchten wir die Zellveränderungen unter Penicillin G-Wirkungin vitro. Die morphologische Analyse zeigte neben der helikalen Form sehr heterogene Veränderungen unter den Borrelien. Die morphologische Veränderung unter Penicillineinwirkung war stark von der Einwirkungszeit abhängig. Die Borrelien-Persister, isoliert von Patienten nach Antibiotikatherapie, zeigten jeweils in großer Zahl (60–80%) atypische Formen. Pleomorphismus, elongierte Zellen und sphäroide Strukturen, Bewegungs- und Vermehrungsunfähigkeit, schlechtes Wachstum im MKP-Medium, sowie Mykoplasmaähnliche Kolonien auf PMR-Agar lassen beiBorrelia burgdorferi die Bildung von Sphäroplasten-L Phase-Varianten vermuten. In Hinblick auf den Krankheitsverlauf der Lyme Borreliose wären diese zellwandfreien Formen eine Möglichkeit für die Borrelien, längere Zeit im Organismus zu überdauern. Die zellwandabhängigen Antikörper können so verschwinden und wieder auftauchen, wenn die Organismen revertieren.
    Notes: Summary As clinical persistence ofBorrelia burgdorferi in patients with active Lyme borreliosis occurs despite obviously adequate antibiotic therapy,in vitro investigations of morphological variants and atypical forms ofB. burgdorferi were undertaken. In an attempt to learn more about the variation ofB. burgdorferi and the role of atypical forms in Lyme borreliosis, borreliae isolated from antibiotically treated and untreated patients with the clinical diagnosis of definite and probable Lyme borreliosis and from patient specimens contaminated with bacteria were investigated. Furthermore, the degeneration of the isolates during exposure to penicillin Gin vitro was analysed. Morphological analysis by darkfield microscopy and scanning electron microscopy revealed diverse alterations. Persisters isolated from a great number of patients (60–80%) after treatment with antibiotics had an atypical form. The morphological alterations in culture with penicillin G developed gradually and increased with duration of incubation. Pleomorphism, the presence of elongated forms and spherical structures, the inability of cells to replicate, the long period of adaptation to growth in MKP-medium and the mycoplasma-like colonies after growth in solid medium (PMR agar) suggest thatB. burgdorferi produce spheroplast-L-form variants. With regard to the polyphase course of Lyme borreliosis, these forms without cell walls can be a possible reason whyBorrelia survive in the organism for a long time and the cell-wall-dependent antibody titers disappear and emerge after reversion.
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  • 9
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In einer prospektiven, randomisierten Multizenter-Studie zur Therapie des Erythema migrans wurden 40 Patienten mit täglich 1 g Ceftriaxon intramuskulär über fünf Tage und 33 Patienten mit täglich 3 × 1 Mill. I. E. Phenoxymethylpenicillin über 12 Tage behandelt. Die Nachbeobachtung betrug durchschnittlich 10±5 Monate. Acht mit Penicillin (24%) und sechs mit Ceftriaxon behandelte Patienten (15%) entwickelten leichtere spätere Manifestationen. Zwei mit Ceftriaxon und ein mit Penicillin Behandelter wiesen erhöhte IgG Antikörper-Titer 10 bis 20 Monate nach Therapie auf.Borrelia burgdorferi konnte bei 29 von 56 Patienten (52%) vor und bei einem Patienten der Penicillingruppe, aber bei keinem Patienten der Ceftriaxongruppe nach Behandlung aus dem Erythema migrans isoliert werden. Ceftriaxon war dem Oral-Penicillin bei einer Untergruppe von Patienten mit mehr als einem Symptom vor Therapie überlegen (p〈0,01), aber nicht bei der Gesamtauswertung klinischer, serologischer und bakteriologischer Erfolgsdaten. Ceftriaxon sollte dem oralen Penicillin bei Patienten mit schwererer früher Lyme-Borreliose vorgezogen werden.
    Notes: Summary In a prospective randomized multicenter trial for the therapy of erythema migrans, 40 patients received ceftriaxone 1 g daily for 5 days and 33 patients obtained phenoxymethylpenicillin, 1 million units 3 times daily, for 12 days. Follow-up was for a mean of 10±5 months. Eight oral penicillin recipients (24%) and six ceftriaxone recipients (15%) developed minor consecutive manifestations. Two ceftriaxone and one penicillin recipient(s) still had elevated IgG antibody titers 10 to 20 months after therapy.Borrelia burgdorferi could be isolated from the erythema migrans in 29 out of 56 patients (52%) before therapy and in one oral penicillin recipient but none of 24 other patients after therapy. Ceftriaxone was superior to oral penicillin in a subgroup of patients with more than one symptom prior to therapy (p〈0.01), but not in the overall evaluation of clinical, serological and bacteriological outcome data. Ceftriaxone ought to be preferred to oral penicillin in patients with more severe early Lyme borreliosis.
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  • 10
    Electronic Resource
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    Springer
    Infection 17 (1989), S. 104-104 
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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