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  • 1
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Im Hoden von Hund und Katze werden folgende Enzyme histochemisch nachgewiesen: NADH-Tetrazoliumreduktase (NADH-T-Red), NADPH-Tetrazoliumreduktase (NADPH-T-Red), Cytochromoxydase (Cyt-Ox), Lactat-Dehydrogenase (LDH), Aldolase (ALD), Alkohol-Dehydrogenase (ADH), Glycerin-1-phosphat-Dehydrogenase (GDH), Glucose-6-phosphat-Dehydrogenase (G-6-PDH), Succinat-Dehydrogenase (SDH), NAD-spezifische Isocitrat-Dehydrogenase (NAD-ICDH). Die starke Fermentaktivität der G-6-PDH und der LDH in den Leydig-Zellen beider Spezies, der relativ hohe Gehalt an histochemisch nachweisbarer ADH in den Zwischenzellen der Katze sowie eine deutliche Reaktion auf GDH in den Sertoli-Zellen der Katze werden diskutiert.
    Notes: Summary In the testes of dog and cat the distribution pattern of NADH-tetrazolium reductase, NADPH-tetrazolium reductase, cytochrome oxydase, lactate dehydrogenase, aldolase, alcohol dehydrogenase, α-glycerophosphate dehydrogenase, glucose-6-phosphate dehydrogenase, succinate dehydrogenase and NAD specific isocitrate dehydrogenase was studied by histochemical means. The strong reaction of G-6-PDH and LDH in the Leydig cells of both species, the relatively high amount of ADH in the interstitial cells of the cat testis and the principal site of α-GPDH in the Sertoli cells of the cat are discussed.
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  • 2
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Wir untersuchten das Verteilungsmuster von unspezifischer Esterase, alkalischer Phosphatase, Adenosintriphosphatase, 5′-Nucleotidase und β-D-Glucuronidase im Hoden von Hund und Katze. Besonders hervorzuheben sind eine starke Aktivität der unspezifischen Esterase in den Sertolizellen der Katze, der Reichtum der Membrana propria aller Hodentubuli an alkalischer Phosphatase und Adenosintriphosphatase sowie die kräftige Reaktion auf β-D-Glucuronidase in den Leydigzellen beider Tierarten. Die Befunde werden diskutiert.
    Notes: Summary The localization of unspecific esterase, alkaline phosphatase, adenosine triphosphatase, 5′-nucleotidase, and β-D-glucuronidase in the testes of cat and dog was demonstrated by histochemical means. We observed a strong esterase activity in the Sertoli cells of the cat and high amounts of alkaline phosphatase and adenosine triphosphatase in the membrana propria of all seminiferous tubules. In both species the principal site of β-D-glucuronidase was in the Leydig cells. Our findings obtained being discussed.
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  • 3
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In der Harderschen Drüse des Kaninchens wurden mit der Tetrazoliumtechnik die Enzyme Aldolase und Alkohol-Dehydrogenase histochemisch nachgewiesen und ihr Verteilungsbild in den beiden Drüsenlappen studiert. Beide Drüsenanteile zeigen hohe Aktivitäten der genannten Enzyme. Auf Grund dieser und früher mitgeteilten Befunde (Kühnel, 1966) wird die Möglichkeit nicht ausgeschlossen, daß die Hardersche Drüse an der Biosynthese von Steroiden beteiligt ist.
    Notes: Summary The histochemical localization of aldolase (ALD) and alcohol-dehydrogenase (NAD-ADH) was studied in both lobes of the Harder gland in rabbits by means of the tetrazolium technique. Both lobes of the Harder gland were active. The enzyme activities of ALD and ADH and those of G-6-PDH and NAD-T-Red and their functional link with the process of steroidbiosynthesis are discussed.
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  • 4
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In den Glandulae inguinales 4 geschlechtsreifer Kaninchen (2 männliche, 2 weibliche) werden mehrere Enzyme der Glykolyse und ihrer Seitenwege, des Citronensäure-Zyklus und der Atmungskette histochemisch nachgewiesen. Es zeigt sich, daß sowohl in der Histotopik als auch in der Aktivität dieser Enzyme Parallelen zu dem Enzymverteilungsbild in apokrinen Duft- und Talgdrüsen der Haut bestehen. Die Befunde werden diskutiert.
    Notes: Summary In the inguinal glands of 4 adult rabbits (2 males, 2 females) the distribution pattern of some dehydrogenases and of cytochrome oxydase is described. In the brown lobe of the inguinal gland activity and localization of the enzymes studied parallel the condition found in mammalian apocrine scent glands. The white lobe of the inguinal gland is according to morphological and histochemical features an enlarged and specialized sebaceous gland.
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  • 5
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In den Glandulae inguinales 4 geschlechtsreifer Kaninchen (2♂, 2♀) wurden folgende Enzyme mit histochemischen Methoden nachgewiesen: Alkalische Phosphatase, Adenosintriphosphatase, Glucose-6-phosphatase, 5′-Nukleotidase, unspezifische Esterase, β-D-Glucuronidase und β-D-Galactosidase. Die Histotopik dieser Enzyme in der braunen und weißen Inguinaldrüse des Kaninchens zeigt in zahlreichen Punkten eine weitgehende Übereinstimmung mit dem Enzymverteilungsbild in den Schweiß- und Talgdrüsen der Haut. Die Befunde werden diskutiert.
    Notes: Summary In the glandulae inguinales of 4 adult rabbits (2 male, 2 female) the distribution of alkaline phosphatase, adenosintriphosphatase, glucose-6-phosphatase, 5′-nucleotidase, unspecific esterase, β-D-glucuronidase and β-D-galactosidase was studied by histochemical means. The site of enzymatic activity in the white and in the brown inguinal gland of the rabbit shows a condition very similar to that found in the sweat glands and the sebaceous glands of the skin. The findings obtained being discussed.
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  • 6
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The innervation pattern of the bovine deferent duct was studied by acetylcholinesterase (AChE)-histochemistry and by immunohistochemical methods. Using antibodies against protein gene product-9.5 (PGP-9.5) and neuron specific enolase (NSE) the complete innervation pattern can be visualized. Thick nerve bundles in the periductal connective tissue supply the two-layered muscular coat. The inner, mainly circularly arranged muscle bundles are innervated by a particularly dense plexus, whereas the nervous network of the more longitudinally running outer musculature is somewhat looser. Additionally, nerve fibres were observed in the subepithelial space in connection with blood vessels and in close proximity to the basal lamina. An innervation pattern analogous to that of the two panneuronal markers was displayed in the immunoreaction against dopamine-beta-hydroxylase (DBH), indicating that the innervation of the bovine deferent duct is predominantly adrenergic. However, the positive reaction with a monoclonal antibody against cholinacetyltransferase (ChAT) specifically demonstrated for the first time the presence of a cholinergic nerve plexus, restricted to the inner muscular layer and the subepithelial space. A modified, direct-colouring AChE-method is presented, which uses copper chloride as source of cupric ions, acetylthiocholine chloride as substrate and 2-morpholinoethanesulphonic acid (MES) as buffer. After short incubation (1–2 h) our modified method allows the specific visualization of cholinergic nerves, comparable to the results of ChAT-immunoreactivity; following a long incubation time (24 h), it reliably illustrates the autonomous innervation pattern as completely as immunohistochemical panneuronal markers.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 125 (1966), S. 177-188 
    ISSN: 1432-0568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Aufteilungsmodus der Arterien, Verhalten der Venen und Anordnung des sympathischen Nervensystems in der Regio sacralis und der proximalen Regio caudalis von Aluatta und den Atelinae lassen erkennen, daß hier ein Zentrum für die Regulierung der Blutversorgung des für diese Tiere biologisch, so bedeutsamen Greifschwanzes zu suchen ist. Die beiden funktionell unterschiedlichen Schwanzstrecken, nämlich die Basis, in der alleine die Dorsalbewegungen des Schwanzes stattfinden, und die unbehaarte apikale Tastfläche besitzen eine weitgehend voneinander unabhängige arterielle Blutzufuhr. Zwischen den Arterien und den Venen des untersuchten Gebietes existieren sehr enge nachbarliche Beziehungen; deren mögliche Bedeutung für die Blutversorgung des Schwanzes wird diskutiert.
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  • 8
    ISSN: 1432-0568
    Keywords: Ovine testis ; Postnatal development ; Actin ; Vimentin ; Tubulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The distribution pattern of actin, desmin, vimentin and tubulin in the ovine testis during postnatal development was investigated by means of immunohistochemical methods. The postnatal development of the ovine testis can be divided into five phases. Phases I through III represent the prepubertal period, phase IV puberty and phase V the postpubertal adult stage. In peritubular cells α-smooth muscle actin is present, its amount increasing with advancing age of the animals. Structural F-actin is localized in peritubular myoid cells and Sertoli cells, of the adult testis. In Sertoli cells structural F-actin-positive material is observed at the level of the Sertoli-Sertoli junctions, at contact sites of Sertoli cells with primary spermatocytes and in the immediate vicinity of elongating spermatid heads during the aerosome phase of spermiogenesis. Desmin is present in intertubular and peritubular cells during the early prepubertal period, but vanishes completely as soon as the animals reach puberty. Vimentin is present in the cytoplasm of prespermatogonia I, but disappears when these change into prespermatogonia II. In prepubertal supporting cells the vimentin content increases, and in the adult the positive filament bundles create a flame-like pattern around the unstained nucleus. Cyclical variations during the seminiferous epithelial cycle are not observed. Expression of α-tubulin is found in the cytoplasm of prespermatogonia I and to a lesser extent in prespermatogonia II and spermatogonia. The immunoreaction is also seen in the microtubules of the axonema and manchette of elongating spermatids. The histochemical demonstration of the high α-tubulin concentration in supporting and Sertoli cells is an excellent method for studying changes of cellular shape and size during ontogenesis as well as during the seminiferous epithelial cycle.
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  • 9
    ISSN: 1432-0568
    Keywords: Key words Rete testis ; Tubuli recti ; Testis development ; Mediastinum testis ; Bovine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The development of the intragonadal rete testis and the establishment of the connection between seminiferous and straight testicular tubules was studied using ultrastructural and histochemical methods in 60 bovine embryos and fetuses ranging from day 39 through day 225 post conceptionem. The methodology included a modified acetylcholinesterase (AChE) reaction as a selective marker for pre-Sertoli cells and a modified microsomal aminopeptidase (MAP) reaction as a selective marker for the epithelia of rete testis and straight testicular tubules. Between 40 and 45 days, the rete testis is predominantly an extratesticular rete situated in the cranial peduncle of the gonadal fold and in broad contact with the pro/mesonephric giant corpuscle. During this period, the intragonadal rete enters the gonad proper from its craniodorsal pole and extends into the cranial fourth of the testis. Between 60 and 110 days the rete testis attains its definitive position, extending into the central longitudinal axis as far as to the caudal fourth of the testis. For the caudal expansion of the rete testis the preceding proliferation of the mediastinal stroma is an important prerequisite. In the 40 to 45-day-old embryo the area of the testicular cords may be divided into two zones. A narrow outer zone contains plate-like cords with a thick diameter, and a larger central zone is filled with a network of thinner cords. Only the thick outer cords transform into the permanent seminiferous tubules, whereas the thinner cords in the central zone are transitory structures that disappear between 45 and 110 days. One important function of these transitory cords is to establish a continuous system of basal laminae that allows a direct connection between the central ends of the growing seminiferous tubules and the peripheral extensions of the rete testis (future straight testicular tubules). The first true straight testicular tubules become visible between 85 and 110 days. Due to a strong proliferation of the tubulus rectus-cells the straight testicular tubules elongate continuously, and the border between the rete system and the seminiferous tubules is slowly shifted towards the testicular periphery. This shift is not restricted to the prenatal period, but proceeds until after birth. At the cytological level, the formation and elongation of the straight testicular tubules is effected by proliferating cells that advance along the continuous basal lamina into the area of the seminiferous tubules. The pre-Sertoli and germ cells in this zone of invasion are separated from each other and overgrown by the tubulus rectus-cells. Exposed to the special milieu of the straight testicular tubules, pre-Sertoli and germ cells apparently cannot survive and finally disappear.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Cowpersche Drüse und Prostata produzieren bei der Ziege sowohl eine Mucosubstanz als auch ein Sekret von Eiweißcharakter. Hierbei zeigt die Cowpersdhe Drβe eine etwas stärkere Tendenz zur Mucinbildung als die Prostata. Das sehr ähnliche histochemische Enzymmuster in den sekretorisch aktiven Zellen beider Drüsen erlaubt die Schlußfolgerung, daß bei der Ziege keine so ausgeprägte Spezialisierung der kranialen (Prostata) und der kaudalen (Cowpersche Driise) Abschnitte des periurethralen Drüsenlagers eingetreten ist, wie sie bei anderen Säugetieren (z. B. Feliden, Schweine, Nager, Primaten) beobachtet wird.〈section xml:id="abs1-2"〉〈title type="main"〉SummaryHistochemical examinations of the prostatic parenchyma in the goatThe secretion of the bulbourethral and prostate glands of the goat consists of both mucous and protein substances, with the bulbourethral gland producing more mucin than the prostate. The histochemical appearance of the secretory cells in both glands is similar. It is suggested that, in the goat, a specialization of the periurethral glands into cranial (prostate) and caudal (bulbourethral) divisions is not as marked as in other mammals e. g., in the cats, pigs, rodents, and primates.〈section xml:id="abs1-3"〉〈title type="main"〉RésuméExamens histochimiques du parenchyme de la prostate chez la chèvreLa glande de Cowper et la prostate de la chèvre produisent une mucosub-stance, ainsi qu'une sécrétion de caractère protéique. La glande de Cowper manifeste une plus forte tendace à la formation de mucine que la prostate. Le schéma enzymatique histochimique trés semblable dans les cellules sécrétrices actives des deux glandes nous permet de conclure qu'il ne s'est pas produit, chez la chèvre, de différenciation très prononcée des parties craniales (prostate) et caudales (glande de Cowper) de la zone glandulaire périurétrale, telle qu'on l'observe chez d'autres mammifères (par ex. les félidées, les porcs, les rongeurs, les primates).〈section xml:id="abs1-4"〉〈title type="main"〉ResumenEstudios histoquimicos en el parénquima prostático del macho cabríoLa glándula de Cowper y la próstata producen en el macho cabrïo tanto una substancia mucosa como una secreciön con carácter proteico. La glándula de Cowper presenta una tendencia algo mayor a la formación de mucina que la próstata. El modelo histoquímico de enzimas tan parecido en las células secretoras activas de ambas glándulas permite llegar a la conclusión de que en el macho cabrío no se ha iniciado una especialización tan marcada de los tramos craneales (próstata) y de los caudales (glándula de Cowper) del lecho glandular periuretral, como se observa en otros mamíferos (v. gr. félidos, porcinos, roedores, primates).
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