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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 30 (1990), S. 220-222 
    ISSN: 1612-1112
    Keywords: Chromatography ; Quantitative analysis ; Resolution of overlapped peaks ; Exponentially-modified Gaussian function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Based on the exponentially modified Gaussian (EMG) function, a new approach is presented for the quantitation of a partially overlapped peak pair. This approach can be employed in a wide range of peak area ratio and asymmetry, provided that the relative valley between the two peaks is not greater than 55%. The first peak area is calculated by the use of the ratio of the front half-width at height fraction 0.1 to that at heigt fraction 0.5. The second peak area is calculated by subtracing the first peak area from the total area which is provided by a microprocessor-based integrator. The relative error for the first peak never exceeds ±5%, but this is not always the case for the second peak. The relative error for the second peak depends not only on the accuracy of the total area and that of the first peak area but on area ratio as well.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 32 (1991), S. 373-378 
    ISSN: 1612-1112
    Keywords: Chromatography ; Quantitative analysis ; Resolution of overlapped peak pairs ; Exponentially-modified Gaussian function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Based on the ratio of two apparent heights and an empirical correction factor, a method is presented for quantitation of peak areas of severely overlapped pairs. This method can be applied to a wide range of area ratios and peak asymmetries, provided there exists a clear and precise valley-except for shoulder peaks. The relative errors of the first peak are usually within ±3% and theoretical relative error limits are −7.0% to +5.5%. Peak asymmetry of a severely overlapped pair can be determined using the ratio of the front half-width to the rear half-width at 10% peak height of an overlapping profile. The asymmetry so determined is an apparent one and the relative errors are −4% to +5.3% for peaks with 90% relative valley, depending on area ratio, degree of overlap and asymmetry. An empirical area equation for the first peak involving area ratio, asymmetry, resolution and the area measured by a perpendicular drop algorithm is also developed.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 30 (1990), S. 400-404 
    ISSN: 1612-1112
    Keywords: Chromatography general ; Resolution function ; Skewed peaks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The widely used resolution criterion which is based on the individual base-line widths of two peaks is invalid in practical situations. In this paper, a simple resolution function is presented. All the necessary data for calculating the resolution are directly measurable from the experimental chromatogram. It is shown that this function can be used to characterize the resolution in non-ideal situations where the areas of the two peaks that overlap are different and/or the peaks are non-symmetrical.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 33 (1992), S. 397-397 
    ISSN: 1612-1112
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 35 (1993), S. 471-471 
    ISSN: 1612-1112
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 28 (1989), S. 415-416 
    ISSN: 1612-1112
    Keywords: Chromatography ; EMG peak ; Peak asymmetry ; Peak width at half height
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The criterion for an exponentially modified Gaussian (EMG) peak presented is based on the rule of the peak width for an EMG peak. An EMG peak can be judged by the agreement between the value of the peak width calculated from this rule and that measured from a real chromatographic peak.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 31 (1991), S. 595-599 
    ISSN: 1612-1112
    Keywords: Chromatography general ; Overlapping peaks ; Statistical moment analysis ; Deconvolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary By using a parameter determined from the leading half of the peak to describe the peak asymmetry, a method for the calculation of statistical moments of overlapping peaks is developed. This approach is also applicable to the deconvolution of partially overlapping peaks. The advantage of this method over the traditional summation method for the statistical moment analysis of overlapping peaks is significant.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 29 (1990), S. 248-250 
    ISSN: 1612-1112
    Keywords: General chromatographic theory ; Exponentially modified Gaussian peaks ; Gamma peaks ; Peak formation mechanisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary It is shown that part of non-EMG peaks are gamma peaks. The describing function of a gamma peak is derived. The peak area equation and the formation mechanism of a gamma peak are discussed.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 34 (1992), S. 450-452 
    ISSN: 1612-1112
    Keywords: Overlapped peaks in chromatography ; Exponentially modified Gaussian function ; Resolution of overlapping peaks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Relative non-overlap is proposed to serve as a resolution criterion. Based on the exponentially modified Gaussian (EMG) model and by using multivariate regression, an empirical equation of relative non-overlap has been developed. This equation can be employed in cases where there is a clear and precise valley between peaks.
    Type of Medium: Electronic Resource
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  • 10
    Publication Date: 2018-10-09
    Description: Alcohol is a widely used and abused substance. A major unresolved issue in the alcohol research field is determining which of the many alcohol target proteins identified to date is responsible for shaping each specific alcohol-related behavior. The large-conductance, calcium- and voltage-activated potassium channel (BK channel) is a conserved target of ethanol. Genetic manipulation of the highly conserved BK α channel influences alcohol-related behaviors across phylogenetically diverse species that include worm, fly, mouse, and man. A pharmacological tool that prevents alcohol’s action at a single target, like the BK channel, would complement genetic approaches in the quest to define the behavioral consequences of alcohol at each target. To identify agents that specifically modulate the action of ethanol at the BK channel, we executed a high-throughput phagemid-display screen in combination with a Caenorhabditis elegans behavioral genetics assay. This screen selected a novel nonapeptide, LS10, which moderated acute ethanol intoxication in a BK channel–humanized C. elegans strain without altering basal behavior. LS10’s action in vivo was dependent upon BK channel functional activity. Single-channel electrophysiological recordings in vitro showed that preincubation with a submicromolar concentration of LS10 restricted ethanol-induced changes in human BK α channel gating. In contrast, no substantial changes in basal human BK α channel function were observed after LS10 application. The results obtained with the LS10 peptide provide proof-of-concept evidence that a combined phagemid-display/behavioral genetics screening approach can provide novel tools for understanding the action of alcohol at the BK channel and how this, in turn, exerts influence over central nervous system function.
    Print ISSN: 0022-3565
    Electronic ISSN: 1521-0103
    Topics: Medicine
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