Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1435-1803
    Keywords: Long-chain acylcarnitines ; diltiazem ; mechanical function ; energy metabolism ; rat heart
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Long-chain acylcarnitines, such as palmitoyl-L-carnitine (PALCAR), are known to accumulate in the myocardium during ischemia. We examined whether exogenous PALCAR modifies the myocardial levels of high-energy phosphates (HEP) and free fatty acids (FFA) in the heart, and whetherd-cis-diltiazem andl-cis-diltiazem, an optical isomer having less potent Ca2+ channel blocking action thand-cis-diltiazem, attenuate the PALCAR-induced myocardial changes. Rat hearts were perfused aerobically at a constant flow according to the Langendorff's technique, while being paced electrically. PALCAR (5 μM) decreased the tissue levels of adenosine triphosphate and creatine phosphate and increased the tissue level of adenosine monophosphate, and produced mechanical dysfunction. In addition, PALCAR (5 μM) increased markedly the tissue levels of FFA, especially those of arachidonic and palmitoleic acids, and the release of creatine kinase (CK) from the myocardium. These alterations in the myocardial levels of HEP and FFA induced by PALCAR were significantly attenuated byd-cis-diltiazem (15 μM) orl-cis-diltiazem (15 μM). Both drugs also attenuated the PALCAR-induced CK release. The present study demonstrates that PALCAR modifies the tissue levels of HEP and FFA in the heart and that bothd-cis andl-cis-diltiazem protect the myocardium against the PALCAR-induced changes through mechanisms other that Ca2+ channel blocking action.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Munksgaard International Publishers
    Allergy 59 (2004), S. 0 
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Antigen-loaded eosinophils instilled intratracheally into mice were capable of migrating into local lymph nodes and localize to the T cell-rich paracortical zones where they stimulated antigen-specific proliferation of CD4+ T cells. The aim of the present study was to evaluate whether eosinophils within the tracheobronchial lumen can stimulate Th2 cell expansion by presenting antigen both in vitro and in vivo.Methods:  Airway eosinophils were recovered from ovalbumin-sensitized and -challenged BALB/c mice, these eosinophils were then co-cultured with sensitized CD4+ cells in the absence or presence of anti-CD80 or/and -CD86 monoclonal antibodies. Airway eosinophils were instilled into the trachea of sensitized mice. At 3 days thereafter, the draining paratracheal lymph nodes were removed and teased into cell suspensions for culture. Cell-free culture supernatants were collected for detection of cytokines.Results:  Our data showed that airway eosinophils functioned as CD80- and CD86-dependent antigen-presenting cells (APCs) to stimulate sensitized CD4+ lymphocytes to produce interleukin (IL)-4, IL-5, and IL-13, but not interferon (IFN)-γ in in vitro assay. When instilled intratracheally in sensitized recipient mice, airway eosinophils migrated into draining paratracheal lymph nodes primed Th2 cells in vivo for IL-4, IL-5, and IL-13, but not IFN-γ, production during the in vitro culture that was also CD80- and CD86-dependent.Conclusion:  Eosinophils within the lumina of airways could process inhaled antigen function in vitro and in vivo as APCs to promote expansion of Th2 cells. This investigation highlights the potential of eosinophils to not only act as terminal effector cells but also to actively modulate immune responses by amplifying Th2 cell responses.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 117 (2002), S. 2377-2388 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Brownian dynamics simulations have been carried out to model attractive polymers in solution. Bead–spring polymer chains with attractions between the end beads were modeled over a wide concentration range on either side of the overlap concentration, ρ*, for the corresponding random coil polymers. The polymers were treated as beads linked by finitely extensible nonlinear elastic springs and the excluded volume repulsion between unlinked beads was represented by a pair potential with a Gaussian analytic form. For the sticky end-beads the potential has an attractive tail of Gaussian form. In addition to chains with purely repulsive bead–bead nonbonded interactions, three different systems with attactive end beads were modeled. There were those with (a) head–head (H–H) attractions, (b) with both H–H and tail–tail (T–T) attractions, and (c) with head–tail (H–T) attractions. The dimensions of the chains, the bead–bead radial distribution functions, as well as the dynamic properties such as stress tensor time-correlation functions, infinity frequency elastic modulus, and specific viscosity of the solution were calculated as functions of solution density and end-bead attraction class. We show that with the three classes of attractive end-bead functionality, the model polymers all depart from random coil statistics and show evidence of enhanced association, even in the dilute regime, especially for the H–T systems, which can form necklacelike structures at low dilution and micellelike states with increasing concentration. (Not all of the polymer statistics measures show major differences though.) However, only the rheology if the H–T system is markedly different from the random coil case. The rheology of the H–T system is quite different in qualitative and quantitative behavior to the other classes studied. There is a progressive retardation and increasingly near-exponential decay in the shear stress relaxation function. The viscosity of the H–T class of polymers is typically at least an order of magnitude higher than that of the others, even at concentrations far below the overlap concentration ρ* for such polymers. The infinite frequency elastic modulus is also typically about five times larger for the H–T class across the density range when compared with the other three types modeled. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 111 (1999), S. 10694-10705 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Brownian dynamics simulations have been carried out for model polymer chains in a good solvent over a wide concentration range. The polymers were treated as beads linked by finitely extensible nonlinear elastic (FENE) springs and the repulsion between any two unlinked beads was modeled by a pair potential with a Gaussian analytic form, βu(r)=A exp(−r2/σ2), where β=1/kT, A and σ are characteristic energy and distance scales, respectively. The effects of the bead–bead repulsion on the structure and time-correlation functions of the chains in the polymer solution were studied as a function of polymer concentration. Three concentration regimes are distinguished, a dilute region where intrachain bead–bead repulsions dominate, a concentrated region where interchain bead–bead repulsions dominate, and a highly concentrated region where the net repulsion on any bead tends to zero owing to substantial cancellation of the effects from nearest neighbors. The pair radial distribution function, the relaxation time for the rotation of the coil, the mean square displacement of the middle-bead and that of the center of mass of the chain, the infinity frequency elastic modulus, and the viscosity of the system are examined in all the density regions. Our results show that the excluded volume repulsion strongly affects the behavior of the system in the concentrated region and that the structural features return to the Rouse-limit behavior at high density more rapidly than the dynamical properties. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    New York, NY : American Institute of Physics (AIP)
    Physics of Fluids 5 (1993), S. 3961-3968 
    ISSN: 1089-7666
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Application of a short current pulse on a nominal Ohmic discharge in the STOR-M tokamak (Saskatchewan Torus-Modified) [Phys. Fluids B 4, 3277 (1992)] triggers the Ohmic H-mode characterized by reduced Hα radiation, increased electron density, and reduced edge density/magnetic fluctuations. Measurements of plasma floating potential at the plasma edge and in the scrape-off layer indicate that the Ohmic H-mode is accompanied by negative plasma autobiasing, which leads to a steeper radial electric field profile at the edge. Since the duration of the current pulse (≤20 kA, 100 μsec) is shorter than the resistive skin time ((approximately-equal-to)1 msec), preferential edge heating is expected, which is believed to be responsible for changes in the edge discharge condition favorable for inducing the Ohmic H-mode. The electron density profile becomes steeper at the edge during the H-mode, and clear formation of a density pedestal has been seen. The evolution of the density profile suggests the presence of particle pinch. An improved confinement phase (ICP) is induced by external negative electrode biasing. The ICP reveals some similarities as compared to the current pulse induced H-mode. It is found that the electrostatic modes are dominant in the scrape-off layer while electromagnetic modes dominate in the plasma edge during the normal Ohmic discharges.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Polymorphism of HLA-DPB1 was revealed with a sequencing-based typing (SBT) method in 47 unrelated healthy individuals from Yunnan Hani ethnic minority. The alleles DPB1*5901 and DPB1*7001 were detected for the first time in Chinese populations. A dendrogram showed that the Hani ethnic group belongs to the southern group of Chinese.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 8
    ISSN: 1432-2242
    Keywords: Zea mays L. ; Pollen ; mRNAs ; Protein synthesis ; Germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mature ungerminated pollen grains of Zea mays L. contain presynthesized messenger RNAs. This has been demonstrated by the isolation of poly(A)RNA and its translation in the wheat germ and reticulocyte cell free systems into polypeptides many of which are similar to those synthesized in germinating pollen. Each corn pollen grain contains between 352–705 pg of total RNA and 8.9–17.8 pg of poly(A)RNA. During germination of corn pollen at least 260 different polypeptides are synthesized as determined by labeling and 2-dimensional gel electrophoresis. These results are discussed with reference to other plants and the number of different genes expressed during pollen development.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 9
    ISSN: 1435-1803
    Keywords: Key words Long-chain acylcarnitines – diltiazem – mechanical function – energy metabolism – rat heart
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Long-chain acylcarnitines, such as palmitoyl-L-carnitine (PALCAR), are known to accumulate in the myocardium during ischemia. We examined whether exogenous PALCAR modifies the myocardial levels of high-energy phosphates (HEP) and free fatty acids (FFA) in the heart, and wether d-cis-diltiazem and l-cis-diltiazem, an optical isomer having less potent Ca2+ channel blocking action than d-cis-diltiazem , attenuate the PALCAR-induced myocardial changes. Rat hearts were perfused aerobically at a constant flow according to the Langendorff's technique, while being paced electrically. PALCAR (5 μM) decreased the tissue levels of adenosine triphosphate and creatine phosphate and increased the tissue level of adenosine monophosphate, and produced mechanical dysfunction. In addition, PALCAR (5 μM) increased markedly the tissue levels of FFA, especially those of arachidonic and palmitoleic acids, and the release of creatine kinase (CK) from the myocardium. These alterations in the myocardial levels of HEP and FFA induced by PALCAR were significantly attenuated by d-cis-diltiazem (15 μM). Both drugs also attenuated the PALCAR-induced CK release. The present study demonstrates that PALCAR modifies the tissue levels of HEP and FFA in the heart and that both d-cis- and l-cis-diltiazem protect the myocardium against the PALCAR indueced changes through mechanisms other than Ca2+ channel blocking action.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 76 (2000), S. 3112-3114 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Bi-based layered ferroelectric thin films of Sr2Bi4Ti5O18 (SBTi) were prepared by pulsed-laser deposition. The c-axis-oriented SBTi films were grown on SrRuO3 seeded Pt/TiO2/SiO2/Si substrates while polycrystalline SBTi films were grown on Pt/TiO2/SiO2/Si substrates. The measurements of ferroelectricity revealed that the direction of spontaneous polarization was not along the c axis since the polarization of c-axis-oriented films was much less than that of randomly oriented films. There was no significant degradation of switchable charge at least up to 1011 cycles for the randomly oriented films, suggesting that, even with Ti which was widely accepted to contribute to the fatigue of Pb(Zr, Ti)O3, SBTi showed superior fatigue characteristics. The randomly oriented films also showed excellent retention characteristics after 105 s. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...