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  • 1
    New York, NY : Springer
    Keywords: Medical laboratories ; Microbiology ; Biochemistry ; Laboratory Medicine ; Medical Microbiology ; Biochemistry, general ; Springer eBooks
    Description / Table of Contents: Sustainability in Biobanking- An Introduction to Starting a Biobank -- An Introduction to Hardware, Software, and Other Information Technology Needs of Biomedical Biobanks -- Disaster Prevention and Recovery -- Minority Participation in Biobanks: An Essential Key to Progress -- Orientation and Training of New Biobank Personnel -- Procurement and Storage of Surgical Biospecimens -- Autopsy Biobanking: Biospecimen Procurement, Integrity, Storage, and Utilization -- Procurement, Storage, and Use of Blood in Biobanks -- Procurement, Transportation, and Storage of Saliva, Buccal Swab, and Oral Wash Specimens -- Biobanking of Cerebrospinal Fluid -- Biobanking of Urine Samples -- Procurement and Storage of Pleural and Peritoneal Fluids for Biobanking -- The Fluid Preservation of Zoological Specimens -- Photographing Fluid Preserved Specimens -- Casts of Fluid Preserved Specimens -- Collection and Preservation of Terrestrial Arthropods -- Procurement and Preservation of Plants -- Biosafety and Biohazards: Understanding Biosafety Levels and Meeting Safety Requirements of a Biobank -- Decontamination of Biobank Facilities -- Laboratory Safety: Chemical and Physical Hazards -- Making Formalin-Fixed Paraffin Embedded Blocks -- Microtomy: Cutting Formalin-Fixed Paraffin Embedded Sections -- Performing and Cutting Frozen Sections -- An Introduction to the Performance of Immunohistochemistry -- An Introduction to Performing Immunofluorescence Staining -- Making a Tissue Microarray -- Nucleic Acid Isolation and Quality Control -- Fundamentals of RNA Analysis on Biobanked Specimens -- Nucleic Acid Extraction from Human Biological Samples -- An Overview of DNA Analytical Methods -- Shotgun Proteomic Profiling of Blood Borne Nanoscale Extracellular Vesicles (NsEVs) -- Sample Preparation for Transmission Electron Microscopy -- Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines -- Domestic and International Shipping of Biospecimens
    Abstract: This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and comprehensive, Biobanking: Methods and Protocols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies
    Pages: XVI, 448 p. 66 illus., 49 illus. in color. : online resource.
    ISBN: 9781493989355
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  • 2
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Adrenocorticotrophic hormone (ACTH)-secreting pituitary tumors are associated with high morbidity due to excess glucocorticoid production. No suitable drug therapies are currently available, and surgical excision is not invariably curative. Here we demonstrate immunoreactive expression of the ...
    Type of Medium: Electronic Resource
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