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  • 1
    Keywords: IN-VITRO ; human ; IN-VIVO ; LUNG ; VITRO ; VIVO ; DNA adducts ; liver ; NEW-YORK ; GENE ; TISSUE ; MICE ; ACTIVATION ; DNA ; kidney ; 3-nitrobenzanthrone ; DIESEL EXHAUST ; HETEROCYCLIC AMINES ; INDUCTION ; RAT ; BODY-WEIGHT ; CONTAMINANT 3-NITROBENZANTHRONE ; RATS ; TISSUES ; BINDING ; SEQUENCE ; treatment ; FREQUENCY ; METABOLITES ; MOUSE ; MUTANT ; TRANSGENIC MICE ; PATTERNS ; ASSAY ; MUTATION ; BLADDER ; DNA-BINDING ; NUCLEOTIDES ; POLLUTANT 3-NITROBENZANTHRONE ; MUTATIONS ; ADDUCTS ; TESTIS ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; 3-nitrobenzanthrone,Muta Mouse,mutation spectra,cll,DNA adducts,P-32-post-labeling,diesel exhaust,ai ; CII GENE ; DEOXYADENOSINE ; DNA-ADDUCTS ; LAMBDA/LACZ TRANSGENIC MICE ; micronuclei ; POTENT ; SURFACE SOIL ; V79 CELLS
    Abstract: 3-nitrobenzanthrone (3-NBA) is an extremely potent mutagen in the Salmonella reversion assay and a suspected human carcinogen identified in diesel exhaust and in ambient airborne particulate matter. To evaluate the in vivo mutagenicity of 3-NBA, we analyzed the mutant frequency (MF) in the cll gene of various organs (lung, liver, kidney, bladder, colon, spleen, and testis) in lambda/lacZ transgenic mice (Muta Mouse) after intraperitoneal treatment with 3-NBA (25 mg/kg body weight injected once a week for 4 weeks). Increases in MF were found in colon, liver, and bladder, with 7.0-, 4.8-, and 4.1-fold increases above the control value, respectively, whereas no increase in MF was found in lung, kidney, spleen, and testis. Simultaneously, induction of micronuclei in peripheral blood reticulocytes was observed. The sequence alterations in the cll gene recovered from 41 liver mutants from 3-NBA-treated mice were compared with 32 spontaneous mutants from untreated mice. Base substitution mutations predominated for both the 3-NBA-treated (80%) and the untreated (81%) groups. However, the proportion of G:C--〉T:A transversions in the mutants from 3-NBA-treated mice was higher (49% vs. 6%) and the proportion of G:C--〉A:T transitions was lower than those from untreated mice (10% vs. 66%). The increase in MF in the liver was associated with strong DNA binding by 3-NBA, whereas in lung, in which there was no increase in MF, a low level of DNA binding was observed (268.0-282.7 vs. 8.8-15.9 adducts per 10(8) nucleotides). DNA adduct patterns with multiple adduct spots, qualitatively similar to those formed in vitro after activation of 3-NBA with nitroreductases and in vivo in rats, were observed in all tissues examined. Using high-pressure liquid cochromatographic analysis, we confirmed that all major 3-NBA-DNA adducts produced in vivo in mice are derived from reductive metabolites bound to purine bases (70-80% with deoxyguanosine and 20-30% with deoxyadenosine in liver). These results suggest that G:C--〉T:A transversions induced by 3-NBA are caused by misreplication of adducted guanine residues through incorporation of adenine opposite the adduct (A-rule)
    Type of Publication: Journal article published
    PubMed ID: 15065206
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Optics and Lasers in Engineering 16 (1992), S. 279-288+291 
    ISSN: 0143-8166
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Electrical Engineering, Measurement and Control Technology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics , Technology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Optics Communications 82 (1991), S. 229-235 
    ISSN: 0030-4018
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 60 (1989), S. 3195-3200 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: Fiber-optic laser-Doppler anemometry (LDA) probes based on the use of multimode, graded-index fibers are demonstrated. Conditions for fundamental transmission of laser light in these fibers are discussed, and an input coupling based on the use of SELFOC rod lenses is presented. The concept is demonstrated on several individual fiber links and on a two-component, backscatter LDA probe. The main advantages of this system lie in the simplicity and robustness of the input coupling mechanics while maintaining high transmitted power levels.
    Type of Medium: Electronic Resource
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  • 5
    Publication Date: 2018-10-17
    Description: Alternative pre-mRNA splicing remarkably expands protein diversity in eukaryotes. Drosophila PGRP-LC can generate three major 3' splice isoforms that exhibit distinct innate immune recognition and defenses against various microbial infections. However, the regulatory mechanisms underlying the uniquely biased splicing pattern at the 3' variable region remain unclear. Here we show that competing RNA pairings control the unique splicing of the 3' variable region of Drosophila PGRP-LC pre-mRNA. We reveal three roles by which these RNA pairings jointly regulate the 3' variant selection through activating the proximal 3' splice site and concurrently masking the intron-proximal 5' splice site, in combination with physical competition of RNA pairing. We also reveal that competing RNA pairings regulate alternative splicing of the highly complex 3' variable regions of Drosophila CG42235 and Pip . Our findings will facilitate a better understanding of the regulatory mechanisms of highly complex alternative splicing as well as highly variable 3' processing.
    Print ISSN: 1355-8382
    Electronic ISSN: 1469-9001
    Topics: Biology , Medicine
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  • 6
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2018-11-01
    Description: It is crucial but of a great challenge to study in vivo and in situ drug release of nanocarriers when developing a nanomaterial-based drug delivery platform. We developed a new label-free laser desorption/ionization mass spectrometry (MS) imaging strategy that enabled visualization and quantification of the in situ drug release in tissues by monitoring intrinsic MS signal intensity ratio of loaded drug over the nanocarriers. The proof of concept was demonstrated by investigating the doxorubicin (DOX)/polyethylene glycol–MoS 2 nanosheets drug delivery system in tumor mouse models. The results revealed a tissue-dependent release behavior of DOX during circulation with the highest dissociation in tumor and lowest dissociation in liver tissues. The drug-loaded MoS 2 nanocarriers are predominantly distributed in lung, spleen, and liver tissues, whereas the accumulation in the tumor was unexpectedly lower than in normal tissues. This new strategy could also be extended to other drug-carrier systems, such as carbon nanotubes and black phosphorus nanosheets, and opened a new path to evaluate the drug release of nanocarriers in the suborgan level.
    Electronic ISSN: 2375-2548
    Topics: Natural Sciences in General
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  • 7
    Publication Date: 2018-11-09
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Geosciences , Computer Science , Medicine , Natural Sciences in General , Physics
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