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  • 1
    Keywords: SYSTEM ; SITES ; PROTEIN ; PROTEINS ; MECHANISM ; IONS ; PHOSPHORYLATION ; ACID ; PERFORMANCE ; IDENTIFICATION ; tandem mass spectrometry ; MASS-SPECTROMETRY ; METHYLTRANSFERASE ; MS ; LC-MS ; PHOSPHORYLATED PEPTIDES ; additive ; reverse phase ; IMAC ; highly phosphorylated phosphopeptides ; PHASES ; SETDB1
    Abstract: Incomplete recovery from the LC column is identified as a major cause for poor detection efficiency of phosphopeptides by LC-MS/MS. It is proposed that metal ions adsorbed on the stationary phase interact with the phosphate group of phosphopeptides via an ion-pairing mechanism related to IMAC (IMAC: immobilized metal ion affinity chromatography). This may result in their partial or even complete retention. Addition of phosphate, EDTA or citrate to the phosphopeptide sample was tested to overcome the detrimental phosphopeptide suppression during gradient LC-MS/MS analysis, while the standard solvent composition (water, acetonitrile, formic acid) of the LC system was left unchanged. With the use of UPLC, a citrate additive was found to be highly effective in increasing the phosphopeptide detection sensitivity. Addition of EDTA was found to be comparable with respect to sensitivity enhancement, but led to fast clogging and destruction of the spray needle and analytical columns due to precipitation. In contrast, a citrate additive is compatible with prolonged and stable routine operation. A 50 mM citrate additive was tested successfully for UPLC-MS analysis of a commercial four-component phosphopeptide mixture, a tryptic beta-casein digest, and several digests of the 140 kDa protein SETDB1. In this protein, 27 phosphorylation sites could be identified by UPLC-MS/MS using addition of citrate, including the detection of several phosphopeptides carrying 3-5 pSer/pThr residues, compared to identification of only 10 sites without citrate addition. A 50 mM citrate additive particularly increases the recovery of multiply phosphorylated peptides, thus, extending the scope of phosphopeptide analysis by LC-MS/MS
    Type of Publication: Journal article published
    PubMed ID: 19053530
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  • 2
    ISSN: 0888-7543
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0888-7543
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0888-7543
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ataxia-telangiectasia (A-T) is a progressive autosomal recessive disease featuring neurodegeneration, immunodeficiency, chromosomal instability, radiation sensitivity and a highly increased proneness to cancer. A-T is ethnically widespread and genetically heterogeneous, as indicated by the existence of four complementation groups in this disease. Several “A-T-like” genetic diseases share various clinical and cellular characteristics with A-T. By using linkage analysis to study North American and Turkish A-O families, the ATA (A-T, complementation group A) gene has been mapped to chromosome 11q23. A number of Israeli Arab A-T patients coming from large, highly inbred families were assigned to group A In one of these families, an additional autosomal recessive disease was identified, characterized by ataxia, hypotonia, microcephaly and bilateral congenital cataracts. In two patients with this syndrome, normal levels of serum immunoglobulins and alpha-fetoprotein, chromosomal stability in peripheral blood lymphocytes and skin fibroblasts, and normal cellular response to treatments with X-rays and the radiomimetic drug neocarzinostatin indicated that this disease does not share, with A-T, any additional features other than ataxia. These tests also showed that another patient in this family, who is also mentally retarded, is affected with both disorders. This conclusion was further supported by linkage analysis with 11q23 markers. Lod scores between A-O and these markers, cumulated over three large Arab families, were significant and confirmed the localization of the ATA gene to aq23. However, another Druze family unassigned to a specific complementation group, showed several recombinants between A-T and the same markers, leaving the localization of the A-T gene in this family open.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1435-0130
    Keywords: Key words 3-D CT ; Breast reconstruction ; Implant volume
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Experience in the estimation of breast implant volume for reconstruction is presented. Three-dimensional computed tomography (CT) was used in a group of 22 patients having this examination as part of another study. The volume of the final implant was estimated by measuring the intact breast volume. The final clinical results showed excellent volume symmetry.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-1262
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé La multiplication des registres de polypose a permis de diagnostiquer un nombre croissant de sujets jeunes proteurs d'une polypose adénomateuse familiale (FAP). Afin d'établir des recommandations dans la sélection du traitement chirurgical approprié chez des adolescents (10–19 ans), nous avons comparé les résultats de la colectomie et de l'anastomose iliéo-rectale (IRA, n=17 patients) aux résultats de procto-colectomie avec rétablissement de la continuité par l'intermédiaire d'une poche iléoanale (IPAA, n=7 patients). Les dossiers ont été revus afin d'obtenir des données sur la technique opératoire, les pertes sanguines et les transfusions, la durée de séjours hospitaliers (incluant la période nécessaire pour la fermeture de l'iléostomie), ainsi que les complications précoces (à moins de 30 jours de l'opération) et les complications tardives. Les résultats fonctionnels (nombre de selles par 24 h, usage de médications anti-diarrhéïques, fuites et incontinence fécale) et la qualité de la vie ont été évalués prospectivement à l'aide d'un questionnaire et de consultations de contrôle. Le suivi moyen a été de 49 mois (de 6 à 95 mois) après l'IRA et de 36 mois après l'IPAA (de 4 à 87 mois). Bien que la colo-poctectomie avec rétablissement de la continuité par IPAA soit plus longue (5,75-heures vs 3,1) plus sanglante (500 ml pertes sanguines vs 300 ml) et plus complexe avec un séjour hospitalier plus long (12 jours vs 7 jours) que l'IRA (P=0.008, P=0.006 et P=0.002), nous n'avons pas observé de différence P〉0.05 entre les deux groupes en ce qui concerne le taux de complecations et la qualité de vie. Nous recommandons une colo-proctectomie avec rétablissement de la continuité par une IPAA chez des adolescents porteurs d'une polypose adénomateuse familiale et de tapis d'adénome du rectum présentant des cancers curables des deux tiers supérieurs du rectum chez lesquels le follow-up ne pourra que difficilement être assuré. Dans les autres cas, la décision de réaliser une IRA ou une procto-colectomie avec IPAA dépend des souhaits du patient et de l'expérience du chirurgien.
    Notes: Abstract Increasing numbers of polyposis registries have led to more young patients being diagnosed with familial adenomatous polyposis (FAP). To provide guidelines for selecting the appropriate surgical procedure in teenagers (10–19 years), we compared the results of colectomy and ileo-rectal anastomosis (IRA, n=17 patients) to the results of resrorative proctocolectomy and ileal pouch-anal anastomosis (IPAA, n=7 patients). Charts were reviewed to obtain data on the operative technique, blood loss and transfusions, hospital stay (including the time for ileostomy closure), and early (within 30 days of surgery) and late complications. Functional results (bowel movements per 24h, use of antidiarrheal drugs, seepage, and fecal incontinence) and quality of life were evaluated prospectively with a questionnaire and physical examination. The median follow-up time was 49 months (range, 6 to 95 months) after IRA, and 36 months after IPAA (range, 4 to 87 months). Although restorative proctocolectomy and IPAA, is a longer (5.75 vs 3.1 hours), more bloody (500 vs 300 mL blood loss), and more complex operation with a longer hospital stay (12 vs 7 days) than IRA (P=0.008, P=0.006, P=0.02, respectively), no significant difference (P〉0.05) was found between groups concerning the complication rate or quality of life. For teenagers with FAP and rectal carpeting, large rectal adenomas, curable cancer in the upper two-thirds of the rectum, or who are unavailable for follow-up, we recommend a restorative proctocolectomy and IPAA. For the other patients, the decision whether to perform IRA or restorative proctocolectomy with IPAA depends on the patient's desire and the surgeon's skill.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1262
    Keywords: Key words Diverticulitis ; Age ; Location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  To examine the effect of patient’s age and the location of diverticular disease on the course of the acute disease we retrospectively collected demographic data, symptoms, laboratory findings, imaging techniques, type of treatment (conservative vs. surgical), early and late complications, and follow-up data on 119 patients with acute diverticulitis (74 women, 45 men; mean age 64±14 years; follow-up 7–102 months, median 40). Patients were divided by their age into two groups (42 aged 60 years or younger, 77 aged over 60) and on the location of their disease (108 to the left of the middle transverse, 11 to the right). Lower abdominal pain, abdominal tenderness, and fever were the most common complaints (70–97%). In the younger patients we found a significantly greater preponderance in the right colon (P=0.02) than in older patients. Abdominal abscesses and fistulas were more common in right-sided diverticulitis (P=0.01). Patients with right-sided colon diverticulitis were treated surgically (82%) and on an emergency basis more often than patients with left-side colon diverticulitis (25%; P=0.001). Older patients treated conservatively suffered more than younger patients (61% and 33% respectively; P=0.04) from recurrent abdominal pain but not from recurrent, confirmed diverticulitis. Patients with right-sided diverticulitis treated conservatively suffered more from recurrent diverticulitis episodes than patients with left-sided diverticulitis (P=0.05). Younger patients thus do not have a more aggressive form of diverticulitis than older patients. Patients with acute diverticulitis in the right colon are likely to be operated earlier and for mistaken diagnoses than patients with left-sided diverticulitis.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1530-0358
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1530-0358
    Keywords: Hemorrhage ; Rectovaginal fistula ; Embolization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract PURPOSE: Patients rarely have intractable hemorrhage from rectovaginal fistulas, which usually require surgical intervention. This report presents our experience with nonsurgical treatment of a high-risk patient with uncontrolled hemorrhage originating from a malignant rectovaginal fistula. METHODS: A 74-year-old female developed uncontrolled hemorrhage from a malignant rectovaginal fistula. Because of her poor physical condition, an embolization with metal clips of the right and left hypogastric arteries was performed, distal to the superior gluteal artery. RESULTS: Embolization was successful in controlling the rectovaginal bleeding, allowing the patient to live 12 months. She refused adjuvant radiotherapy or chemotherapy. CONCLUSIONS: Selective angiography and embolization is a worthwhile alternative in patients with uncontrolled bleeding from a malignant rectovaginal fistula who are poor candidates for surgical intervention.
    Type of Medium: Electronic Resource
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