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  • 1980-1984  (3)
  • 1955-1959
  • 1925-1929
  • 1984  (3)
Collection
Publisher
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  • 1980-1984  (3)
  • 1955-1959
  • 1925-1929
Year
  • 1
    ISSN: 1432-2307
    Keywords: Carcinoid ; Immunocytochemistry ; Cytokeratin ; Neuron specific enolase ; Histogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Occurrence and distribution of cytokeratin, neuron specific enolase (NSE) and actin were studied by the immunoperoxidase-antiperoxidase (PAP)-technique using specific antibodies in formalin-fixed, paraffin-embedded material from 6 cases of neurogenic appendicopathy with numerous endocrine cells in the mucosal stroma (SEC), 5 cases of microcarcinoidosis of the stomach, 12 gastrointestinal carcinoids and 4 bronchial carcinoids. Cytokeratin was detectable in all tumor cells. In addition, the epithelial endocrine cells (EEC) and the SEC of intestinal origin were cytokeratin positive. EEC, SEC and cells of microcarcinoids and carcinoids showed a positive immunoreactivity with antibodies to NSE, whereas actin antibodies did not reveal significant staining of these cells. These results strongly suggest that carcinoids of the gastrointestinal tract originate from SEC that have migrated downwards into the stroma from the epithelial layer (“Endophytie” according to Feyrter).
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2307
    Keywords: Immunohistochemistry ; Pituitary gland ; Pituitary adenomas ; Cytokeratins ; Intermediate filaments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ten non-neoplastic pituitary glands and 22 pituitary adenomas producing different hormones were studied by immunofluorescence microscopy as well as peroxidase-antiperoxidase and biotin-avidin techniques on frozen sections and formalin-fixed, paraffin-embedded material using antibodies to cytokeratin, vimentin, GFAP, neurofilament protein and different pituitary hormones. The endocrine cells in non-neoplastic pituitary glands as well as in most pituitary adenomas were cytokeratin-positive. The cytoplasmic cytokeratin distribution patterns of non-neoplastic and tumor cells were similar and typical of the type of hormone produced: GH-producing normal cells showed a paranuclear condensation of cytokeratin-reactive intermediate filaments; this accumulation was even further accentuated in GH-producing adenomas resulting in fibrous bodies (Kovacs and Horvath 1978) decorated by cytokeratin antibodies. Prolactin-producing cells showed a less intense cytoplasmic cytokeratin-specific staining with focal paranuclear accentuation in non-neoplastic as well as in neoplastic glands. ACTH-producing cells in normal pituitary glands as well as in adenomas exhibited a strong and more uniform cytoplasmic cytokeratin staining. The cytokeratin reactivity in glycoprotein hormone-producing cells of non-neoplastic tissue and adenomas was weak. Vimentin and GFAP reactivity was confined to agranular folliculo-stellate cells. The specific and different distribution patterns of cytokeratins in pituitary cells can, therefore, provide an (indirect) indication to the production of a specific hormone if immunocytochemistry fails to demonstrate hormone production.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0533
    Keywords: Immunohistochemistry ; Folliculo-stellate cells ; Pituitary gland ; Pituitary adenoma ; S-100 protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Presence and distribution of S-100 protein (S-100), neuron-specific enolase (NSE), cytokeratin polypeptides, glial fibrillary acidic protein (GFAP), vimentin, actin, lysozyme and pituitary hormones (prolactin, hGH, ACTH, β-FSH, β-LH, β-TSH, alpha subunit) in folliculo-stellate cells (FSC) were studied in seven normal human pituitary glands and 28 pituitary adenomas using peroxidase-antiperoxidase and the avidin-biotin immunohistochemical techniques. Approximately 5% of the cells of the adenohypophysis were agranular, non-hormon-producing FSC most of which showed a conspicuous and strong reaction with S-100 antibodies but some were, in addition, GFAP- and vimentin-positive. In contrast to endocrine cells (EC), FSC were not decorated by antibodies to NSE or cytokeratins. In addition to supportive functions, these cells, due to their close special relationship to EC, seem to have transport and other metabolic functions yet to be elucidated. By their S-100 reactivity and their distribution FSC are comparable to glial cells of the central and schwann and satellite cells of the peripheral nervous system (PNS) as well as to supportive cells in neuroendocrine organs and related tumors (e.g., pheochromocytomas, paragangliomas, carcinoids). With one exception, S-100 reactive FSC were not found in pituitary adenomas. The immunohistochemical demonstration of S-100 protein in pituitary tissue is, therefore, a reliable aid in the discrimination between adenomas and normal pituitary tissue, particularly in small and poorly preserved specimens. In one adenoma FSC were found in addition to ACTH-producing tumor cells. This seems to be an extremely rare event suggesting a combination tumor.
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