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  • 2015-2019  (4)
  • 2018  (4)
  • 1
    Keywords: Life sciences ; Proteins ; Life sciences ; Protein Science ; Springer eBooks
    Description / Table of Contents: Protein Engineering - Past, Present, and Future -- (Semi)rational Protein Design -- Computational Analysis of Protein Tunnels and Channels -- YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations -- A Computational Library Design Protocol for Rapid Improvement of Protein Stability ́€“ FRESCO -- Directed Evolution of Proteins Based on Mutational Scanning -- A Brief Guide to the High-throughput Expression of Directed Evolution Libraries -- Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli using MTP Shakers -- Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification -- Functional Analysis of Membrane Proteins Produced by Cell-Free Translation -- Practical Considerations Regarding the Choice of the Best High-throughput Assay -- High-throughput Screening Assays for Lipolytic Enzymes -- Continuous High-throughput Colorimetric Assays for Îł-transaminases -- Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases -- Directed Co-evolution of Two Cellulosic Enzymes in Escherichia coli Based on their Synergistic Reactions -- Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation -- Solid Phase Agar Plate Assay for Screening Amine Transaminases -- Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics -- Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display -- Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophiles.℗
    Abstract: This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments.℗
    Pages: XI, 352 p. 82 illus., 63 illus. in color. : online resource.
    ISBN: 9781493973668
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  • 2
    German Medical Science GMS Publishing House; Düsseldorf
    In:  69. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Mexikanischen und Kolumbianischen Gesellschaft für Neurochirurgie; 20180603-20180606; Münster; DOCV290 /20180618/
    Publication Date: 2018-06-19
    Keywords: ddc: 610
    Language: English
    Type: conferenceObject
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  • 3
    Publication Date: 2018-01-11
    Description: AATF suppresses apoptosis, promotes proliferation and is critical for Kras -driven lung cancer AATF suppresses apoptosis, promotes proliferation and is critical for 〈i〉Kras〈/i〉-driven lung cancer, Published online: 11 January 2018; doi:10.1038/s41388-017-0054-6 AATF suppresses apoptosis, promotes proliferation and is critical for Kras -driven lung cancer
    Print ISSN: 0950-9232
    Topics: Medicine
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  • 4
    Publication Date: 2018-04-01
    Description: Sensors, Vol. 18, Pages 1052: New Digital Metal-Oxide (MOx) Sensor Platform Sensors doi: 10.3390/s18041052 Authors: Daniel Rüffer Felix Hoehne Johannes Bühler The application of metal oxide gas sensors in Internet of Things (IoT) devices and mobile platforms like wearables and mobile phones offers new opportunities for sensing applications. Metal-oxide (MOx) sensors are promising candidates for such applications, thanks to the scientific progresses achieved in recent years. For the widespread application of MOx sensors, viable commercial offerings are required. In this publication, the authors show that with the new Sensirion Gas Platform (SGP) a milestone in the commercial application of MOx technology has been reached. The architecture of the new platform and its performance in selected applications are presented.
    Electronic ISSN: 1424-8220
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Published by MDPI Publishing
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