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  • 2005-2009  (2)
  • 1965-1969  (1)
  • 1
    German Medical Science; Düsseldorf, Köln
    In:  50. Jahrestagung der Deutschen Gesellschaft für Medizinische Informatik, Biometrie und Epidemiologie (gmds), 12. Jahrestagung der Deutschen Arbeitsgemeinschaft für Epidemiologie; 20050912-20050915; Freiburg im Breisgau; DOC05gmds321 /20050908/
    Publication Date: 2005-09-09
    Keywords: ddc: 610
    Language: English
    Type: conferenceObject
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  • 2
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The aim of this study was to establish a co-culture of bovine hoof keratinocytes and fibroblasts in different types of perfusion chambers under defined conditions. The perfusion chamber PCS3c (Oligene, Berlin) was used to culture dermal fibroblasts and epidermal keratinocytes separated by a Millicell® Insert (Millipore, Schwalbach). In addition novel perfusion chambers developed by Dirk Hoffmann were used in combination with S & S membrane filters (Schleicher & Schuell, Dassel). First, fibroblasts were seeded onto one side of the insert/membrane and allowed to grow for 4 days. Subsequently keratinocytes were seeded onto the other side and allowed to adhere for one day. Then the chambers were connected to the tubing system with an attached 8-channel pump and a gas permeable media bag. We worked with different media and flow rates ranging from 0.035 ml/min to 0.35 ml/min. The system was run for up to 20 days. After a few days in culture the cells had grown to confluence. Then keratinocytes began to differentiate and built up stratified colonies. Within these colonies the cells showed the characteristic morphology of a stratified squamous epithelium. The use of perfusion chambers allows three-dimensional cultures to grow and survive for weeks, because of an unlimited medium supply and gas exchange. Additional, perfusion chamber systems enable the co-cultivation of fibroblasts and keratinocytes separated by membranes, which permit the exchange of molecules like growth factors. Furthermore, it is possible to perfuse the two different parts of the perfusion chamber with various and even different types of media. This provides the opportunity to add a growth factor to the medium for only one cell type and to study the effect of this factor to the other cell type. This work was supported by the European Communities under the Lamecow project QLK5-CT-2002-00969.The authors are solely responsible and the work does not necessarily represent the opinion of the European Communities.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Serial examinations of rabbit eyes at different times following intravenous injections of a blastospore suspension of Candida albicans were performed. All histological findings in PAS stained slides demonstrate that the microorganisms reach the eye via the ciliary arteries and infection starts primarily in the choroid. Here the outpoured leukocytes are soon replaced by histocytic cell formations. The fungi break into the retina through Bruch's membrane followed by these inflammatory cells. In contrast to this there is a conspicuous absence of blastospores in and around the retinal vessels. The retina's lack of primary involvement is of great advantage to the function of the eye as the uvea has all defense mechanisms available to combat the invaders. Our results show that there are apparently particular ways of blood supply to the retinal circulation.
    Notes: Zusammenfassung An Hand von histologischen Befunden, die bei Serienuntersuchungen von Kaninchenaugen nach intravenöser Injektion von Candida albicans-Suspensionen zur Beobachtung kamen, konnte gezeigt werden, daß die Einschleusung der Keime über die Ciliararterien erfolgt und die Infektion primär in der Aderhaut beginnt. Hier bilden sich nach einer vorübergehenden Ausschüttung von polymorphkernigen Leukocyten histiocytäre Granulome. Die Pilze wandern, gefolgt von den Entzündungszellen, durch die Bruchsche Membran in die Retina ein. In den Netzhautgefäßen finden sich hingegen praktisch nie Blastosporen. Für den Bestand des Auges ist dieser Weg, der die Netzhaut offensichtlich weitgehend verschont, von entscheidendem Vorteil, da in der Chorioidea alle Defensivkräfte mobilisiert werden können, die letzten Endes die Zerstörung der eingedrungenen Erreger bewirken.
    Type of Medium: Electronic Resource
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