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  • 2000-2004  (1)
  • 1985-1989  (1)
  • 1
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Listeria monocytogenes is a Gram-positive intracellular bacterium responsible for severe opportunistic infections in humans and animals. Signature-tagged mutagenesis (STM) was used to identify a gene named fbpA, required for efficient liver colonization of mice inoculated intravenously. FbpA was also shown to be required for intestinal and liver colonization after oral infection of transgenic mice expressing human E-cadherin. fbpA encodes a 570-amino-acid polypeptide that has strong homologies to atypical fibronectin-binding proteins. FbpA binds to immobilized human fibronectin in a dose-dependent and saturable manner and increases adherence of wild-type L. monocytogenes to HEp-2 cells in the presence of exogenous fibronectin. Despite the lack of conventional secretion/anchoring signals, FbpA is detected using an antibody generated against the recombinant FbpA protein on the bacterial surface by immunofluorescence, and in the membrane compartment by Western blot analysis of cell extracts. Strikingly, FbpA expression affects the protein levels of two virulence factors, listeriolysin O (LLO) and InlB, but not that of InlA or ActA. FbpA co-immunoprecipitates with LLO and InlB, but not with InlA or ActA. Thus, FbpA, in addition to being a fibronectin-binding protein, behaves as a chaperone or an escort protein for two important virulence factors and appears as a novel multifunctional virulence factor of L. monocytogenes.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Wir haben in Kulturüberständen einer Tn1545-induzierten, nicht-hämolysierenden Mutante vonListeria monocytogenes mittels Immunblotting mit Antiserum gegen gereinigtes Listeriolysin ein Eiweißmolekül von 52000D entdeckt (das sezernierte Listeriolysin O hat ein Molekulargewicht von 60000D). Die Insertionsstelle des Transposon wurde kloniert und einer Sequenzanalyse unterworfen. Das Transposon hatte an einem offenen Ableseraster (ORF) inseriert. Zwischen diesem ORF, Streptolysin O und Pneumolysin wurden Homologien festgestellt, die zeigen, daß das Transposon tatsächlich am Listeriolysin-Gen inseriert hatte. Da die nicht-hämolysierende Mutante avirulent war, konnten wir mit unserer Arbeit nachweisen, daß für die Virulenz die Anwesenheit des Listeriolysin-Gen oder seiner angrenzenden Region erforderlich ist.
    Notes: Summary In culture supernatants of a Tn 1545-induced non-hemolytic mutant ofListeria monocytogenes, by immunoblotting with an anti-serum raised against purified listeriolysin O, we have detected the presence of a truncated protein of 52,000D (the secreted listeriolysin O is 60,000D). The region of insertion of the transposon has been cloned and sequenced. The transposon had inserted in an open reading frame. The homologies detected between this ORF, streptolysin O and pneumolysin demonstrate the the transposon had indeed inserted in the listeriolysin O gene. As the non-hemolytic mutant was non-virulent, our work demonstrated that a genetic determinant essential for virulence is the listeriolysin O gene or its adjacent region.
    Type of Medium: Electronic Resource
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