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  • 2000-2004  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 80 (2002), S. 1835-1837 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We describe the theoretical and experimental investigation of mark shapes in thermally assisted or hybrid recording. The effect of alignment was studied by recording marks on perpendicular TbFeCo media using a commercial recording head and 840 nm laser. We found that mark shapes are primarily controlled through laser/head alignment, and that maximally straight transitions are obtained by placing the laser spot on the leading pole tip near the gap. Simulations of the recording process confirm the experimental findings and are used in conjunction with static write contours to show how transitions form. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Glutathione and its derivatives play an important role in the tolerance of plants against heavy metals. A glutathione transporter, BjGT1 (AJ561120), was cloned and functionally characterized from Brassica juncea, a plant which may be used for phytoremediation. The full-length BjGT1 cDNA showed homology with the high affinity glutathione transporter HGT1 from Saccharomyces cerevisiae and shares 92% identity with a putative glutathione transporter from A. thaliana (At4g16370). When expressed in the S. cerevisiae hgt1Δ strain, BjGT1 complemented the mutant on medium with glutathione as the only sulphur source and mediated the uptake of [3H]GSH. Immunoblot analysis with a peptide-specific antiserum directed against a C-terminal sequence revealed high BjGT1 expression in leaf tissue and relatively low expression in stem tissue, whereas BjGT1 protein was not detectable in root tissue. The amounts of BjGT1 mRNA and protein were analysed during a 6 d exposure of B. juncea to 25 µm Cd(NO3)2. BjGT1 mRNA was strongly induced by cadmium in stems and leaves. Unexpectedly, the amount of BjGT1 protein in leaves showed a pronounced decrease with a minimum after 96 h of Cd exposure, followed by partial recovery. The strong regulation of BjGT1 by cadmium suggests a role of this glutathione transporter during heavy metal exposure.
    Type of Medium: Electronic Resource
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