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  • 1
    ISSN: 1432-1246
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1279-8509
    Keywords: CLL MRD ; IgH genes rearrangement ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In the absence of specific chromosomal translocations the best method for detecting minimal residual disease (MRD) in B cell malignancies is based on the uniqueness of immunoglobulin (Ig) genes rearrangement. We here report a very sensitive method for assessing MRD in complete hematological remission (CHR) chronic lymphocytic leukemia (CLL) patients as defined by the international workshop on CLL (IWCLL). Patients: Twelve CLL patients in CHR and complete phenotypic remission (CPR) were included in the study. Eight of them received Fludarabine (FDR), one was treated by Chop regimen, and the remaining 3 were rescued by polychemotherapy followed by autologous bone marrow transplantation (ABMT). Methods: DNA extracted from peripheral blood lymphocytes (PBL) of each patient was amplified with VH family specific and framework 3 primers in 5′ and a consensus JH primer in 3′, before treatment and sequentially after the CPR completion. When no clonal rearrangement could be detected by this assay, the CDR3 sequence specific probe of the clone was used as the 3′ primer, associated to the VH family specific primer in 5′. PCR products were analyzed by classical procedures in agarose and/or acrylamide gels. Results: Mixtures of leukemic cells and normal PBL showed detection of a single leukemic cell among more than 105 normal cells. Four out of the 12 patients achieved molecular remission (MR) when employing CDR3 amplifification. All 3 autografted patients were in MR, whereas only one out of the 9 patients treated by chemotherapy alone achieved MR. When using a clone specific probe, a clonal signal was observed in all cases but one (ABMT). Results presented here confirm that MR may be achieved in a few cases of B-CLL. Further studies are needed to determine the exact relationship between MRD and clinical outcome.
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  • 3
    ISSN: 1600-5740
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The crystal structure of FeAlO3 has been determined at T = 298 K by neutron diffraction, using polycrystalline samples prepared in a high state of purity. The space group is Pna21, Z = 8; a = 4.9839 (1), b = 8.5544 (2), c = 9.2413 (2) Å. The structure, which is isomorphous to that of FeGaO3, can be described as a double combination of hexagonal and cubic closed packing of oxygen ions. There are four different cation sites labelled Fe1, Fe2 (predominantly occupied by iron), Al1 and Al2 (predominantly occupied by aluminium). The oxygen environment of Al1 forms an almost regular tetrahedron. The other sites have a distorted octahedral environment, especially irregular for Fe1 and Fe2. The fractions fi of iron ions over the four cation sites are: f1 = 0.78 (1), f2 = 0.76 (1), f3 = 0.10 (1) and f4 = 0.34 (1). Neutron diffraction at T = 30 K reveals a classical Néel ferrimagnetism, the direction of easy magnetization being a, with strong `180° cation-anion-cation' super-exchange antiferromagnetic interactions Fe1—O—Fe2 and Fe1—O—Al2 (Al2 being a site occupied by 0.34 Fe). The Néel sublattices are A = Fe1 + Al1 and B = Fe2 + Al2. The average magnetic moment per atom is weak (3.4 ± 0.3 μB) and the spontaneous magnetization at T = 30 K is extremely weak: 0.38 ± 0.17 μB per atom. Piezoelectricity probably originates in the bond arrangement of the four tetrahedral All sites in the unit cell, each tetrahedron being oriented with an Al1—O bond parallel to the polar c axis.
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  • 4
    ISSN: 1432-0878
    Keywords: Hyaluronan ; Hyaluronic acid-binding protein ; Microwaye fixation ; Histochemistry ; Urogenital tract ; Reproductive organs ; Rat (Sprague Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The histochemical distribution of hyaluronan was analysed in various urogenital organs of male and female (non-pregnant and pregnant) rats by use of a hyaluronan-binding protein and avidin biotin/peroxidase staining. Microwave-aided fixation was used to preserve the extracellular location of hyaluronan. The concentrations of hyaluronan in the different tissues were measured with a highly sensitive radio-assay. Hyaluronan accumulated predominantly in the connective tissue around smooth muscle fibres and in the subepithelial lamina propria. Abundant hyaluronan also occurred in perivascular and perineural connective tissue. In the female urogenital organs, hyaluronan content was high in the vagina and urinary bladder, and highest in the vagina during pregnancy. In the uterus, the surface epithelium of the endometrium stained intensely. In the ovary, the zona pellucida of the oocyte and the theca interna cell layer of the follicles and the follicular fluid of mature follicles exhibited prominent staining. The corpus luteum was devoid of hyaluronan, whereas enlarged corpora lutea of pregnancy exhibited weak, patchy staining. In male urogenital organs, staining for hyaluronan was absent from the testis and epididymis, whereas the erectile connective tissue of the penis stained intensely. The hyaluronan concentrations were high in penile tissue and urinary bladder, while testis, epididymis and the ductus deferens contained only little hyaluronan.
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  • 5
    ISSN: 1432-2072
    Keywords: Balanced placebo design ; Expectancy ; d-Amphetamine ; Subjective effects ; Self-administration ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The study examined the effects of expectation on the subjective effects and oral self-administration of 15 mgd-amphetamine (AMP) and placebo in 40 volunteers who reported no prior use of stimulants other than caffeine. A balanced placebo design was used to create four groups: told Placebo/got Placebo (P/P), told Placebo/got Stimulant (P/S), told Stimulant/got Placebo (S/P), told Stimulant/got Stimulant (S/S). There were three sessions. On one session (INFO), participants received a capsule containing AMP or placebo and were given information about the contents of the capsule according to the balanced placebo design. On another session (NO INFO), participants received no information about the capsule's contents and were given placebo. On the final session, participants were allowed to choose either the INFO or NO INFO capsule. Participants came to the laboratory to ingest their capsules, and then returned to their normal environments where they completed subjective effects questionnaires every 2 h for 8 h. Expectancies influenced the subjective effects reported during the INFO session, regardless of whether subjects actually received AMP or placebo: subjects who expected a stimulant had higher ratings of “feel drug” and “like drug”. The pharmacological effects of AMP were also evident on the INFO sessions: AMP produced its prototypic subjective effects regardless of subjects' expectancies. Significant interactions between drug and expectancy were obtained on self-report measures of anxiety and arousal: anxiety was higher for groups who received substances that did not match their expectations (P/S and S/P) and arousal increased most in volunteers who expected placebo but received stimulant. Choice of drug was determined primarily by pharmacology: participants who received AMP on the INFO session usually chose that capsule, regardless of information about its identity (P/S: 8/10; S/S: 9/10). In contrast, participants who received placebo on the INFO session chose this capsule at chance levels, regardless of information about its identity (S/P: 3/10; P/P: 6/10). Thus, expectancy influenced some of the subjective effects of AMP and placebo, but the pharmacological effects of the AMP were instrumental in determining whether volunteers would self-administer the drug.
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  • 6
    ISSN: 1432-0878
    Keywords: Key words: Hyaluronan ; Hyaluronic acid-binding protein ; Microwave fixation ; Histochemistry ; Urogenital tract ; Reproductive organs ; Rat (Sprague Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The histochemical distribution of hyaluronan was analysed in various urogenital organs of male and female (non-pregnant and pregnant) rats by use of a hyaluronan-binding protein and avidin biotin/peroxidase staining. Microwave-aided fixation was used to preserve the extracellular location of hyaluronan. The concentrations of hyaluronan in the different tissues were measured with a highly sensitive radio-assay. Hyaluronan accumulated predominantly in the connective tissue around smooth muscle fibres and in the subepithelial lamina propria. Abundant hyaluronan also occurred in perivascular and perineural connective tissue. In the female urogenital organs, hyaluronan content was high in the vagina and urinary bladder, and highest in the vagina during pregnancy. In the uterus, the surface epithelium of the endometrium stained intensely. In the ovary, the zona pellucida of the oocyte and the theca interna cell layer of the follicles and the follicular fluid of mature follicles exhibited prominent staining. The corpus luteum was devoid of hyaluronan, whereas enlarged corpora lutea of pregnancy exhibited weak, patchy staining. In male urogenital organs, staining for hyaluronan was absent from the testis and epididymis, whereas the erectile connective tissue of the penis stained intensely. The hyaluronan concentrations were high in penile tissue and urinary bladder, while testis, epididymis and the ductus deferens contained only little hyaluronan.
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  • 7
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The light emitted by an insulating material once its surface has been submitted to a cold plasma, plasma-induced luminescence is investigated on polypropylene films at low temperature. An analysis of the integral and wavelength resolved light is carried out. The kinetical evolution of the spectrum after plasma interaction are reported and analyzed. Investigation of the photo- and chemiluminescence spectra of the material shows that plasma-induced luminescence has three spectral components, each having different excitation mechanisms and thus different kinetics. The fastest is due to the ultraviolet irradiation during plasma contact, the second is dominated by radical chemistry producing carbonyl groups, and the third, with the slowest kinetics, is due to carrier recombination on the most conjugated chromophores which are preferentially charged by the plasma. To confirm the interpretation of plasma induced luminescence spectrum, the first results concerning short and long plasma interaction are considered. © 1997 American Institute of Physics.
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  • 8
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A new way is presented for deriving space-charge dynamics and transport parameters in thin films sandwiched between two metallic electrodes. The measurement method uses the detection of electroluminescence excited by square voltage pulses. The space-charge buildup time is derived from the electroluminescence time lag when the structure is excited by a single square voltage pulse. The relaxation time of the space charge is investigated by using a double pulse excitation. Carrier mobility is determined by an optical transit-time technique. Measurements are carried out on hydrogenated amorphous carbon layers with different composition and electrical properties. The films display space-charge limited conduction which is due to the existence of trap states. Their concentration is derived from the current–voltage characteristics and the mobility measurements. The trap concentration increases with a decreasing graphite content in the films. Film properties are weakly temperature dependent in graphite-rich layers as opposed to the behavior of layers with a lower graphite concentration. In this later case, the structural features of the films change at low temperature as suggested by the evolution of the trap concentration which decreases by three orders of magnitude, going from an exponential to a uniform distribution. © 1996 American Institute of Physics.
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