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  • 1
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Apolipoprotein(a) [apo(a)] contains a variable number of identical (K-IV A/B) or nearly identical (K-IV 1, K-IV 30–37) kringle repeats that are homologous to K-IV from plasminogen. The sizes of 414 apo(a) alleles were determined by pulsed-field gel electrophoresis (PFGE) of KpnI-digested DNA. Furthermore, sequence variation in the apo(a) K-IV 30–37 domain was analysed. Reverse transcription/polymerase chain reaction (RT-PCR) cloning of human liver poly A+ RNA followed by sequencing revealed a single nucleotide exchange in the ultimate K-IV (K-IV 37) of apo(a) (codon 4168); this results in an ATG (Met) to ACG (Thr) substitution. A PCR-based restriction assay of genomic DNA demonstrated that this substitution represents a common polymorphism. In 231 unrelated Tyroleans, the frequencies for the K-IV 37 Thr and K-IV 37 Met alleles were 0.66 and 0.34, respectively. The phase between the K-IV 37 Met/Thr and the KpnI size polymorphism was determined for 224 alleles. A significant linkage disequilibrium was detected between the sequence and size polymorphisms of apo(a). K-IV 37 Met was significantly associated with KpnI allele no. 18 (D AB= 0.0267 + 0.0101; χ2 = 10.09, df = 1). The Met/Thr polymorphism was further used to test whether deletions or duplications of K-IV 37 occur frequently in the apo(a) gene. Some 40 apo(a) alleles, 22 of which were from subjects that appeared to be double heterozygotes for K-IV repeat number and the Met/Thr variation were separated by PFGE and analysed for the 4168 Met/Thr polymorphism. The Met and Thr sequences were always present on different size alleles and no evidence for a duplication or deletion of K-IV 37 was obtained. This suggests that the copy number of K-IV 37 is invariable, in contrast to the highly variable K-IV A/B domain of the gene. The 4168 Met/Thr polymorphism had no effect on Lp(a) concentration, neither did it influence the lysine-binding property of the Lp(a) particle.
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  • 2
    ISSN: 1432-1076
    Keywords: Charcot-Marie-Tooth disease 1A ; Duplication on chromosome 17p11.2 ; Variable clinical phenotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Charcot-Marie-Tooth disease (CMT) was diagnosed by nerve conduction velocity and histology of the sural nerve in two boys aged 3 and 6 years with clinical signs of a severe neuromuscular disease. DNA analysis revealed the typical duplication on chromosome 17p 11.2 (2.7 kb allele) for CMT 1A. Although none of their family members reported symptoms of neuromuscular disease, the nerve conduction velocity was reduced in three members (father and two aunts). They were homozygous for the 2.7 kb allele and were assumed to carry three copies of this allele. The very differing clinical picture from one generation to the next in patients with identical neurophysiological and genetic results is discussed.
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  • 3
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 26 (1999), S. 0 
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The purpose of this investigation was to determine the relative erosion/solubility for hybrid glass–ionomers and a compomer using the jet-impingement technique with lactic acid of various molarities. The materials studied included Dyract®, Duet®, Vitremer®, Advance®, Fuji-II LC®, and Fuji-I®, Ketac Cem® and Modern Tenacin® as the control. Data were analysed using anova and Tukey's comparison test. Results indicated that depth of erosion/solubility with hybrid glass–ionomers and compomers was not measurable by the normal procedure. Similarly, weight changes were not detectable at 0·002, 0·02 and 0·05 m (except for Advance® at 0·05 m). All glass–ionomers materials and the compomer revealed weight changes at 0·1 m after 24 h, in the following increasing order: Dyract®〈Fuji-II LC®=Duet®〈Advance®〈Vitremer®. It was concluded that weight loss is a better parameter to characterize these new hybrids glass–ionomers and compomers for erosion/solubility by jet-impingement. In addition, a lactic acid of minimum 0·1 m as a test solution is required to generate 24 h-data that can be measured in most laboratories and thus used for statistical comparison.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Computational economics 8 (1995), S. 81-93 
    ISSN: 1572-9974
    Keywords: Multi-agent systems ; (distributed) planning ; communication ; resource scheduling ; logistics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Economics
    Notes: Abstract The transportation domain is presented as a multi-agent scenario and the use of techniques of Distributed Artificial Intelligence (DAI) for solving cooperatively the hard problems that occur in this domain are demonstrated. After a motivation and a description of the domain we will address questions of cooperation between the agents, techniques for task decomposition and task allocation, and multi-agent planning and scheduling. By introducing a cooperation protocol, examples will be presented that show the utility of the approach. Finally, some implementation aspects will be considered.
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  • 6
    ISSN: 1435-1536
    Keywords: Key words Foam films ; film thickness ; contact angle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  In the present study a detailed investigation of the thickness and contact angles of foam films prepared from sodium dodecyl sulfate as a function of LiCl concentration was carried out. Concentration of LiCl was varied in a wide range from 0.05 to 2.0 M. It was found that in the investigated system Newton black foam films are formed at LiCl concentrations higher than 1.4 M. This observation is proved by contact angle measurements and calculated values for the change of interaction free energy between common and Newton black films.
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