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  • 1995-1999  (4)
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  • 1
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract • Purpose: To clarify whether the prolonged presence of perfluorochemicals (PFC) in the vitreous cavity causes oxidative tissue damage and inflammatory response of the retina and, if so, what the process is. • Methods: After three different perfluorochemicals [perfluorodecalin (C10F18), perfluorooctane (C8F18) and Fluosol-DA (corresponding to a 20% emulsion of 70% PFD and 30% perfluorotripropylamine, C9F21N)] had been in the vitreous cavity of rabbits for 2 weeks, lipid peroxide concentration and myeloperoxidase activity in the retina were determined. • Results: Whereas only Fluosol-DA showed significant oxidative damage, the inflammatory activity was significantly increased in all groups. • Conclusion: The increased myeloperoxidase activity and the observed oxidative damage of the retina seem to be the effect of both perfluorochemical-loaded macrophages and inflammatory-induced lipid peroxidation.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract • Purpose: This investigation was carried out to ascertain whether oxygen free radicals can influence the growth behaviour and consecutive lipid peroxidation of retinal pigment epithelium (RPE) cells in vitro and whether scavengers can counteract these effects. • Methods: The experimental model was based on calf RPE cells. Hypoxanthine/xanthine oxidase (HX/XO) and superoxide dismutase/catalase (SOD/CAT) served as the radical generating system and scavengers, respectively. The components were tested alone and in combination. Lipid peroxides were determined in culture supernatants by a thiobarbituric acid assay. • Results: Concentrations of up to 100 μmol/1 of HX alone and 500/1000 μU of XO alone, as well as the application of the scavengers without the radical generating system (HX/XO), had no effect. Doserelated reduction of cell growth and increase of lipid peroxidation were found with HX/XO treatment (single dose of 500 and 1000 μU/ml 24 h after seeding). After application of 500 or 1000 μU/ml of XO, CAT, when given alone (1200 U/ ml), counteracted the effect of the radicals on cell growth and lipid peroxidation; SOD (300 U/ml) had no effect. A combination of SOD and CAT was no better than the effect of CAT alone. • Conclusion: The prevention of radical-induced reduction of cell growth and lipid peroxidation by scavengers supports trials of therapy using antioxidants and/or free radical scavengers for various ocular syndromes with RPE involvement.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract • Purpose: An intravitreal injection of Fluosol-DA leads to a higher alteration of the macrophage system of the retina than perfluorooctane and perfluorodecalin administered by the same route. The difference may be due to different kinds of oxidative damage caused by the three chemicals. To test the validity of this assumption, the degree of cell alteration, expressed as reduction of cytoplasmic motility, caused by these three perfluorochemicals was examined. • Methods: Using the hepatic macrophage system of the rat, cell alteration was examined by magnetometry after intravenous application of various perfluorochemicals [emulsified perfluorodecalin (C10F18), perfluorooctane (C8F17Br) and Fluosol-DA (corresponding to a 20% emulsion of 70% perfluorodecalin and 30% perfluorotripropylamine, C9F21N)]. • Results: After administration of high doses, all perfluorochemicals led to cytoskeleton alteration. This alteration, expressed as retardation of the relaxation period of ferromagnetic iron oxide particles, was most pronounced after administration of Fluosol-DA. • Conclusion: The compromising effect of perfluoro-chemicals is dose dependent and differs among the three compounds tested, with Fluosol-DA showing the greatest decrease in cytoplasmic motility.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  • Background: Investigation of the effects of different perfluorochemicals (PFC) on cultured dorsal root ganglion (DRG) cells. • Method: DRG cell cultures from 9- to 11-day-old chicken embryos were exposed to emulsified perfluorodecalin (PFD; C10F18; 0.5%, 1% and 10%) or perfluorooctylbromide (PFO; C8F17Br; 0.5%, 1% and 10%). The cells were evaluated under phase-contrast optics after 30 h and 120 h for 0.5 and 1% and after 5 h for 10%. To study the integrity of neuronal cells, immunohistochemical labelling for neurofilaments (NF) and tubulin (TUB) was performed. • Results: Concentrations of 0.5% and 1% of PFD or PFO did not change immunohistochemical labelling of DRG cells. Co-cultured macrophages showed a foam cell response, presumably representing ingested PFC. At both concentrations PFD induced a weaker foam cell response than PFO. A concentration of 10% led to the death of DRG cells and macrophages within 5 h. • Conclusion: PFC caused a dose-dependent damage of neuronal cells. Co-cultured macrophages developed a foam cell response similar to that observed in vivo after prolonged presence of PFC in the vitreous body. These observations indicate that PFD and PFO may not be suitable for long-term vitreous replacement in vitreoretinal surgery. However, the model is limited by several factors: (1) there are physiological differences between DRG cells and retinal ganglion cells; (2) in vivo retinal ganglion cells are protected by the overlying tissues; (3) the PFC used in tissue culture must be emulsified.
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