Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Annual Reviews  (2)
  • Wiley-Blackwell  (2)
  • International Union of Crystallography (IUCr)
  • Munksgaard International Publishers
  • 1990-1994  (4)
Collection
Publisher
Years
Year
  • 1
    ISSN: 0730-2312
    Keywords: rat protein kinase C ; recombinant baculovirus ; antisera ; phorbol ester ; isoenzymes ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Expression of rat protein kinase C-δ (PKC-δ ) and PKC-ξ in insect cells using recombinant baculovirus resulted in the production of proteins with a molecular size of approximately 76 kD and 78 kD, respectively, as determined by immunoblotting with subtype-specific antisera. Although the PKC-ξ cDNA encoded for 592 amino acids, a 76 kD protein was also generated by in vitro transcription/translation. Extracts of cells expressing PKC-δ were able to bind phorbol ester to levels comparable to extracts of cells expressing PKC-α. No phorbol ester binding was, however, detected in insect cell extracts expressing PKC-ξ. However, similar levels of protein kinase activity were detected in lysates of cells expressing PKC-δ or PKC-ξ when protamine sulfate was used as exogenous substrate. Compared to protamine sulfate, both, myelin basic protein (MBP) or histone, were poor substrates for PKC-δ and PKC-ξ. In contrast to PKC-ξ, the PKC-δ enzyme activity phosphorylated MBP or histone in a phosphatidylserine-(PS)/diacylglycerol(DG)-dependent manner, albeit not to the same extent as PKC-α. Lack of stimulation of the enzyme activity of PKC-ξ by PS/DG, was confirmed by endogenous phosphorylation of insect cell proteins by PKC-ξ, whereas several insect cell proteins were phosphorylated by PKC-δ in a PS/DG-dependent manner, including a protein of 78 kD.Our data demonstrate that the 76 kD PKC-ξ, in contrast to PKC-δ, is unable to bind phorbol esters and displays a protein kinase activity that is independent of PS of PS/DG. In addition, staurosporine was about 2-4 order of magnitudes less effective in inhibiting the protein kinase activities of PKC-δ and PKC-δ when compared to PKC-ξ.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 0044-2313
    Keywords: Polyphosphides ; Zintl-phases M3X11 (M = Na, K, Rb, Cs; X = P, As) ; P113-, As113- cage anion ; preparation ; vibrational spectra ; Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Chemistry and Structural Chemistry of Phosphides and Polyphosphides. 53. Preparation, Properties, and Vibrational Spectra of the Cage Anions P113- and As113-The Zintl-phases M3X11 (M = Na, K, Rb, Cs; X = P, As) are prepared from the elements or from M3X7 and X. The compounds undergo a first-order phase transition from the crystalline to the plastically crystalline state. Unit cell and space group of both modifications and the transition temperature Tc are determined. The vibrational spectra of the crystalline compounds and the Raman spectrum of the P113- anion in en-solution as well are measured. The assignment of the frequencies is given, based on the 32-D3 symmetry of the X113- cage anion. Normal coordinate analysis is carried out in terms of Cartesian coordinates to avoid the problem of redundancies in using internal coordinates. The force constants [mdyn Å-1] obtained for the characteristic bonds r, s, and t are: fppr = 1.34, fpps = 1.20, fppt = 1.08; fAsAsr = 1.1, fAsAss = 0.91. Normal vibrations and the potential energy distribution (PED) are discussed.
    Notes: Die Zintl-Phasen M3X11 (M = Na, K, Rb, Cs; X = P, As) werden aus den Elementen oder aus M3X7 und X synthetisiert. Die Verbindungen durchlaufen einen Phasenübergang 1. Ordnung vom kristallinen zum plastisch-kristallinen Zustand. Elementarzelle und Raumgruppe beider Modifikationen sowie Übergangstemperaturen Tc wurden bestimmt. Die Schwingungsspektren der kristallinen Verbindungen sowie das Raman-Spektrum des Anions P113- in en-Lösung wurden gemessen. Die Zuordnung der Schwingungsfrequenzen gelingt auf der Basis der Symmetrie 32-D3 der Käfiganionen X113-. Die Normalkoordinatenanalyse erfolgte in kartesischen Koordinaten, wodurch das in inneren Koordinaten auftretende Redundanzproblem vermieden wird. Auf diesem Wege erhaltene Kraftkonstanten [mdyn Å-1] der charakteristischen Bindungen r, s und t sind: fppr = 1, 34, fpps = 1, 20, fppt = 1, 08; fAsAsr = 1, 1, fAsAss = 0, 96, fAsAst = 0, 91. Die Normalschwingungen und die Verteilung der potentiellen Energie (PED) werden diskutiert.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 44 (1990), S. 451-477 
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    ISSN: 0084-6589
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...